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Rasia‐Filho, Alberto A.; Fin, Cyntia; Alves, Marcelle Duarte; Possa, Marianne A.; Roehrig, Cíntia; Feóli, Ana Maria Pandolfo; Silva, Luciane; Perry, Marcos L. S.

doi:10.1023/a:1021666406738

Cited by 2 publications

References 35 publications

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Evidence for a synergistic action of glutaric and 3‐hydroxyglutaric acids disturbing rat brain energy metabolism

Ferreira

Tonin

Schuck

et al. 2007

Intl J of Devlp Neuroscience

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Glutaric acidemia type I is an inherited metabolic disorder caused by a severe deficiency of the mitochondrial glutaryl-CoA dehydrogenase activity leading to accumulation of predominantly glutaric and 3-hydroxyglutaric acids in the brain tissue of the affected patients. Considering that a toxic role was recently postulated for quinolinic acid in the neuropathology of glutaric acidemia type I, in the present work we investigated whether the combination of quinolinic acid with glutaric or 3-hydroxyglutaric acids or the mixture of glutaric plus 3-hydroxyglutaric acids could alter brain energy metabolism. The parameters evaluated in cerebral cortex from young rats were glucose utilization, lactate formation and (14)CO(2) production from labeled glucose and acetate, as well as the activities of pyruvate dehydrogenase and creatine kinase. We first observed that glutaric (5 mM), 3-hydroxyglutaric (1 mM) and quinolinic acids (0.1 microM) per se did not alter these parameters. Similarly, no change of these parameters occurred when combining glutaric with quinolinic acids or 3-hydroxyglutaric with quinolinic acids. In contrast, co-incubation of glutaric plus 3-hydroxyglutaric acids increased glucose utilization, decreased (14)CO(2) generation from glucose, inhibited pyruvate dehydrogenase activity as well as total and mitochondrial creatine kinase activities. The glutaric plus 3-hydroxyglutaric acids-induced inhibitory effects on creatine kinase were prevented by the antioxidants glutathione and catalase plus superoxide dismutase, indicating the participation of reactive oxygen species. Our data indicate a synergic action of glutaric and 3-hydroxyglutaric acids disturbing energy metabolism in cerebral cortex of young rats.

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Evidence for a synergistic action of glutaric and 3‐hydroxyglutaric acids disturbing rat brain energy metabolism

Ferreira

Tonin

Schuck

et al. 2007

Intl J of Devlp Neuroscience

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Sex-Related Gene Expression in the Posterodorsal Medial Amygdala of Cycling Female Rats Along with Prolactin Modulation of Lordosis Behavior

Bühler,

de Moura,

Giovenardi

et al. 2024

Preprint

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The rat posterodorsal medial amygdala (MePD) is sexually dimorphic, has a high concentration of receptors for gonadal hormones and prolactin (PRL), and modulates reproduction. To unravel genetic and functional data for this relevant node of the social behavior network, we studied the expression of ERalpha, ERbeta, GPER1, Kiss1, Kiss1R, PRGR, PRL, PRLR, EGR1, JAK2, STAT5A, and STAT5B in the MePD of males and females along the estrous cycle using the RT-qPCR technique. We also investigated whether PRL in the MePD would affect the sexual behavior display of proestrus females by microinjecting saline, the PRL receptor antagonist Del1-9-G129R-hPRL (1 microM and 10 microM), or PRL (1 nM) and Del1-9-G129R-hPRL (10 microM) 3h before the onset of the dark-cycle period. The estrogen-dependent lordosis behavior, indicative of sexual receptivity of proestrus females, was recorded and compared before (control) and after (test) microinjections in these groups. Sex differences were found in the right and left MePD gene expression. ERalpha and Kiss1R, as well as PRL, Short PRLR, and STAT5B expression is higher in cycling females than males. Kiss1 expression is higher in males than females, and GPER1 is higher during diestrus than proestrus. Furthermore, Del1-9-G129R-hPRL in the MePD significantly reduced the full display and quotient of lordosis in proestrus females, an effect restored by the co-microinjection of PRL. In conjunction, the expression of studied genes showed specific sex and estrous cycle phase features while, in proestrus, PRL action in the MePD plays an essential role in the display of lordosis during the ovulatory period.

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Cited by 2 publications

References 35 publications

Evidence for a synergistic action of glutaric and 3‐hydroxyglutaric acids disturbing rat brain energy metabolism

Evidence for a synergistic action of glutaric and 3‐hydroxyglutaric acids disturbing rat brain energy metabolism

Sex-Related Gene Expression in the Posterodorsal Medial Amygdala of Cycling Female Rats Along with Prolactin Modulation of Lordosis Behavior

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