9t18:1 and 11t18:1 activate the MAPK pathway to regulate the expression of PLA2 and cause inflammation in HUVECs

Hu, Sheng-Ben; Zou, Qian; Lv, Xiongwen; Zhou, Ruo-Lin; Xian, Niu; Weng, Chih‐Huang; Chen, Fang; Fan, Yanting; Deng, Zeyuan; Li, Jing

doi:10.1039/c9fo01982k

Cited by 15 publications

(17 citation statements)

References 46 publications

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“…Another recent study in HUVECs showed an increased protein expression of ICAM-1, VCAM-1, IL-6, and COX-2 and increased prostaglandin E 2 secretion in cells exposed to TVA or EA (100 µM for 24 h) compared to control, although the inflammatory responses induced by EA were significantly higher than the ones observed with the TVA treatment. This study also reported that these TFAs induced reduced cell viability and cell membrane damage [41], in agreement with our observations for these two FAs at 100 µM.…”

Section: Discussionsupporting

confidence: 93%

Differential Effects of Ruminant and Industrial 18-Carbon trans-Monounsaturated Fatty Acids (trans Vaccenic and Elaidic) on the Inflammatory Responses of an Endothelial Cell Line

et al. 2021

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Endothelial dysfunction and inflammation are recognised factors in the development of atherosclerosis. Evidence suggests that intake of industrial trans fatty acids (TFAs) promotes endothelial dysfunction, while ruminant TFAs may have the opposite effect. The aim of this study was to compare the effects of elaidic acid (EA (18:1n-9t); an industrially produced TFA) and trans vaccenic acid (TVA (18:1n-7t); a natural TFA found in ruminant milk and meat) on inflammatory responses of endothelial cells (ECs). ECs (EA.hy926 cells) were cultured under standard conditions and exposed to TFAs (1 to 50 μM) for 48 h. Then, the cells were cultured for a further 6 or 24 h with tumour necrosis factor alpha (TNF-α, 1 ng/mL) as an inflammatory stimulant. ECs remained viable after treatments. TFAs were incorporated into ECs in a dose-dependent manner. Preincubation with EA (50 µM) increased production of MCP-1, RANTES, and IL-8 in response to TNF-α, while preincubation with TVA (1 µM) decreased production of ICAM-1 and RANTES in response to TNF-α. Preincubation with EA (50 µM) upregulated toll-like receptor 4 and cyclooxygenase 2 gene expression in response to TNF-α. In contrast, preincubation with TVA (1 µM) downregulated TNF-α induced nuclear factor kappa B subunit 1 gene expression. Preincubation of ECs with EA (50 µM) increased THP-1 monocyte adhesion. In contrast, preincubation of ECs with TVA (1 µM) reduced THP-1 monocyte adhesion, while preincubation of ECs with TVA (50 µM) decreased the level of surface expression of ICAM-1 seen following TNF-α stimulation. The results suggest that TVA has some anti-inflammatory properties, while EA enhances the response to an inflammatory stimulus. These findings suggest differential effects induced by the TFAs tested, fitting with the idea that industrial TFAs and ruminant TFAs can have different and perhaps opposing biological actions in an inflammatory context.

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Section: Discussionsupporting

confidence: 93%

Differential Effects of Ruminant and Industrial 18-Carbon trans-Monounsaturated Fatty Acids (trans Vaccenic and Elaidic) on the Inflammatory Responses of an Endothelial Cell Line

et al. 2021

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“…Based on these results, we assume that PLA2 may involved in the ICAM-1, IL-8 and E-selectin expression in EA induced HAECs inflammation. This results were coordinate with Hu's report (32), and we first revealed that PLA2 may not participate in EA induced e-NOS expression in HAECs.…”

Section: Discussionsupporting

confidence: 90%

Protective Effect of Se-Methylselenocysteine on Elaidic Acid-Induced Inflammation in Human Arterial Endothelial Cells

Xia

Wen-yuan

et al. 2020

J Nutr Sci Vitaminol

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“…However, the damage by elaidic acid was significantly stronger than that by vaccenic acid. The expression of ICAM-1, VCAM-1, and IL-6 and the PGE secretion were increased and the effect of elaidic acid was significantly higher than that of vaccenic acid [103].…”

Section: Inflammationmentioning

confidence: 88%

“…TFAs have been reported to promote vascular diseases by promoting apoptosis and inflammation of vascular endothelial cells. Hu et al [103] investigated the effects of elaidic acid and vaccenic acid on human umbilical vein endothelial cell (HUVEC) function. The results showed that both elaidic acid and vaccenic acid reduced HUVEC viability, induced cell membrane damage, and increased membrane permeability.…”

Section: Inflammationmentioning

confidence: 99%

The Effect of Trans Fatty Acids on Human Health: Regulation and Consumption Patterns

Pipoyan

Stepanyan

et al. 2021

Foods

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Health effects of trans fatty acids (TFAs) on human organisms can vary according to their type, structure, composition, and origin. Even though the adverse health effects of industrial TFAs (iTFAs) have been widely discussed, the health effects of natural TFAs (nTFAs) are still questionable. Hence, it is important to review the literature and provide an overall picture on the health effects of different TFAs coming from industrial and ruminant sources, underlining those types that have adverse health effects as well as suggesting methods for reducing their harmful effects. Multiple databases (PubMed, Medline, Cochrane Library, etc.) were searched with the key words “trans fatty acid sources”, “ruminant”, “industrial”, “conjugated trans linoleic acid”, “human”, “coronary heart disease”, “cancer”, etc. Reference lists of the studies were scanned discussing the health effects of iTFAs and nTFAs. The review of the literature showed that iTFAs are found to be more harmful than ruminant-produced nTFAs. Although several beneficial effects (such as reduced risk of diabetes) for nTFAs have been observed, they should be used with caution. Since during labeling it is usually not mentioned whether the TFAs contained in food are of industrial or natural origin, the general suggestion is to reduce their consumption.

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9t18:1 and 11t18:1 activate the MAPK pathway to regulate the expression of PLA2 and cause inflammation in HUVECs

Abstract: trans fatty acids (TFAs) have been reported to promote vascular diseases mainly by promoting apoptosis and inflammation of vascular endothelial cells.

Cited by 15 publications

References 46 publications

Differential Effects of Ruminant and Industrial 18-Carbon trans-Monounsaturated Fatty Acids (trans Vaccenic and Elaidic) on the Inflammatory Responses of an Endothelial Cell Line

Differential Effects of Ruminant and Industrial 18-Carbon trans-Monounsaturated Fatty Acids (trans Vaccenic and Elaidic) on the Inflammatory Responses of an Endothelial Cell Line

Protective Effect of Se-Methylselenocysteine on Elaidic Acid-Induced Inflammation in Human Arterial Endothelial Cells

The Effect of Trans Fatty Acids on Human Health: Regulation and Consumption Patterns

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