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Neary, Emine Turgut; Develi, Nermin; Özgül, Gülistan

doi:10.4194/1303-2712-v12_1_03

Cited by 3 publications

References 9 publications

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Development of a LAMP-LFD DNA biosensor for detecting the zoonotic monogenean Dactylogyrus spp. in goldfish (Carassius auratus)

Raksaman,

Tejangkura,

Purivirojkul

et al. 2024

Preprint

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The genus Dactylogyrus, comprising more than 900 taxa, is an important group of ectoparasites with high host specificity that causes disease and economic loss in ornamental fish. Dactylogyrus attaches to the host’s gills, causing respiratory disorders and potentially leading to fish mortality. This study was performed to develop a detection method for Dactylogyrus spp. infection in goldfish (Carassius auratus) using a loop-mediated isothermal amplification (LAMP) assay coupled with a lateral flow dipstick (LFD). The LAMP-LFD assay was performed in 75 minutes under isothermal conditions at 65°C. It demonstrated high specificity with no cross-amplification with related parasites or their definitive hosts. The sensitivity of the LAMP-LFD assay was 3 pg /µL for three Dactylogyrus species. Clinical sample examination showed that the accuracy of the LAMP-LFD assay was consistent with that of microscopy. In summary, the established LAMP-LFD assay targeting the 28S rDNA gene is a rapid and convenient method for detecting Dactylogyrus spp. in goldfish, aiding in parasite prevention, enhancing outbreak surveillance, and addressing veterinary health issues in the ornamental fish business.

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Development of a LAMP-LFD DNA biosensor for detecting the zoonotic monogenean Dactylogyrus spp. in goldfish (Carassius auratus)

Raksaman,

Tejangkura,

Purivirojkul

et al. 2024

Preprint

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In vitro Antiparasitic Activity of Ginger (Zingiber officinale) Bulb and Pomegranate (Punica granatum) Peel Against Monogenean Fish Parasite., Dactylogyrus sp.

Van

Yilmaz

Yavuzcan

2021

Acta Aquatica Turcica

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The Monogenean parasite, Dactylogyrus sp., is being considered as one of the most dangerous pathogens in freshwater fish with a high infestation in common carp (Cyprinus carpio). The treatment of parasites including Monogeneans is an important part of fish health maintenance in the overall cycle of aquaculture. Novel applications of natural plant products to eradicate the parasites have taken high attention in aquaculture since they are reported to have less adverse impacts on the environment and fish in comparison to other chemical treatments. In the present study, in vitro antiparasitic effects of the ginger (Zingiber officinale) bulb and pomegranate (Punica granatum) peel against Dactylogyrus sp. were investigated by using in vitro tests. Individuals of the parasite were exposed to different concentrations of ginger (10, 50, 100, and 250 mg/ml) and pomegranate peel (50, 100, 250, and 500 mg/ml) for a specified period. In vitro cumulative mortality values reached 100% in 5 minutes after exposure to ginger at the concentration of 250 mg/ml in 9 minutes after exposure to 100 mg/ml and 50 mg/ml. Cumulative mortality was 58% in 9 minutes after exposure to ginger at the concentration of 10 mg/ml. In in vitro pomegranate tests, cumulative mortality values were assessed 100% in 3 minutes after exposure to pomegranate peel at the concentration of 500 mg/ml and in 6 minutes after exposure to 250 mg/ml and 100 mg/ml. Cumulative mortality was 66% in 6 minutes after exposure to pomegranate peel at the concentration of 50 mg/ml. In vitro results revealed that ginger and pomegranate peel solutions had antiparasitic effects on Dactylogyrus sp. to some degree, depending on solution concentration and exposure time. The efficacy of the ginger and pomegranate peel solutions against Monogeneans should be confirmed in fish through in vivo tests.

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Squalius recurvirostris Özuluğ & Freyhof, 2011'in Dactylogyrid Parazit Faunası Üzerine Bir Araştırma

Elbay

Öztürk

2021

Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Bu çalışmanın amacı, Düzağaç-Akdeğirmen Baraj Gölü (Afyonkarahisar)'ndeki Squalius recurvirostris'in dactylogyrid parazit türlerini ve bu türlerin anatomik, morfolojik, metrik ve ekolojik özelliklerini tanımlamaktır. Araştırma metodolojisi kapsamında, Squalius recurvirostris örneklerinin deri yüzeyi, yüzgeç ve solungaçları dactylogyrid parazitler bakımından incelendi. Dactylogyrus sp. örneklerinin bazıları etil alkol ortamında muhafaza edilirken bazıları anatomik, morfolojik ve metrik özelliklerinin tanımlanması için daimi preparat yapıldı. Dactylogyrus naviculoides'in enfestasyon yüzdesi %89,4 ve ortalama parazit yoğunluğu 27,1±24,1 dir. Dactylogyrus vistulae için bu değerler sırasıyla %63,6 ve 4,5±2,3 dir. Her iki parazit türü tüm mevsimlerde kaydedildi. Dactylogyrus naviculoides'in yoğunluğu kış döneminde, Dactylogyrus vistulae'nin yoğunluğu ilkbaharda daha baskındı. Balık numuneleri yaş büyüklüğüne göre 5 gruba ayrıldı. Büyük yaş grubundaki balıklarda her iki Dactylogyrus türünün daha yüksek enfestasyona neden olduğu tespit edildi. Diğer yandan, her iki parazit türüne ait enfestasyon değerleri erkek balık bireylerinde dişilere göre daha yüksek değerde kaydedildi: Dactylogyrus naviculoides (%89,4, 27,1±24,1), Dactylogyrus vistulae (%63,6, 4,5±2,3). Sonuç olarak, Düzağaç-Akdeğirmen Baraj Gölü'ndeki Squalius recurvirostris'te 2 dactylogyrid parazit türü belirlenmiştir. Bu parazit türler, Squalius recurvirostris için yeni kayıttır. Böylece ilgili parazit türlerin coğrafik yayılışlarına yeni bir lokalite ve yeni bir konak ilave edilmiştir. Ayrıca, parazit türlerin enfestasyon olguları ekolojik yönden de değerlendirilmiştir.

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Untitled

Cited by 3 publications

References 9 publications

Development of a LAMP-LFD DNA biosensor for detecting the zoonotic monogenean Dactylogyrus spp. in goldfish (Carassius auratus)

Development of a LAMP-LFD DNA biosensor for detecting the zoonotic monogenean Dactylogyrus spp. in goldfish (Carassius auratus)

In vitro Antiparasitic Activity of Ginger (Zingiber officinale) Bulb and Pomegranate (Punica granatum) Peel Against Monogenean Fish Parasite., Dactylogyrus sp.

Squalius recurvirostris Özuluğ & Freyhof, 2011'in Dactylogyrid Parazit Faunası Üzerine Bir Araştırma

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