Untitled

Abstract

The present study was developed a suitable protocol for callus induction and plant regeneration of Cleome gynandra L. from leaf explants. Callus was initiated from leaf explants on Murashige and Skoog (MS) medium supplemented with different concentration and combinations of plant growth regulators. They are using (5-25 µM) of BAP (6-benzyladenine), KN (kinetin), and TDZ (thidiazuron) cytokinins along with (2-10 µM) different auxins [NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid)]. Maximum regenerative callus (95%) was observed on TDZ + NAA (10 µM + 2 µM). Organogenic calli were transferred to shoot induction medium containing a different concentration of cytokinins (BAP, KIN, TDZ) ranging from 5-25 µM. Among the cytokinins tested BAP 10 µM produced number of shoots (35.2) with maximum shoot induction frequency (98%). Isolated shoots were carefully transferred to half-strength MS medium supplemented with auxins (IAA, IBA, and NAA) ranging from 2-10 µM. The maximum numbers of roots (8.8) were observed on 4 µM IBA. The rooted plantlets were successfully acclimatization.