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Abstract

Aim:The main aim of the present work is to develop and validate a new simpler, selective, specific, and robust high performance liquid chromatographic method for the separation of Eliglustat and its isomers. Materials and Methods: For the accurate quantification method, for the all isomers was developed by performing the significant number of methods on trial and error techniques using a large number of polar and non-polar solvent mixtures as mobile phase. A distinctive resolution between Eliglustat and its stereo isomers was achieved on Immobilized Amylose tris (3-chloro-4-methylphenylcarbamate) stationary phase namely CHIRALPAK IF-3(4.6 mm × 250 mm, 3 µm), with flow rate of 1.0 mL/min using isocratic method containing n-