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Denis, Sylvain; Augur, Christopher; Marin, Bernard; Roussos, Sévastianos

doi:10.1023/a:1008866113065

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…Tea, as the second most popular (after water) natural nonalcoholic beverage, is processed from the leaves of tea plants . Numerous studies have suggested that tea can prevent cancer and other neurodegenerative or cardiovascular diseases. − However, because of its high caffeine content, high tea intake can affect those sensitive to caffeine by increasing their anxiety, blood pressure, insomnia, etc. , …”

Section: Introductionmentioning

confidence: 99%

Caffeine Content and Related Gene Expression: Novel Insight into Caffeine Metabolism in Camellia Plants Containing Low, Normal, and High Caffeine Concentrations

Zhu

Chen

et al. 2019

J. Agric. Food Chem.

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Caffeine is a crucial secondary metabolic product in tea plants. Although the presence of caffeine in tea plants has been identified, the molecular mechanisms regulating relevant caffeine metabolism remain unclear. For the elucidation of the caffeine biosynthesis and catabolism in Camellia plants, fresh, germinated leaves from four Camellia plants with low (2), normal (1), and high (1) caffeine concentrations, namely, low-caffeine tea 1 (LCT1, Camellia crassicolumna), low-caffeine tea 2 (LCT2, C. crassicolumna), Shuchazao (SCZ, C. sinensis), and Yunkang 43 (YK43, C. sinensis) were used in this research. Transcriptome and purine alkaloids analyses of these Camellia leaves were performed using RNA-Seq and liquid chromatography−mass spectrometry (LC−MS). Moreover, 15 N-caffeine tracing was performed to determine the metabolic fate of caffeine in leaves of these plants. Caffeine content was correlated with related gene expression levels, and a quantitative real-time (qRT) PCR analysis of specific genes showed a consistent tendency with the obtained transcriptomic analysis. On the basis of the results of stable isotope-labeled tracer experiments, we discovered a degradation pathway of caffeine to theobromine. These findings could assist researchers in understanding the caffeine-related mechanisms in Camellia plants containing low, normal, and high caffeine content and be applied to caffeine regulation and breeding improvement in future research.

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Section: Introductionmentioning

confidence: 99%

Caffeine Content and Related Gene Expression: Novel Insight into Caffeine Metabolism in Camellia Plants Containing Low, Normal, and High Caffeine Concentrations

Zhu

Chen

et al. 2019

J. Agric. Food Chem.

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“…When Penicillium and Aspergillus were cultured in vitro, the rate of caffeine degradation reached almost 100% [64]. Unlike the caffeine degradation pathway in bacteria, the primary degradation pathway in fungi involves 7-demethylation of caffeine to theophylline [65], followed by 1-demethylation of theophylline to 3-methylxanthine [66,67], and finally, degradation of 3-methylxanthine to xanthine (route III in Figure 2). The conversion of caffeine to theophylline is the rate-limiting step in caffeine metabolism by fungi.…”

Section: Caffeine Metabolism In Fungimentioning

confidence: 99%

Caffeine Synthesis and Its Mechanism and Application by Microbial Degradation, A Review

Zhao

Wei

et al. 2023

Foods

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Caffeine is a metabolite derived from purine nucleotides, typically accounting for 2–5% of the dry weight of tea and 1–2% of the dry weight of coffee. In the tea and coffee plants, the main synthesis pathway of caffeine is a four-step sequence consisting of three methylation reactions and one nucleosidase reaction using xanthine as a precursor. In bacteria, caffeine degradation occurs mainly through the pathways of N-demethylation and C-8 oxidation. However, a study fully and systematically summarizing the metabolism and application of caffeine in microorganisms has not been established elsewhere. In the present study, we provide a review of the biosynthesis, microbial degradation, gene expression, and application of caffeine microbial degradation. The present review aims to further elaborate the mechanism of caffeine metabolism by microorganisms and explore the development prospects in this field.

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“…Caffeine is, present with significant levels in coffee, tea, cocoa and Cola genera. The pharmacological effects of caffeine are well known: stimulation of central nervous system, toxicity when fed excessively, and mutagenicity on microorganisms (3).…”

Section: Introductionmentioning

confidence: 99%

Caffeine degradation by Rhizopus delemar in packed bed column bioreactor using coffee husk as substrate

Tagliari

Sanson

Zanette

et al. 2003

Braz. J. Microbiol.

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Various microorganisms including bacteria, yeast and fungi can degrade caffeine. There are few publications about caffeine degradation pathway in filamentous fungi, mainly by solid-state fermentation (SSF). Studies were carried out on degradation of caffeine and their metabolites by filamentous fungi in SSF using coffee husk as substrate. The purpose of this work was to investigate the caffeine degradation pathway by Rhizopus delemar in packed bed column fermenter and to compare this degradation metabolism with glass flasks fermentation. The methylxanthines were quantified by HPLC analysis. The experiments were realized with the optimized conditions in previous experiments: pH 6.5, 28ºC, inoculation rate 10 6 spores/g substrate, aeration rate 60 mL/min and initial moisture 73%. Under these conditions, after 72 hous of fermentation was achieved only 0.19% of caffeine and 0.014% of theophylline in the coffee husk. The strain proved to be able for caffeine and theophylline degradation by SSF in packed bed column bioreactor.

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Untitled

Cited by 6 publications

References 11 publications

Caffeine Content and Related Gene Expression: Novel Insight into Caffeine Metabolism in Camellia Plants Containing Low, Normal, and High Caffeine Concentrations

Caffeine Content and Related Gene Expression: Novel Insight into Caffeine Metabolism in Camellia Plants Containing Low, Normal, and High Caffeine Concentrations

Caffeine Synthesis and Its Mechanism and Application by Microbial Degradation, A Review

Caffeine degradation by Rhizopus delemar in packed bed column bioreactor using coffee husk as substrate

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