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Abstract

Bulb scales of Lilium longiflorum 'Easter lily' were cultured in vitro on Murashige and Skoog (MS) medium containing various combinations and concentrations of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA). The MS medium supplemented with 15 µM BA and 0.5 µM NAA produced the largest numbers of multiple shoots (100%) after one month of culture. The MS medium containing 5 µM indole-3-butyric acid was effective for induction of roots. When individual isolated leaves from regenerated shoots were transferred to MS medium supplemented with 2,4-dichlorophenoxyacetate (2,4-D) and NAA, a yellow compact callus was obtained. The calluses differentiated into adventitious shoots and a high frequency of shoot organogenesis (70%) was found on MS medium containing 18 µM 2,4-D with 14 shoots.

Potential utilization of filaments and ovules as explant sources in in vitro propagation of Lilium sp.

“…Application of IM-2, IM-4 and IPM-7 for filament and ovule explants for the adventitious shoot formation, was also comparable to other research results (Tables 1-5) using leaf and bulb scales in an in vitro culture of Lilium. Bulb scales of Lilium longiflorum cv Easter lily planted on MS medium fortified by 18.09 µM 2,4-D stimulated 70% shoot organogenesis with 14 shoots/explant (Kanchanapoom et al, 2011), Lilium 'Prato' explants regenerated on MS medium fortified by 8.87 µM N-6 benzyladenine (BA) and 2.69 µM NAA produced 6 shoots/explant with 5.1 cm shoot height (El-Naggar et al, 2012), leaves cultured on the MS medium with 5.37 µM NAA and 2.27 µM TDZ regenerated 5.05 shoots and 36.5 leaves after 14 weeks incubation (Saetiew and Umamanit, 2015), leaf explants of Lilium orientalis 'Starfigther' on MS culture medium with 2.27 µM TDZ and 45.24 µM 2,4-D produced 8.3 shoots/explant, 5.4 leaves/shoot (Youssef et al, 2019), bulb scales planted on MS medium added by 5.37 µM NAA and 4.44 µM BAP stimulated 100% growing percentage with 4.3 shoots/explant and 1.4 microbulbs of Lilium longiflorum , basal scale segments of 'Oriental' hybrid Lilium 'Ravena' planted on MS medium 2.69 µM NAA and 8.88 µM BAP produced maximum culture establishment up to 76.2%, 5.5 bublets/explant, 2.2 cm shoot length and 3.4 leaves (Rafiq et al, 2021). These above results gave evident that each explant had optimal regeneration results in a specific culture medium.…”

Somatic embryo induction and plantlet regeneration of Canna × generalis from immature zygotic embryo

“…Therefore, effectively improving the rate of somatic embryo acquisition and induction e ciency was the main problem of Canna by SE induction. The production of SE is strongly in uenced by explant sources, genotypes, culture conditions, exogenous growth regulators, and the plant growth regulators (PGRs) added to the culture medium (Kanchanapoom et al 2011). It is expected to provide new ideas and technical references for the study of gene function and genetic improvement of Canna.…”