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Abstract

Neurons of rat neocortex and spinal cord express NADPH-diaphorase after heterotopic allotransplantation into the sciatic nerve. These peculiarity of diaphorase expression in transplanted cells in comparison with brain cells developing in situ suggest an important role of afferent and efferent relationships in the development of NO mechanisms in neurons.

Astrogliogenesis in Heterotopic Allotransplants of Rat Embryonic Neocortex

“…The former gradually differentiated into GFAP-positive astrocytes, the latter after 30 days formed a multilayer glial lining consisting of vimentin-positive cells at the boundary with tissues of the recipient nerve. The formation of this lining in neurotransplants of the neocortex transplanted into the peripheral nerve has been previously reported by us [6,7,10]. A special study comparing the development of transplants from rat embryonic neocortex in the brain, anterior chamber of the eye, and peripheral nerve showed that this lining consisting of ependyma-like cells is typical only of transplants developing in the nerve [7].…”

“…The formation of this lining in neurotransplants of the neocortex transplanted into the peripheral nerve has been previously reported by us [6,7,10]. A special study comparing the development of transplants from rat embryonic neocortex in the brain, anterior chamber of the eye, and peripheral nerve showed that this lining consisting of ependyma-like cells is typical only of transplants developing in the nerve [7]. Here we showed immunohistochemically that most cells of the glial lining are presented by ependymocytes: it is known that the ependyma of cerebral ventricles in intact rats contain vimentin [4].…”

“…For instance, we observed degenerative changes in some nerve cells and their death that in delayed terms after transplantation [5,8]. Expression of glial fi brillary acidic protein (GFAP) in cells of neurotransplants was detected [7].…”

Study of mitotic activity and degeneration of cells in the dorsolateral wall of the anterior cerebral vesicle in rat embryos on the model of ectopic neurotransplantation

“…These data indicate that the microenvironment modulates proliferative activity of transplanted cells. Our previous studies showed that these features are related to the presence of the glial layer at the boundary between the transplant and nerve tissue of the recipient [4]. Environmental factors in the regenerating nerve probably modulate differentiation of several multipotent precursor cells into the glia.…”

Serotonin is involved in the regulation of histogenetic processes in rat embryonic neocortex

“…The majority of these cells did not differ from NEC by their morphology. By their maturity they did not reach the level of the neural elements in intact embryonic neocortical transplant (E14) of the corresponding developmental period [6,8]. They could be detected only by immunohistochemical reaction to NeuN (Fig.…”