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Llamoca-Zárate, Rómulo M.; Studart-Guimarães, Claúdia; Landsmann, J.; Campos, Francisco

doi:10.1023/a:1006315729266

Cited by 19 publications

(3 citation statements)

References 5 publications

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“…The genetic transformation of nopal is a very difficult process, as has been reported previously (Llamoca-Zarate et al 1998, 1999a, b, Silos-Espino et al 2006; Angulo-Bejarano 2013). In particular, genetic transformation by particle bombardment is marked by low efficiency of stably transformed cells, and thus, condition optimization is necessary.…”

Section: Discussionmentioning

confidence: 73%

“…The transient expression of uidA and nptII genes was described, but no stable integration of these genes was registered. In a second attempt, they reported the transient expression of the uidA gene in shoot apical meristems from nopal without evidence for stable foreign DNA integration (Llamoca-Zarate et al 1999a, b). Another study depicted particle bombardment in nopal shoot apical meristems that were transiently transformed to express the uidA and Atahas gene.…”

Section: Introductionmentioning

confidence: 99%

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Factors affecting genetic transformation by particle bombardment of the prickly pear cactus (O. ficus-indica)

2019

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In the present study, a novel transformation protocol for Opuntia ficus-indica was generated by means of particle bombardment. The best conditions obtained were: 900 psi rupture disk pressure, 8 cm microprojectile travel distance, and 4 h of exposition to 0.2 M mannitol. For all experiments, gold particles coated with 1.0 µg/µL of pBI426 plasmid DNA were used. With all these conditions, a 23% of transformation efficiency in terms of regeneration in selection media (100 mg/L kanamycin) was obtained. Interestingly, the presence of both transgenes: nptII and uidA, by means of PCR and RT-PCR assays was detected. The regeneration percentage achieved in terms of stable integration for both genes was 10%. In addition, we also detected adequate amounts of β-glucuronidase activity by means of the GUS fluorometric assay. The procedure described in the present investigation reveals the feasibility of using nopal for the introduction, expression, and possible production of heterologous proteins.

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Factors affecting genetic transformation by particle bombardment of the prickly pear cactus (O. ficus-indica)

2019

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“…Since then, there have been numerous reports on in vitro culture of various cacti [6], [11]. However, the techniques for in vitro culture of cacti are still not well developed, and in vitro morphogenetic behavior is not well understood [12], [13], [14]. Explant type and position on the plant impart a strong influence on the in vitro morphogenetic response [8], [15].…”

Section: Introductionmentioning

confidence: 99%

A highly efficient in vitro propagation protocol for elephant tusk cactus: Coryphantha elephantidens (Lem.) Lem.

Bhau

Wakhlu

2015

Journal of Genetic Engineering and Biotechnology

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BackgroundElephant tusk cactus Coryphantha elephantidens (Lem.) Lem. is an important attractive ornamental cactus. The plant produces offshoots from tubercles very rarely, and the seedlings exhibit slow growth and susceptibility to damping off. Slow growth and high demand in the cactus industry lead to finding an alternate fast propagation method.ResultsAn innovative in vitro technique based on axillary bud proliferation has been developed for an ornamental cactus C. elephantidens (Lem.) Lem. Four different explant types formed multiple shoots on Murashige and Skoog (MS) medium. Of the two cytokinins, 6-Benzylaminopurine (BAP) and Kinetin (KN), BAP proved to be more effective for multiple shoot induction and shoot growth from different explant types. Longitudinally cut stem explants, when cultured on MS medium supplemented with 6.6 μM BAP give maximum axillary shoot proliferation (12.4 shoots). Type of explant significantly influenced the micropropagation rate. Type of carbon source used in the medium imparted a profound effect on shoot growth and dry weight. The maximum dry weight gain of the shoot was observed with 9% sucrose.ConclusionDevelopment of an efficient micropropagation protocol which can be used to produce more than 10,000 rooted plantlets in 150 days from a single longitudinally divided shoot explant.

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Proteomic analysis of Mammillaria gracilis Pfeiff. in vitro-grown cultures exposed to iso-osmotic NaCl and mannitol

Rogić

Horvatić

Tkalec

et al. 2015

Plant Cell Tiss Organ Cult

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Cited by 19 publications

References 5 publications

Factors affecting genetic transformation by particle bombardment of the prickly pear cactus (O. ficus-indica)

Factors affecting genetic transformation by particle bombardment of the prickly pear cactus (O. ficus-indica)

A highly efficient in vitro propagation protocol for elephant tusk cactus: Coryphantha elephantidens (Lem.) Lem.

Proteomic analysis of Mammillaria gracilis Pfeiff. in vitro-grown cultures exposed to iso-osmotic NaCl and mannitol

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