Untitled

Abstract

The expression of genes encoding conglutin gamma and a leginsulin-like protein has been examined in narrow-leafed lupin, Lupinus angustifolius L. Conglutin gamma is a homologue of basic 7S globulin (Bg), the insulin and leginsulin binding protein from soybean. Accumulation of conglutin gamma mRNA, as assessed by northern assays and reverse-transcription PCR, was tightly regulated both spatially and temporally in lupin plants and was detected almost exclusively in developing seeds. Similar tissue and temporal specificity was demonstrated when 1.8 kb of the promoter region from the conglutin gamma gene was used to drive the expression of a beta-glucuronidase reporter gene in transgenic plants. In stably transformed tobacco the conglutin gamma promoter produced strong, temporally regulated and seed-specific expression of the reporter gene which was localised to the embryo tissues and to a layer of cells adjacent to the seed coat. A truncated 0.29 kb promoter fragment produced much reduced levels of expression and a loss of embryo specificity. Leginsulin-like mRNA was similarly detected in lupins only in developing seeds. The leginsulin-like gene detected in L. angustifolius showed 96% sequence identity to leginsulin from soybean within the 280 bp region amplified from lupin by PCR. The results demonstrate that both components of a Bg-leginsulin putative signal transduction pathway are present in the seeds of lupin.

Genome-wide analysis identifies gain and loss/change of function within the small multigenic insecticidal Albumin 1 family of Medicago truncatula

“…Sophora japonica , the Japanese pagoda tree) harbors the more divergent albumin I known to date. Consensus legume phylogeny therefore dates the A1b family back to the ancestor of S. japonicum and all other known families harbouring such peptides, especially the genistoid clade –genus Lupinus ; Uniprot Q96474 [ 46 ], with an estimated 56–58.5 My/late paleocene origin [ 47 ].…”

Draft Genome Sequence, and a Sequence-Defined Genetic Linkage Map of the Legume Crop Species Lupinus angustifolius L

“…However, the draft assembly is still valuable in studying the functional genes in lupin. For example, previous studies on lupin storage proteins were based upon gene expression (including mRNA and cDNA translations) [50], gene product (protein) isolation [51], or homology with storage proteins from other species [52]. In this study, we identified the scaffold sequences for each of the lupin storage proteins, which, for the first time, provided the genomic DNA sequences flanking the protein genes.…”

Pea Albumin 1 Subunit b (PA1b), a Promising Bioinsecticide of Plant Origin

“…There is little, or no, transcription of these genes in early development but then transcription progressively increases to reach its highest level midway through development (20 to 22 days after flowering), followed by a sharp decline [4,38]. In the genome of another legume, the narrow-leafed lupin ( Lupinus angustifolius ), a PA1b-like gene was detected that showed, within the same region, 96% sequence identity with the leginsulin gene from soybeans [39]. The gene was functional at the transcriptional level since the corresponding mRNAs were detected but they were in very low quantities and, almost exclusively, in developing seeds (mainly in cotyledons 40 days after anthesis) [39].…”

“…In the genome of another legume, the narrow-leafed lupin ( Lupinus angustifolius ), a PA1b-like gene was detected that showed, within the same region, 96% sequence identity with the leginsulin gene from soybeans [39]. The gene was functional at the transcriptional level since the corresponding mRNAs were detected but they were in very low quantities and, almost exclusively, in developing seeds (mainly in cotyledons 40 days after anthesis) [39]. However, unlike in soybeans [29], no evidence was found for the expression of the leginsulin-like gene in the radicle of lupins (two days after germination), suggesting a tight temporal regulation of the gene in developing and germinating seeds [39].…”