Untitled

Floß, Barbara; Igloi, Gabor L.; Cassier‐Chauvat, Corinne; Mühlenhoff, Ulrich

doi:10.1023/a:1005823620291

Cited by 17 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The petF gene encoding Fd (SynPcc7942_1499) was amplified from genomic DNA of PCC7942 by PCR, cloned into the vector pET-28a(+) at the restriction sites of Nde I and Xho I, and overexpressed in E. coli BL21(DE3) in M9 medium under IPTG induction at 30°C [35]. Overexpressed Fd was purified on Nickel column.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system

Zhang

Lü

2013

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundBiosynthesis of fatty alk(a/e)ne in cyanobacteria has been considered as a potential basis for the sunlight-driven and carbon-neutral bioprocess producing advanced solar biofuels. Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in that pathway. The heterologous or chemical reducing systems were generally used in in vitro ADO activity assay. The cognate electron transfer system from cyanobacteria to support ADO activity is still unknown.ResultsWe identified the potential endogenous reducing system including ferredoxin (Fd) and ferredoxin-NADP+ reductase (FNR) to support ADO activity in Synechococcus elongatus PCC7942. ADO (Synpcc7942_1593), FNR (SynPcc7942_0978), and Fd (SynPcc7942_1499) from PCC7942 were cloned, overexpressed, purified, and characterized. ADO activity was successfully supported with the endogenous electron transfer system, which worked more effectively than the heterologous and chemical ones. The results of the hybrid Fd/FNR reducing systems demonstrated that ADO was selective against Fd. And it was observed that the cognate reducing system produced less H2O2 than the heterologous one by 33% during ADO-catalyzed reactions. Importantly, kcat value of ADO 1593 using the homologous Fd/FNR electron transfer system is 3.7-fold higher than the chemical one.ConclusionsThe cognate electron transfer system from cyanobacteria to support ADO activity was identified and characterized. For the first time, ADO was functionally in vitro reconstituted with the endogenous reducing system from cyanobacteria, which supported greater activity than the surrogate and chemical ones, and produced less H2O2 than the heterologous one. The identified Fd/FNR electron transfer system will be potentially useful for improving ADO activity and further enhancing the biosynthetic efficiency of hydrocarbon biofuels in cyanobacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Protein expression was carried out at 30°C in M9ZB media supplemented with 50 μg/mL kanamycin and 50 μM FeCl 3 [35]. The cultures were induced with 0.2 mM IPTG when OD 600 reached 0.7, and were shaken at 16°C for 48 hr.…”

Section: Methodsmentioning

confidence: 99%

Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system

Zhang

Lü

2013

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…Moreover, leaf color mutants also induced differential expression of photosynthesisrelated genes, including coding for chloroplast proteins and other regulatory proteins or enzymes controlling genes, such as PSII reaction center D1 and D2 protein (PsbA and PsbD) [24], oxygen-evolving enhancer protein (PsbO and PsbP) [25], plastocyanin (PetE) [26], ferredoxin (PetF) [27], ferredoxin-NADP + reductase (PetH) [28], Rubisco (rbcL) [29], and a series of F-type H + /Na + -transporting ATPase subunit genes in different plant species. These genes are necessary for normal growth and development as well as responses to environmental changes in plants, and could be utilized for characterizing photosynthesissensitive colored leaf genotypes.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Molecular Analysis Reveals the Differences of Photosynthesis between Colored and Green Leaf Poplars

Wang

Cheng

et al. 2021

IJMS

View full text Add to dashboard Cite

Leaf coloration changes evoke different photosynthetic responses among different poplar cultivars. The aim of this study is to investigate the photosynthetic difference between a red leaf cultivar (ZHP) and a green leaf (L2025) cultivar of Populus deltoides. In this study, ‘ZHP’ exhibited wide ranges and huge potential for absorption and utilization of light energy and CO2 concentration which were similar to those in ‘L2025’ and even showed a stronger absorption for weak light. However, with the increasing light intensity and CO2 concentration, the photosynthetic capacity in both ‘L2025’ and ‘ZHP’ was gradually restricted, and the net photosynthetic rate (Pn) in ‘ZHP’ was significantly lower than that in ‘L2025’under high light or high CO2 conditions, which was mainly attributed to stomatal regulation and different photosynthetic efficiency (including the light energy utilization efficiency and photosynthetic CO2 assimilation efficiency) in these two poplars. Moreover, the higher anthocyanin content in ‘ZHP’ than that in ‘L2025’ was considered to be closely related to the decreased photosynthetic efficiency in ‘ZHP’. According to the results from the JIP-test, the capture efficiency of the reaction center for light energy in ‘L2025’ was significantly higher than that in ‘ZHP’. Interestingly, the higher levels of light quantum caused relatively higher accumulation of QA- in ‘L2025’, which blocked the electron transport and weakened the photosystem II (PSII) performance as compared with ‘ZHP’; however, the decreased capture of light quantum also could not promote the utilization of light energy, which was the key to the low photosynthetic efficiency in ‘ZHP’. The differential expressions of a series of photosynthesis-related genes further promoted these specific photosynthetic processes between ‘L2025’ and ‘ZHP’.

show abstract

“…The petC gene encodes an iron-sulfur protein, which is an electron carrier and plays important roles in vital activities (Zusman et al, 2004). Overexpression of petF could distribute photosynthetic electrons to a variety of essential metabolic pathways and also increase the efficiency of scavenging reactive oxygen species, thereby alleviating damage to organelles (Floß et al, 1997;Lin et al, 2013). The petH gene is a flavoenzyme that catalyses the terminal step in PSI-associated electron transport, converting NADP + and H + into NADPH (Martínez-Júlvez et al, 1996).…”

Section: Up-regulation Of Genes Involved In Photosynthetic Electron Tmentioning

confidence: 99%

Enhancing Photosynthetic Characterization and Biomass Productivity of Nannochloropsis Oceanica by Nuclear Radiation

Cheng

Wang

et al. 2020

Front. Energy Res.

View full text Add to dashboard Cite

Microalgae with a high growth rate and a carbon fixation rate present a promising potential to produce diverse renewable energy products and reduce greenhouse gas emissions. However, some microalgal species may have limited light-use efficiency and specific growth rate. To alleviate these issues, Nannochloropsis oceanica was subjected to 137 Cs-γ radiation to obtain the desired mutant with enhanced light-use efficiency and increased biomass productivity. The N. oceanica mutant ZJU700 showed a 26.7% increase in biomass productivity after nuclear irradiation at a 700 GY dosage. It was found that the mutant had a 30.2% higher oxygen evolution rate than the wild type cells. High-throughput transcriptome sequencing showed that expression of photosynthetic related genes in the mutant were much higher than in wild type cells. Expression of the psbO gene increased by 455% in mutant ZJU700, contributing to an increased oxygen evolution rate by splitting water. Three up-regulated genes, namely petC, petF, and petH, resulted in enhanced electron transport during the photoreaction process. Up-regulation of many genes involved in the Calvin cycle indicated that CO 2 fixation rate was likely to be increased to produce more carbohydrates in mutant, thereby contributing to the increased biomass productivity in mutant ZJU700.

show abstract

Untitled

Cited by 17 publications

References 35 publications

Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system

Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system

Physiological and Molecular Analysis Reveals the Differences of Photosynthesis between Colored and Green Leaf Poplars

Enhancing Photosynthetic Characterization and Biomass Productivity of Nannochloropsis Oceanica by Nuclear Radiation

Contact Info

Product

Resources

About