99m-Technetium binding site in bone marrow mononuclear cells

Suhett, Grazielle; Souza, Sérgio Augusto Lopes de; Carvalho, Adriana Bastos; Rachid, Rachel de Pinho; Cunha-e-Silva, Narcisa L.; Carvalho, Antônio Carlos Campos de; Fonseca, Léa Mirian Barbosa da; Goldenberg, Regina Coeli dos Santos; Gutfilen, Bianca

doi:10.1186/s13287-015-0107-0

Cited by 5 publications

(6 citation statements)

References 34 publications

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“…Analysis of the biodistribution was carried out using 99m Tc, which efficiently labels the mononuclear fraction of bone marrow cells and allows cell tracking after systemic injection [ 26 ]. Two hours after administration, both 99m Tc-BMMC-healthy and 99m Tc-BMMC-sil migrated preferentially to the liver in the SIL and C groups.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of bone marrow-derived mononuclear cells from healthy or silicotic donors on recipient silicosis mice

et al. 2017

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BackgroundAdministration of bone marrow mononuclear cells (BMMCs) modulates lung inflammation and fibrosis in experimental silicosis. However, no studies have evaluated whether silicosis affects the efficacy of autologous BMMCs treatment. We hypothesized that BMMCs obtained from healthy or silicotic mice may improve lung function, but they might affect the inflammatory and fibrotic processes differently in experimental silicosis.MethodsC57BL/6 mice were randomly divided into control (C) and silicosis (SIL) groups. Mice in the SIL group were instilled with silica particles intratracheally; the C animals received saline using the same protocol. On day 15, the animals were treated with saline (Sal) or BMMCs (2 × 106 cells) from healthy (BMMC-healthy) and silicotic (BMMC-sil) donors. Lung mechanics were measured, and lungs were collected for histology and molecular biology analysis.ResultsBMMCs obtained from healthy and silicotic donors presented similar percentages of cell populations. 99mTc-BMMCs tracking revealed preferential migration of cells to the liver, and only a few GFP+ BMMCs were observed in lung tissue 24 h after treatment, regardless of donor type. Both the SIL-BMMC-healthy and SIL-BMMC-sil groups showed improvement in lung function, a reduction in the fractional area of granuloma, and a decrease in the number of mononuclear and apoptotic cells in lung parenchyma. In addition, the number of F4/80+ macrophages, the levels of interleukin-1 beta and transforming growth factor beta, and collagen fiber content in granuloma were reduced in SIL-BMMC-healthy mice, whereas mRNA expression of MMP-9 and procollagen I and III was reduced in the SIL-BMMC-sil group.ConclusionsAdministration of BMMCs from healthy and silicotic donors reduced lung inflammation and fibrosis, thus improving lung function. In addition, BMMC-healthy exhibited a greater improvement in lung morpho-functional changes in murine model of silicosis.

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Section: Discussionmentioning

confidence: 99%

Therapeutic effects of bone marrow-derived mononuclear cells from healthy or silicotic donors on recipient silicosis mice

et al. 2017

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“…We observed that cells administered intravenously migrated mainly to the kidneys in groups subjected to IRI, albeit in a small amount, suggesting that injured kidney can secrete chemoattraction factors for recruitment of BMMCs, as shown in other studies related to cytokines secreted by the injured microenvironment, such as SDF1 or TGFβ1 [76, 77]. Tracking of injected BMMCs labeled with technetium was only possible during the first hours after administration due to the short half-life of Tc [20]. Therefore, we transplanted GFP+ BMMCs and localized them beyond 24 h. Only a small amount of GFP+ BMMCs was observed in the kidney (Fig.…”

Section: Discussionmentioning

confidence: 79%

“…BMMCs were labeled with 99m-technetium( 99m Tc) according to protocols described previously [20]. Briefly, 500 mL of sterile SnCl 2 solution was added to the cell suspension and the mixture was incubated at room temperature for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Bone Marrow–Derived Mononuclear Cell Therapy Accelerates Renal Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and Apoptotic Related Molecules

Ornellas¹,

Ornellas²,

Martini³

et al. 2017

Cell Physiol Biochem

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Background/Aims: We investigated the regenerative capacity of intravenous administration of bone marrow–derived mononuclear cells (BMMCs) in a rat model of bilateral renal ischemia/reperfusion (IR) injury and the involvement of inflammatory anti-inflammatory and other biological markers in this process. Methods: Rats were subjected to 1h bilateral renal pedicle clamping. BMMCs were injected i.v 1h after reperfusion and tracked by ^99mTc and GFP+ BMMCs. Twenty-four hours after reperfusion, renal function and histological changes were evaluated. The mRNA (real time PCR) and protein (ELISA and immuno-staining) expression of biological markers were analyzed. Results: Renal function and structure improved after infusion of BMMCs in the IR group (IR-C). Labeled BMMCs were found in the kidneys after therapy. The expression of inflammatory and biological markers (TLR-2, TRL-4, RAGE, IL-17, HMGB-1, KIM-1) were reduced and the expression of anti-inflammatory and antioxidant markers (IL-10, Nrf2, and HO-1) were increased in IR-C animals compared with IR untreated animals (IR-S). The apoptotic index diminished and the proliferation index increased in IR-C compared with IR-S. Conclusion: The results contribute to our understanding of the role of different biological players in morphofunctional renal improvement and cytoprotection in a post-ischemic reperfusion kidney injury model subjected to cellular therapy.

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“…In our studies, we used 99m Tc-ASCs to evaluate cell homing. The choice of the 99m Tc was due to its characteristics such as short decay time, easy image capture through the use of gamma cameras ensuring optimum image quality, and high sensitivity and availability when compared to other types [62][63][64][65]. Our results showed that 99m Tc-ASCs+HA remained at the injection site for a period of twenty-four hours ( Figure 6), with no signs of dispersion or migration to different organs, showing its feasibility.…”

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confidence: 84%

Safety and Localization of Mesenchymal Stromal Cells Derived from Human Adipose Tissue-Associated Hyaluronic Acid: A Preclinical Study

Machado

Piñeiro²,

Ramos

et al. 2020

Stem Cells International

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Millions of plastic surgeries are performed worldwide every year with the objective of correcting lipodystrophies stemming from lesions, tumor resections, birth defects, and AIDS-associated antiretroviral therapy. Besides that, a large number of clinical research have assessed the outcome of procedures that rely on combinations of dermal fillers and autologous cells. However, little is known about the safety of these combinations and the localization of the injected cells. The aim of this study was to test the toxicity of a solution containing 1% hyaluronic acid (HA) and adipose-derived stromal cells (ASCs) from the human adipose tissue and to assess the localization of the injected cells, with and without HA, labeled with technetium-99m. Rats received subcutaneous and intraperitoneal injections of a solution containing 1% HA/adipose-derived stromal cells isolated from the human fat tissue. The animals were then observed for up to forty-two days. The solution tested in this study did not result in systemic, biochemical, or anatomic alterations that could represent toxicity symptoms. The association of HA and ASCs labeled with technetium-99m remained at the site of the injection within a period of twenty-four hours, as demonstrated by a whole-body imaging software fusion of SPECT and CT. In conclusion, our study shows that the subcutaneous and intraperitoneal injection of HA associated with adipose-derived stromal cells (ASCs) is safe. The association of HA and ASCs did not induce local or systemic toxicity. Thus, the administration of volume equal to or less than 0.2 mL of the agent filler (1×106 ASC+HA 1%) should be considered for subsequent studies and may be an alternative to dermal fillers due to the expected lasting effects.

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99m-Technetium binding site in bone marrow mononuclear cells

Cited by 5 publications

References 34 publications

Therapeutic effects of bone marrow-derived mononuclear cells from healthy or silicotic donors on recipient silicosis mice

Therapeutic effects of bone marrow-derived mononuclear cells from healthy or silicotic donors on recipient silicosis mice

Bone Marrow–Derived Mononuclear Cell Therapy Accelerates Renal Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and Apoptotic Related Molecules

Safety and Localization of Mesenchymal Stromal Cells Derived from Human Adipose Tissue-Associated Hyaluronic Acid: A Preclinical Study

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