Untitled

Abstract

The mitochondrial DNA cytochrome oxidase subunit I (COI) gene sequences were used as a DNA barcode to identify species of the Capoeta genus in Anatolia and to clarify their systematics. A 652-bp region of the mitochondrial cytochrome oxidase subunit I (COI) was evaluated on 253 individuals representing seventeen species of Capoeta and thirty-three haplotypes were totally identified. The COI barcoding efficiency test for Anatolian Capoeta species provided twelve congruent numbers of putative species for four delimitation methods (BCM, K2P, ABGD, and NJ). The mean congeneric genetic distance (4.01%) was approximately 14-fold higher than the mean of conspecific one (0.28%) and there was a clear-cut barcode gap (0.92-1.26%) between maximum intraspecific and minimum nearest neighbour distance for Anatolian Capoeta species. The ML and BI phylogenies indicated a consensus tree topology containing three clades corresponding to the geographical origins: Anatolian-Iranian, Mesopotamian and Aralo-Caspian groups. The results of present study indicated that the COI gene could be a suitable DNA barcode marker for the most of scrapers species identification and delimitation with approximately 81% success rate.

DNA barcoding and species delimitation of the genus Oxynoemacheilus (Teleostei: Nemacheilidae) in Anatolia

“…DNA barcoding success is often related to the presence of a barcoding gap and DNA barcoding efficacy is lost when the barcoding gap is small or absent (Meyer & Paulay, 2005). However, the COI-based threshold value of 1.46% (Table 1) corresponds to the genetic distance range (0.9%-1.6%; Adeoba et al, 2018;Levin et al, 2018;Bektas et al, 2019) where there is significant overlap between intraspecific and congeneric values for some Cyprinid genera.…”

DNA barcoding revealed a high percentage of mislabeling in commercial fish products: the first empirical survey in South Texas

“…A DNA barcoding survey on fish mislabeling can choose one of the two strategies: testing a wide variety of species (Lakra et al, 2016; Pardo et al, 2018; Smith et al, 2008) or focusing on a specific group of fish (Bektas et al, 2019; Di Pinto et al, 2013; Lowenstein et al, 2009). We employed the former strategy since the present study was the first DNA barcoding survey specific to this geographical area to our knowledge.…”

A new Parakneria Poll 1965 (Gonorhynchiformes: Kneriidae), ‘Mikinkidi’ from the Upper Lufira Basin (Upper Congo: DRC): Evidence from a morphologic and DNA barcoding integrative approach

“…Furthermore, average Kimura 2-parameter (K2P) (Kimura, 1980) and uncorrected p (Srivathsan & Meier, 2012) genetic distances between and within the studied Parakneria species were calculated with the MEGA_X_10.0.5 program (Kumar et al, 2018). These two metric distances are routinely reported in DNA barcoding studies and thus enable direct comparison with other studies (see also Bektas et al, 2018;Levin et al, 2018). Both values are reported because the K2P distances tend to exaggerate species delimitation based on closely related sequences, whereas the p distance does not account for multiple mutation hits in strongly divergent sequences (Srivathsan & Meier, 2012).…”