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Abstract

The genus Ulva is one of the most numerous of marine and estuarine genera. Traditionally cultivated for human consumption, since the 1990s Ulva was integrated into land based Integrated Multi-Trophic Aquacultures (IMTA) for biomass production and bioremediation. A proper taxonomic identification is the first critical step in implementing an algal production program. However, Ulva species are difficult to morphological identify due to their phenotypic plasticity. Combined molecular and morphological techniques can lead to better characterization of Ulva sp. This study identifies the Ulva sp. cultivated in earth ponds facing the Ria Formosa Lagoon (South Portugal) as well as green algae growing spontaneously in the ponds. DNA barcoding with the markers ITS (Internal Transcribed Spacer) identified six species, with Ulva flexuosa being the cultivated one. Ulva flexuosa was recorded for the first time in South Portugal. However, taxonomic questions were raised because distinct clades were found for this species using published sequences. The 'lettuce-leaf' morphotype observed is not attributable to any of the marine subspecies of Ulva flexuosa.