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Livingstone, D. Malcolm; Birch, Robert G.

doi:10.1023/a:1009658313170

Cited by 30 publications

(2 citation statements)

References 28 publications

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“…However, recent literature survey yielded a number of reports on the transformation and regeneration of different cultivars of groundnut across the globe. But, the Agrobacterium-mediated transformation efficiency in groundnut is genotype/cultivardependent [16,47]. The method described by Tiwari and Tuli [26] showed better results compared to others evaluated in the study in terms of transient transformation efficiency calculated after co-cultivation.…”

Section: Discussionmentioning

confidence: 99%

An Efficient Method of Agrobacterium-Mediated Genetic Transformation and Regeneration in Local Indian Cultivar of Groundnut (Arachis hypogaea) Using Grafting

Tiwari

Chaturvedi

Mishra

et al. 2014

Appl Biochem Biotechnol

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Groundnut (Arachis hypogaea L.) is an industrial crop used as a source of edible oil and nutrients. In this study, an efficient method of regeneration and Agrobacterium-mediated genetic transformation is reported for a local cultivar GG-20 using de-embryonated cotyledon explant. A high regeneration 52.69 ± 2.32 % was achieved by this method with 66.6 μM 6-benzylaminopurine (BAP), while the highest number of shoot buds per explant, 17.67 ± 3.51, was found with 20 μM BAP and 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D). The bacterial culture OD, acetosyringone and L-cysteine concentration were optimized as 1.8, 200 μM and 50 mg L(-1), respectively, in co-cultivation media. It was observed that the addition of 2,4-D in co-cultivation media induced accumulation of endogenous indole-3-acetic acid (IAA). The optimized protocol exhibited 85 % transformation efficiency followed by 14.65 ± 1.06 % regeneration, of which 3.82 ± 0.6 % explants were survived on hygromycin after selection. Finally, 14.58 ± 2.95 % shoots (regenerated on survived explants) were rooted on rooting media (RM3). In grafting method, regenerated shoots (after hygromycin selection) were grafted on the non-transformed stocks with 100 % survival and new leaves emerged in 3 weeks. The putative transgenic plants were then confirmed by PCR, Southern hybridization, reverse transcriptase PCR (RT-PCR) and β-glucuronidase (GUS) histochemical assay. The reported method is efficient and rapid and can also be applied to other crops which are recalcitrant and difficult in rooting.

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Section: Discussionmentioning

confidence: 99%

An Efficient Method of Agrobacterium-Mediated Genetic Transformation and Regeneration in Local Indian Cultivar of Groundnut (Arachis hypogaea) Using Grafting

Tiwari

Chaturvedi

Mishra

et al. 2014

Appl Biochem Biotechnol

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“…Till now, very few reporter gene systems were used in plant transformation. Of them, reporter genes like uidA/gusA (β-glucuronidase) [20], [41], [53], luc (luciferase) [55], and gfp (green fluorescent protein) [51] were substantially used in groundnut transformation studies, including other plant species as well. Use of gfp as reporter gene has its benefits throughout the process of non-destructive screening for positive transformed cells/calli in groundnut [56].…”

Section: Development Of Transgenic Plant: Basic Concept and Methodsmentioning

confidence: 99%

Transgenic approaches for genetic improvement in groundnut (Arachis hypogaea L.) against major biotic and abiotic stress factors

Gantait

Mondal

2018

Journal of Genetic Engineering and Biotechnology

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Cultivated groundnut ( Arachis hypogaea L.) is considered as one of the primary oilseed crops and a major fodder for cattle industry in most of the developing countries, owing to its rich source of protein. It is due to its geocarpic nature of growth that the overall yield performance of groundnut is hindered by several biotic and abiotic stress factors. Multidimensional attempts were undertaken to combat these factors by developing superior groundnut varieties, modified with integral mechanism of tolerance/resistance; however this approach proved to be futile, owing to inferior pod and kernel quality. As a superior alternative, biotechnological intervention like transformation of foreign genes, either directly (biolistic) or via Agrobacterium , significantly aided in the development of advanced groundnut genotypes equipped with integral resistance against stresses and enhanced yield attributing traits. Several genes triggered by biotic and abiotic stresses, were detected and some of them were cloned and transformed as major parts of transgenic programmes. Application of modern molecular biological techniques, in designing biotic and abiotic stress tolerant/resistant groundnut varieties that exhibited mechanisms of resistance, relied on the expression of specific genes associated to particular stress. The genetically transformed stress tolerant groundnut varieties possess the potential to be employed as donor parents in traditional breeding programmes for developing varieties that are resilient to fungal, bacterial, and viral diseases, as well as to draught and salinity. The present review emphasizes on the retrospect and prospect of genetic transformation tools, implemented for the enhancement of groundnut varieties against key biotic and abiotic stress factors.

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Plant Transformation Technology: Particle Bombardment

Twyman

Christou

2004

Handbook of Plant Biotechnology

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Stable gene transfer to plants can be achieved by two general strategies— Agrobacterium ‐mediated transformation and the direct introduction of DNA into the plant cell. Agrobacterium ‐mediated transformation is dependent on complex biological interactions between the bacterium and host plant, while direct DNA transfer is based on chemical or physical principles. Techniques included in this category, however, require particular cell types as transformation targets, and it is not always possible to regenerate plants from such cells. Only particle bombardment, the introduction of DNA into plants using high‐velocity microprojectiles, has the potential to transform any cell type from any plant, providing a truly universal transformation strategy. In this chapter, we discuss the principles of particle bombardment and variables that must be optimised for efficient transient and stable transformation. We also discuss the fate of exogenous DNA once it has entered the plant cell, and how this influences transgene expression. A literature survey is presented, listing all the plant species that have been transformed using this method, up to the end of the year 2001.

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Untitled

Cited by 30 publications

References 28 publications

An Efficient Method of Agrobacterium-Mediated Genetic Transformation and Regeneration in Local Indian Cultivar of Groundnut (Arachis hypogaea) Using Grafting

An Efficient Method of Agrobacterium-Mediated Genetic Transformation and Regeneration in Local Indian Cultivar of Groundnut (Arachis hypogaea) Using Grafting

Transgenic approaches for genetic improvement in groundnut (Arachis hypogaea L.) against major biotic and abiotic stress factors

Plant Transformation Technology: Particle Bombardment

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