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Iqbal, Mohammad S.; Shad, Muhammad Aslam; Akhtar, Munir

doi:10.1023/a:1017940906191

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…It is, therefore, desirable to find rapid and more efficient methods for management of hyperbilirubinemia. An ever first study demonstrating degradation of bilirubin to biliverdin (an excretable product) in vitro by gamma irradiation was reported from our laboratory [12] followed by others [27, 28], which verified these results. This in vitro work could not be extended to in vivo experiments due to non-availability of suitable animal models.…”

Section: Introductionsupporting

confidence: 69%

Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit

2016

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Induction of hyperbilirubinemia in experimental rabbits by phenylhydrazine was optimized in terms of dose, dose interval and number of doses using response surface methodology. Central Composite Design was employed using five levels for each of the three input variables. Degree of hyperbilirubinemia was measured in terms of bilirubin level in serum of animals. A dose dependent significant elevation (P<0.05) of total serum bilirubin level was observed which was optimized by using eight factorial, six axial and six central points as suggested by experimental design. Optimum levels of phenylhydrazine dose, total number of doses and a dose interval to achieve maximum elevation (4.06 mg/dl−1) of total serum bilirubin were found to be 11.56 mg/kg−1 body weight, 8 and 24.65 h, respectively. The induction procedure was validated by performing five replicate experiments on a group of five animals which showed 3.56 ± 0.47 mg/kg−1 body weight elevation in total serum bilirubin level.

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Section: Introductionsupporting

confidence: 69%

Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit

2016

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Critical Evaluation of Gamma-Irradiated Serum Used as Feeder in the Culture and Demonstration of Putative Nanobacteria and Calcifying Nanoparticles

Martel

Young

2010

PLoS ONE

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The culture and demonstration of putative nanobacteria (NB) and calcifying nanoparticles (CNP) from human and animal tissues has relied primarily on the use of a culture supplement consisting of FBS that had been γ-irradiated at a dose of 30 kGy (γ-FBS). The use of γ-FBS is based on the assumption that this sterilized fluid has been rid entirely of any residual NB/CNP, while it continues to promote the slow growth in culture of NB/CNP from human/animal tissues. We show here that γ-irradiation (5–50 kGy) produces extensive dose-dependent serum protein breakdown as demonstrated through UV and visible light spectrophotometry, fluorometry, Fourier-transformed infrared spectroscopy, and gel electrophoresis. Yet, both γ-FBS and γ-irradiated human serum (γ-HS) produce NB/CNP in cell culture conditions that are morphologically and chemically indistinguishable from their normal serum counterparts. Contrary to earlier claims, γ-FBS does not enhance the formation of NB/CNP from several human body fluids (saliva, urine, ascites, and synovial fluid) tested. In the presence of additional precipitating ions, both γ-irradiated serum (FBS and HS) and γ-irradiated proteins (albumin and fetuin-A) retain the inherent dual NB inhibitory and seeding capabilities seen also with their untreated counterparts. By gel electrophoresis, the particles formed from both γ-FBS and γ-HS are seen to have assimilated into their scaffold the same smeared protein profiles found in the γ-irradiated sera. However, their protein compositions as identified by proteomics are virtually identical to those seen with particles formed from untreated serum. Moreover, particles derived from human fluids and cultured in the presence of γ-FBS contain proteins derived from both γ-FBS and the human fluid under investigation—a confusing and unprecedented scenario indicating that these particles harbor proteins from both the host tissue and the FBS used as feeder. Thus, the NB/CNP described in the literature clearly bear hybrid protein compositions belonging to different species. We conclude that there is no basis to justify the use of γ-FBS as a feeder for the growth and demonstration of NB/CNP or any NB-like particles in culture. Moreover, our results call into question the validity of the entire body of literature accumulated to date on NB and CNP.

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Cited by 2 publications

References 9 publications

Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit

Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit

Critical Evaluation of Gamma-Irradiated Serum Used as Feeder in the Culture and Demonstration of Putative Nanobacteria and Calcifying Nanoparticles

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