Untitled

Resca, R.; Basaglia, Marina; Poggiolini, S.; Vian, P.; Bardin, Sylvie D.; Walsh, U. F.; Barreiros, C. M.; O’Gara, Fergal; Nuti, Marco; Casella, Sergio; Peruch, U.

doi:10.1023/a:1010310825363

Cited by 26 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The development of an effective strain that can tolerate the environmental adversities and securing of the essential regulatory approval are primary obligations for the successful use of a GM inoculant as biocontrol agents [17]. Resca et al [18] genetically modified the strains of P. fluorescens F113Rif (pCU8.3) and P. fluorescens F113Rif (pCUP9) to increase phenazine1 carboxylate (Phl) production for biocontrol efficacy against Polymyxa betae on sugar beet. An endochitinase-encoding gene was cloned from the T. viride.…”

Section: Genetic Manipulation Requires the Development Of Vector-medimentioning

confidence: 99%

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

Mirmajlessi¹,

Mostafavi²,

Loit³

et al. 2019

Use of Gamma Radiation Techniques in Peaceful Applications

View full text Add to dashboard Cite

Biological control is a potential nonchemical method to manage plant pathogens by beneficial microorganisms. To improve antagonistic potential of biocontrol agents, mutation by radiations, chemicals, and genetic manipulations has been used. Genetic techniques and ionizing radiation containing direct or indirect emissions play the greatest role for selection of useful microorganisms to enhance the efficiency of biological systems. Indeed, genetic engineering has a main role in increasing antimicrobial metabolites, host colonization ability, and endurance in micro-ecosystem. Genetic improvement can be achieved by protoplast fusion, genetic modification (GM), and chemical (genotoxic agents) and physical mutations. However, ultraviolet light and ionizing radiations can induce modifications in the genome of an organism. Irradiation, particularly gamma rays, is also applied for controlling postharvest diseases. Indeed, irradiation cannot completely eliminate pathogens, but it might result in cell injury and directly damage the chromosomal DNA of a living cell. This technology has been used for many reasons including disinfestation of foods, reducing foodborne pathogens, and extending shelf life many fruits, vegetables, and nuts. In the current review, we discuss advances in the radiation and molecular genetic techniques with the aim to improve antagonistic potential of microorganisms as it is applied to the suppression of plant pathogens.

show abstract

Section: Genetic Manipulation Requires the Development Of Vector-medimentioning

confidence: 99%

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

Mirmajlessi¹,

Mostafavi²,

Loit³

et al. 2019

Use of Gamma Radiation Techniques in Peaceful Applications

View full text Add to dashboard Cite

show abstract

“…These factors include the ability of the microbial inoculant to survive in the rhizosphere and compete with the indigenous microbial populations as well as the ability to produce effective concentrations of the antibiotic metabolites at the time and site of disease [14]. Phl is a significant secondary metabolite with a broad spectrum of antifungal, antirhizomania, antimicrobial and antihelminthic activities [15,[17][18][19][20][21][22][23][24]. Genetic and molecular analysis has established a link between the production of this polyketide and the biocontrol efficacy of P. fluorescens F113 [15,16].…”

Section: Wcs417rmentioning

confidence: 99%

Pseudomonas–Plant Interactions

et al. 2008

Self Cite

View full text Add to dashboard Cite

Pseudomonas is a diverse genus containing a large number of species with a variety of catabolic and metabolic abilities. This diversity allows for the colonization of an array of environmental niches and interactions with a wide range of eukaryotic hosts as saprotrophs, endophytes, commensals, plant pathogens and opportunistic human pathogens [1][2][3][4][5]. Pseudomonas species are of great interest to researchers in the fields of plant, soil and human-associated microbiology, and as such the genomic sequences of a range of Pseudomonas strains have been determined or are currently in progress (Table 13.1). Pseudomonas-plant interactions are found ubiquitously in nature and encompass a growing number of plant species, many of which are important to commercial horticulture. These interactions fall into two general groups: those that are beneficial and those that are detrimental to the host plants health (Figure 13.1). Pseudomonas sp. which successfully interact with plant hosts ultimately gain an advantage over the competing microorganisms in the immediate environment. This is usually achieved by occupying an exclusive niche provided by the plants surfaces and obtaining growth substrates exuded from the plant roots or released by disease-related breakdown of plant cells in the case of pathogenic interactions. The development of such interactions between Pseudomonas and host plants usually involves avoidance, subversion and sometimes even stimulation of the host plants defenses. In return the plant host can respond to the presence of the Pseudomonas sp. by modulating defense mechanisms, and engaging in signaling between itself and the infecting bacterium in an attempt to further promote beneficial interactions or suppress pathogenic ones. The molecular game of cat and mouse between the partners, with each attempting to benefit from the other, has evolved over millions of years and provides us now with a vast array of Pseudomonas-plant interaction mechanisms to unravel. Indeed, genetic and physiological similarities Pseudomonas. Model Organism, Pathogen, Cell Factory. Edited by Bernd H.A. Rehm

show abstract

“…Biological methods using bacterial and fungal agents to prevent the colonization of sugar beet roots by P. betae have also been used. Results showed that the bacterium Pseudomonas fluorescence, which was used as a seed treatment, cannot control the disease (Resca et al., 2001 ). However, various types of Trichoderma species can reduce rhizomania outbreak between 21 and 68% (Jakubikova et al., 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of rhizomania infection on sugar beet quality in multi‐year field assessment

Fasahat,

Aghaeezadeh,

Taleghani

et al. 2024

Food Science & Nutrition

View full text Add to dashboard Cite

Rhizomania is one of the most destructive and damaging sugar beet diseases that has spread in different regions of Iran. In order to evaluate the genotypic, environmental, and genotype by environmental variability of sugar beet genotypes under rhizomania infection, variance components were estimated from the trial series in 7 years. Required data, such as yield and quality parameters, were collected from value for cultivation and use trials. Results of analysis of variance showed that the environment was the source that explained most of the variability, except for amino‐N and alkalinity. Quality traits were also influenced by the environment × cultivar interaction, so that 4.8% (white sugar content) to 46.1% (alkalinity) variance was observed. In contrast, genetic variation was much lower, between 1.2% (potassium) and 27.4% (amino‐N). A strong and negative correlation was found between root yield, sugar yield, and white sugar content with the disease index, which obviously illustrates the negative impact of the rhizomania on root weight and as a consequence on the dependent traits. The cluster analysis of the cultivars based on the quantitative and qualitative traits and the disease index showed that the range of variation in traits, such as the disease index, varied from 6.25 for the susceptible cultivar to 1.25 for the resistant one. This indicates the existence of sufficient genetic diversity among cultivars in terms of this trait. High impurity accumulation was observed in Shiraz region compared with Mashhad. In conclusion, it is observed that rhizomania has a significant effect on the impurity concentration in the root, especially sodium, potassium, and amino‐N. This is very important in the sugar industry because sugar extraction depends on the concentration of these impurities, in addition to the sugar content of each cultivar.

show abstract

Untitled

Cited by 26 publications

References 24 publications

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

Pseudomonas–Plant Interactions

Evaluation of rhizomania infection on sugar beet quality in multi‐year field assessment

Contact Info

Product

Resources

About