[99mTc]Annexin V-128 SPECT Monitoring of Splenic and Disseminated Listeriosis in Mice: a Model of Imaging Sepsis

Hardy, Jonathan; Levashova, Zoia; Schmidt, Taly Gilat; Contag, Christopher H.; Blankenberg, Francis G.

doi:10.1007/s11307-014-0804-6

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…We observed nonspecific clinical signs such as apathy, anorexia, matted fur, conjunctivitis, and tremors. These findings were consistent with those of Hardy et al (2015), 4 d after an intravenous challenge of mice with Lis. monocytogenes (10 5 cfu).…”

Section: Short Communicationsupporting

confidence: 91%

Short communication: In vitro and in vivo probiotic potential of Lactobacillus plantarum B7 and Lactobacillus rhamnosus D1 isolated from Minas artisanal cheese

Valente

Acúrcio

Freitas

et al. 2019

Journal of Dairy Science

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Some Lactobacillus strains may contribute to the health of the host when administered in adequate concentrations, demonstrating their probiotic potential. In contrast, Listeria monocytogenes is a foodborne pathogen that can cause enteropathy, meningoencephalitis, abortion, and septicemia. The aim of this survey was to evaluate the in vitro and in vivo probiotic potential of Lactobacillus plantarum B7 and Lactobacillus rhamnosus D1, isolated from Minas artisanal cheese of the Serra da Canastra (Minas Gerais, Brazil), against Lis. monocytogenes. We submitted B7 and D1 to in vitro testing (antibiogram, tolerance to bile salts and artificial gastric fluid, and spot-on-lawn) and in vivo testing (relative weight gain in mice). Both Lactobacillus strains demonstrated in vitro inhibitory activity against Lis. monocytogenes, as well as sensitivity to antimicrobials and resistance to gastric acids and bile salts. In the in vivo assays, mice treated with D1 gained more weight than mice in the other groups. These results indicate that D1 could have higher probiotic potential than B7 because improvements in feed conversion may help animals fight infection.

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Section: Short Communicationsupporting

confidence: 91%

Short communication: In vitro and in vivo probiotic potential of Lactobacillus plantarum B7 and Lactobacillus rhamnosus D1 isolated from Minas artisanal cheese

Valente

Acúrcio

Freitas

et al. 2019

Journal of Dairy Science

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“…However, the number of pulmonary apoptotic cells did not differ from previously published murine ILD studies [ 39 , 43 , 55 ]. In comparison, studies in which 99m Tc-rhAnnexin V-128 SPECT/CT had been used to detect acute myocardial infarction, allograft rejection or infectious/septic states in vivo [ 32 , 33 , 36 , 47 ] showed substantially higher percentages or amounts of apoptotic cells per tissue area. In general, nuclear imaging of lung pathology compared with solid organs has inherent challenges, especially in small animals with very rapid breathing rates.…”

Section: Discussionmentioning

confidence: 99%

“…by the analysis of defined regions of interest [ 34 – 36 ]. Additionally, 99m Tc-rhAnnexin V-128 SPECT/CT has also shown some promise for the monitoring of therapeutic responses and overall disease outcome in, for example, infectious or cardiac diseases [ 32 , 47 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of 99mTc-rhAnnexin V-128 SPECT/CT as a diagnostic tool for early stages of interstitial lung disease associated with systemic sclerosis

et al. 2018

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BackgroundGiven the need for early detection of organ involvement in systemic sclerosis, we evaluated 99mTc-rhAnnexin V-128 for the detection of early stages of interstitial lung disease (ILD) in respective animal models using single photon emission computed tomography (SPECT/CT).MethodsIn bleomycin (BLM)-challenged mice, fos-related antigen 2 (Fra-2) transgenic (tg) mice and respective controls, lung injury was evaluated by analysis of hematoxylin and eosin (HE) and Sirius red staining, with semi-quantification of fibrosis by the Ashcroft score. Apoptotic cells were identified by TUNEL assay, cleaved caspase 3 staining and double staining with specific cell markers. To detect early stages of lung remodeling by visualization of apoptosis, mice were injected intravenously with 99mTc-rhAnnexin V-128 and imaged by small animal SPECT/CT. For confirmation, biodistribution and ex vivo autoradiography studies were performed.ResultsIn BLM-induced lung fibrosis, inflammatory infiltrates occurred as early as day 3 with peak at day 7, whereas pulmonary fibrosis developed from day 7 and was most pronounced at day 21. In accordance, the number of apoptotic cells was highest at day 3 compared with saline controls and then decreased over time. Epithelial cells (E-cadherin+) and inflammatory cells (CD45+) were the primary cells undergoing apoptosis in the earliest remodeling stages of experimental ILD. This was also true in the pathophysiologically different Fra-2 tg mice, where apoptosis of CD45+ cells occurred in the inflammatory stage. In accordance with the findings on tissue level, at day 3 in the BLM and at week 16 in the Fra-2 tg model, biodistribution and/or ex vivo autoradiography showed increased pulmonary uptake of 99mTc-rhAnnexin V-128 compared with controls. However, accumulation of the radiotracer and thus the signal intensity in lungs was too low to allow the differentiation of healthy and injured lungs in vivo.ConclusionAt the tissue level, 99mTc-rhAnnexin V-128 successfully demonstrated early stages of ILD in two animal models by detection of apoptotic epithelial and/or inflammatory cells. In vivo, however, we did not detect early lung injury. It remains to be investigated whether the same applies to human ILD.Electronic supplementary materialThe online version of this article (10.1186/s13075-018-1681-1) contains supplementary material, which is available to authorized users.

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“…It has an established role in the imaging of myocardial and cerebral perfusion but its role in infection is evolving. For example, the radioisotope, [99mTc]annexin V-128, is an in vivo marker of cellular stress and apoptosis, and can be used to detect and trace bacterial infection and response to treatment by SPECT imaging [10].…”

Section: Pre-clinical Imaging Modalities In Infectious Disease Researchmentioning

confidence: 99%

The power of bioluminescence imaging in understanding host-pathogen interactions

Suff

Waddington

2017

Methods

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Infectious diseases are one of the leading causes of death worldwide. Modelling and understanding human infection is imperative to developing treatments to reduce the global burden of infectious disease. Bioluminescence imaging is a highly sensitive, non-invasive technique based on the detection of light, produced by luciferase-catalysed reactions. In the study of infectious disease, bioluminescence imaging is a well-established technique; it can be used to detect, localize and quantify specific immune cells, pathogens or immunological processes. This enables longitudinal studies in which the spectrum of the disease process and its response to therapies can be monitored. Light producing transgenic rodents are emerging as key tools in the study of host response to infection. Here, we review the strategies for identifying biological processes in vivo, including the technology of bioluminescence imaging and illustrate how this technique is shedding light on the host-pathogen relationship.

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[99mTc]Annexin V-128 SPECT Monitoring of Splenic and Disseminated Listeriosis in Mice: a Model of Imaging Sepsis

Cited by 8 publications

References 26 publications

Short communication: In vitro and in vivo probiotic potential of Lactobacillus plantarum B7 and Lactobacillus rhamnosus D1 isolated from Minas artisanal cheese

Short communication: In vitro and in vivo probiotic potential of Lactobacillus plantarum B7 and Lactobacillus rhamnosus D1 isolated from Minas artisanal cheese

Evaluation of 99mTc-rhAnnexin V-128 SPECT/CT as a diagnostic tool for early stages of interstitial lung disease associated with systemic sclerosis

The power of bioluminescence imaging in understanding host-pathogen interactions

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