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Azlan, G. Jualang; Marziah, M.; Radzali, M.; Johari, Rozita

doi:10.1023/a:1015662118877

Cited by 23 publications

(2 citation statements)

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“…A similar positive effect of a diluted nutrient medium on growth of transformed roots was also previously observed (Nussbaumer et al 1998;Sung and Huang 2000). In contrast, there are also reports, where no beneficial effects on root growth or the production of secondary metabolites were observed when diluting the nutrient content of the medium (Azlan et al 2002).…”

Section: Discussionsupporting

confidence: 79%

Alkamide production from hairy root cultures of Echinacea

Romero

Delate

Kraus

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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Hairy root cultures of Echinacea, one of the most important medicinal plants in the US, represent a valuable alternative to field cultivation for the production of bioactive secondary metabolites. In this study, the three most economically important species of Echinacea (Echinacea purpurea, Echinacea pallida, and Echinacea angustifolia) were readily transformed with two strains of Agrobacterium that produce the hairy root phenotype. Transformed roots of all three species exhibited consistent accelerated growth and increased levels of alkamide production. Optimization of the culture of Echinacea hairy roots was implemented to enhance both growth and alkamide production concomitantly. The use of halfstrength Gamborg's B5 medium supplemented with 3.0% sucrose was twice as effective in maintaining hairy root production than any other media tested. The addition of indolebutyric acid increased the growth rate of roots by as much as 14-fold. Alkamide production increased severalfold in response to the addition of the elicitor, jasmonic acid, but did not respond to the addition of indolebutyric acid. Induced accumulation of the important bioactive compounds, alkamides 2 and 8, was observed both in transformed roots and in response to jasmonic acid treatments. The results of this study demonstrate the efficacy of hairy root cultures of Echinacea for the in vitro production of alkamides and establish guidelines for optimum yield.

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Section: Discussionsupporting

confidence: 79%

Alkamide production from hairy root cultures of Echinacea

Romero

Delate

Kraus

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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“…The plant family of Solanaceae produces many secondary metabolites which are of immense pharmacological importance ( Hamill et al., 1986 ; Rhodes et al., 1986 ; Furze et al., 1987 ; Robins et al., 1987 ; Walton et al., 1988 ; Benson and Hamill, 1991 ; Jovanović et al., 1991 ; Fecker et al., 1992 ; Ikenaga et al., 1995 ; Zehra et al., 1998 ; Jualang Azlan et al., 2002 ; Okršlar et al., 2002 ; Komaraiah et al., 2003 ). Among these, alkaloids, and more specifically, tropane alkaloids are the ‘signature alkaloids’ and are found as major compounds in different genera of Solanaceae ( Zhang et al., 2023a ) Three main representatives of this group, hyoscyamine, atropine, and scopolamine whose production through hairy root culture have been investigated in detail.…”

Section: Pharmacologically Important Secondary Metabolites Production...mentioning

confidence: 99%

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Biswas,

Chakraborty,

Mukherjee

et al. 2023

Front. Plant Sci.

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Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants. ‘Hairy roots’, mirroring the same phytochemical pattern of the corresponding root of the parent plant with higher growth rate and productivity, are therefore extensively studied as an effective alternative for the in vitro production of these metabolites. Hairy roots are the transformed roots, generated from the infection site of the wounded plants with Agrobacterium rhizogenes. With their fast growth, being free from pathogen and herbicide contamination, genetic stability, and autotrophic nature for plant hormones, hairy roots are considered as useful bioproduction systems for specialized metabolites. Lately, several elicitation methods have been employed to enhance the accumulation of these compounds in the hairy root cultures for both small and large-scale production. Nevertheless, in the latter case, the cultivation of hairy roots in bioreactors should still be optimized. Hairy roots can also be utilized for metabolic engineering of the regulatory genes in the metabolic pathways leading to enhanced production of metabolites. The present study summarizes the updated and modern biotechnological aspects for enhanced production of secondary metabolites in the hairy root cultures of the plants of Solanaceae and their respective importance.

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Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

2013

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The history of Agrobacterium-related plant biotechnology goes back for more than three decades with the discovery of molecular mechanisms of crown gall disease in plants. After 1980s, gene technologies began developing rapidly and today, related with the improved gene transfer methods, plant biotechnology has become one of the most important branches in science. Till now, the most important genes related with agricultural affairs have been utilized for cloning of plants with the deployment of different techniques used in genetic engineering. Especially, Agrobacterium tumefaciens was used extensively for transferring desired genetic materials to plants rapidly and effectively by the researchers to create transgenic plants. Recognition of the biology of Agrobacterium species and newly developed applications of their T-DNA systems has been a great step in plant biotechnology. This chapter provides the reader with extensive information on A. rhizogenes which is responsible for the development of hairy root disease in a wide range of dicotyledonous plants and its T-DNA system. This knowledge will be useful in improving utilization of crops and the formulation of new and up-graded transgenic based food products.

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Cited by 23 publications

References 23 publications

Alkamide production from hairy root cultures of Echinacea

Alkamide production from hairy root cultures of Echinacea

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

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