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Ackerman, Paige A.; Forsyth, Rob B.; Mazur, Carl F.; Iwama, George K.

doi:10.1023/a:1011107610971

Cited by 112 publications

(8 citation statements)

References 37 publications

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“…In particular, our work determined the effects of cortisol-mediated stress on the transcriptional response of rainbow trout, discarding the large number of factors associated with the stress response (44). The high cortisol plasmatic levels reached in our assay have been previously observed in similar protocols of exogenous cortisol administration in rainbow trout (1,3). In this context, we propose that the skeletal muscle transcriptional response observed in our in vivo assay would be related to an intense acute cortisol-mediated stress in this species.…”

Section: Discussionmentioning

confidence: 92%

Early transcriptomic responses associated with the membrane-initiated action of cortisol in the skeletal muscle of rainbow trout (Oncorhynchus mykiss)

Aedo

Zuloaga

Bastías-Molina

et al. 2019

Physiological Genomics

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Cortisol is a critical neuroendocrine regulator of the stress response in fish. Cortisol practically affects all tissues by interacting with an intracellular receptor and modulating target gene expression. However, cortisol also interacts with components of the plasma membrane in a nongenomic process that activates rapid signaling. Until now, the implication of this novel cortisol signaling for the global transcriptional response has not been explored. In the present work, we evaluated the effects of the membrane-initiated actions of cortisol on the in vivo transcriptome of rainbow trout ( Oncorhynchus mykiss) skeletal muscle. RNA-Seq analyses were performed to examine the transcriptomic changes in rainbow trout stimulated by physiological concentrations of cortisol and cortisol coupled with bovine serum albumin (cortisol-BSA), a membrane-impermeable analog of cortisol. A total of 660 million paired-ends reads were generated. Reads mapped onto the reference genome revealed that 1,737; 897; and 1,012 transcripts were differentially expressed after 1, 3, and 9 h of cortisol-BSA treatment, respectively. Gene Ontology analysis showed that this novel action of cortisol modulates several biological processes, such as mRNA processing, ubiquitin-dependent protein catabolic processes, and transcription regulation. In addition, a KEGG analysis revealed that focal adhesion was the main signaling pathway that was upregulated at all the times tested. Taking these results together, we propose that the membrane-initiated cortisol action contributes significantly in the regulation of stress-mediated gene expression.

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Section: Discussionmentioning

confidence: 92%

Early transcriptomic responses associated with the membrane-initiated action of cortisol in the skeletal muscle of rainbow trout (Oncorhynchus mykiss)

Aedo

Zuloaga

Bastías-Molina

et al. 2019

Physiological Genomics

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“…A final explanation is that the F. parvipinnis naturally exhibit a negative relationship between handling time and cortisol release rates (K. L. Weinersmith & R. L. Earley, unpubl. data), and EUHA becomes increasingly able to manipulate the fish's cortisol response as the density of the parasite increases. While teleosts typically show no cortisol response to handling (Wong et al, 2008) or increased cortisol in response to handling (Ackerman et al, 2000), the predictor estimate for handling in F. parvipinnis was negative (Table SIII, Supporting Information). The c.i.…”

Section: H O R M O N E R E L E a S E R At E S A N D Pa R A S I T E Smentioning

confidence: 99%

The relationship between handling time and cortisol release rates changes as a function of brain parasite densities in California killifishFundulus parvipinnis

Weinersmith

Hanninen

Sih

et al. 2016

Journal of Fish Biology

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This study validated a technique for non-invasive hormone measurements in California killifish Fundulus parvipinnis, and looked for associations between cortisol (a stress hormone) and 11-ketotestosterone (KT, an androgen) release rates and the density or intensity of the trematode parasites Euhaplorchis californiensis (EUHA) and Renicola buchanani (RENB) in wild-caught, naturally infected F. parvipinnis. In experiment 1, F. parvipinnis were exposed to an acute stressor by lowering water levels to dorsal-fin height and repeatedly handling the fish over the course of an hour. Neither parasite was found to influence cortisol release rates in response to this acute stressor. In experiment 2, different F. parvipinnis were exposed on four consecutive days to the procedure for collecting water-borne hormone levels and release rates of 11-KT and cortisol were quantified. This design examined whether F. parvipinnis perceived the water-borne collection procedure to be a stressor, while also exploring how parasites influenced hormone release rates under conditions less stressful than those in experiment 1. No association was found between RENB and hormone release rates, or between EUHA and 11-KT release rates. The interaction between EUHA density and handling time, however, was an important predictor of cortisol release rates. The relationship between handling time and cortisol release rates was negative for F. parvipinnis harbouring low or intermediate density infections, and became positive for fish harbouring high densities of EUHA.

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“…In fish, the intracellular induction of Hsps, in particular Hsp70, has been well characterized in response to a plethora of stimuli (Iwama et al 1999, Basu et al 2002, Vijayan et al 2010, Deane & Woo 2011. Also, studies have shown that cortisol, the primary circulating corticosteroid in teleost, modulates target tissue Hsp70 expression in fishes, thereby linking the cellular stress response with the organismal stress response (Ackerman et al 2000, Basu et al 2001, Boone & Vijayan 2002, De Boeck et al 2003. Recent studies suggest that Hsp70 may also be released extracellularly in fishes, but the secretory pathways are far from clear (Illiev et al 2010, Zhang et al 2011.…”

Section: :2mentioning

confidence: 99%

Plasma exosomes are enriched in Hsp70 and modulated by stress and cortisol in rainbow trout

Faught

Henrickson

Vijayan

2017

Journal of Endocrinology

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Exosomes are endosomally derived vesicles that are secreted from cells and contain a suite of molecules, including proteins and nucleic acids. Recent studies suggest the possibility that exosomes in circulation may be affecting recipient target cell function, but the modes of action are unclear. Here, we tested the hypothesis that exosomes are in circulation in fish plasma and that these vesicles are enriched with heat shock protein 70 (Hsp70). Exosomes were isolated from rainbow trout (Oncorhynchus mykiss) plasma using differential centrifugation, and their presence was confirmed by transmission electron microscopy and the exosomal marker acetylcholinesterase. Plasma exosomes were enriched with Hsp70, and this stress protein was transiently elevated in trout plasma in response to a heat shock in vivo Using trout hepatocytes in primary culture, we tested whether stress levels of cortisol, the principle corticosteroid in teleosts, regulates exosomal Hsp70 content. As expected, a 1-h heat shock (+15°C above ambient) increased Hsp70 expression in hepatocytes, and this led to higher Hsp70 enrichment in exosomes over a 24-h period. However, cortisol treatment significantly reduced the expression of Hsp70 in exosomes released from either unstressed or heat-shocked hepatocytes. This cortisol-mediated suppression was not specific to Hsp70 as beta-actin expression was also reduced in exosomes released from hepatocytes treated with the steroid. Our results suggest that circulating Hsp70 is released from target tissues via exosomes, and their release is modulated by stress and cortisol. Overall, we propose a novel role for extracellular vesicular transport of Hsp70 in the organismal stress response.

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Cited by 112 publications

References 37 publications

Early transcriptomic responses associated with the membrane-initiated action of cortisol in the skeletal muscle of rainbow trout (Oncorhynchus mykiss)

Early transcriptomic responses associated with the membrane-initiated action of cortisol in the skeletal muscle of rainbow trout (Oncorhynchus mykiss)

The relationship between handling time and cortisol release rates changes as a function of brain parasite densities in California killifishFundulus parvipinnis

Plasma exosomes are enriched in Hsp70 and modulated by stress and cortisol in rainbow trout

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