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Hussey, Patrick J.; Hawkins, Timothy J.; Igarashi, Hisako; Kaloriti, Despina; Smertenko, Andrei

doi:10.1023/a:1021236307508

Cited by 129 publications

(26 citation statements)

References 50 publications

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“…Three cDNA sequences that encode three highly similar polypeptides, NtMAP65-1a, NtMAP65-1b, and NtMAP65-1c, have been isolated from tobacco (Smertenko et al 2000). When the amino acid sequences of these proteins were compared with those of the nine Arabidopsis MAP65 proteins, the three tobacco MAP65 members were found to share high degrees of identity with AtMAP65-1 and AtMAP65-2 (Hussey et al 2002). Biochemical data suggest that, although they probably all have MT-bundling activity, these highly-similar MAP65s exert different effects on MT dynamics and stability in vitro.…”

Section: Characteristics Of the Effects Of Atmap65-2 On Mtsmentioning

confidence: 99%

“…MAP65 family (Jiang and Sonobe 1993;Chan et al 1996Chan et al , 1999Hussey et al 2002). In tobacco, the MAP65 protein family constitutes three or four proteins (Jiang and Sonobe 1993;Smertenko et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Nine members of the MAP65 family have been identified in Arabidopsis, and their shared amino acid identities range between 22 and 82% (Hussey et al 2002). The subcellular localization of some members of the AtMAP65 family has been investigated by immunostaining of suspensioncultured Arabidopsis cells and by expressing GFP-tagged AtMAP65 proteins in suspension-cultured tobacco cells.…”

Section: Introductionmentioning

confidence: 99%

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Arabidopsis microtubule-associated protein AtMAP65-2 acts as a microtubule stabilizer

Zeng

Liu

et al. 2008

Plant Mol Biol

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Nine genes that encode proteins of the MAP65 family have been identified in the Arabidopsis thaliana genome. In this study, we reported that AtMAP65-2, a member of the AtMAP65 family, could strongly stabilize microtubules (MTs). Bacterially-expressed AtMAP65-2 fusion proteins induced the formation of large MT bundles in vitro. Although AtMAP65-2 showed little effect on MT assembly or nucleation, AtMAP65-2 greatly stabilized MTs that were subjected to low-temperature treatment in vitro. Analyses of truncated versions of AtMAP65-2 indicated that the region that encompassed amino acids 495-578, which formed a flexible extended loop, played a crucial role in the stabilization of MTs. Analysis of suspension-cultured Arabidopsis cells that expressed the AtMAP65-2-GFP fusion protein showed that AtMAP65-2 co-localized with MTs throughout the cell cycle. Cortical MTs that were decorated with AtMAP65-2-GFP were more resistant to the MT-disrupting drug propyzamide and to ice treatment in vivo. The results of this study demonstrate that AtMAP65-2 strongly stabilizes MTs and is involved in the regulation of MT organization and dynamics.

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Section: Characteristics Of the Effects Of Atmap65-2 On Mtsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Arabidopsis microtubule-associated protein AtMAP65-2 acts as a microtubule stabilizer

Zeng

Liu

et al. 2008

Plant Mol Biol

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“…Subsequently, MAP65 proteins were identified respectively in other plants. Arabidopsis has nine MAP65 proteins with predicted molecular masses between 54 and 80kDa (Hussey et al , 2002). The rice genome encodes 11 members of the MAP65 family (Guo et al , 2009).…”

Section: Introductionmentioning

confidence: 99%

MAP65-1a positively regulates H2O2 amplification and enhances brassinosteroid-induced antioxidant defence in maize

Zhu

Zuo

et al. 2013

Journal of Experimental Botany

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Brassinosteroid (BR)-induced antioxidant defence has been shown to enhance stress tolerance. In this study, the role of the maize 65kDa microtubule-associated protein (MAP65), ZmMAP65-1a, in BR-induced antioxidant defence was investigated. Treatment with BR increased the expression of ZmMAP65-1a in maize (Zea mays) leaves and mesophyll protoplasts. Transient expression and RNA interference silencing of ZmMAP65-1a in mesophyll protoplasts further revealed that ZmMAP65-1a is required for the BR-induced increase in expression and activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX). Both exogenous and BR-induced endogenous H2O2 increased the expression of ZmMAP65-1a. Conversely, transient expression of ZmMAP65-1a in maize mesophyll protoplasts enhanced BR-induced H2O2 accumulation, while transient silencing of ZmMAP65-1a blocked the BR-induced expression of NADPH oxidase genes and inhibited BR-induced H2O2 accumulation. Inhibiting the activity and gene expression of ZmMPK5 significantly prevented the BR-induced expression of ZmMAP65-1a. Likewise, transient expression of ZmMPK5 enhanced BR-induced activities of the antioxidant defence enzymes SOD and APX in a ZmMAP65- 1a-dependent manner. ZmMPK5 directly interacted with ZmMAP65-1a in vivo and phosphorylated ZmMAP65-1a in vitro. These results suggest that BR-induced antioxidant defence in maize operates through the interaction of ZmMPK5 with ZmMAP65-1a. Furthermore, ZmMAP65-1a functions in H2O2 self-propagation via regulation of the expression of NADPH oxidase genes in BR signalling.

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“…Isotype-specific expression patterns may vary in time and space, determining profiles of expression that can be unique or overlapping among different isotypes. In turn, this changes MT composition and properties with influence on MT dynamics or binding with specific Microtubule Associated Proteins (MAPs; Hussey et al 2002). Many mechanisms influence MT composition.…”

Section: Introductionmentioning

confidence: 99%

In trangenic rice, α- and β-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression

et al. 2008

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The genomic upstream sequence of the rice tubulin gene OsTub6 has been cloned, sequenced and characterized. The 5'UTR sequence is interrupted by a 446 bp long leader intron. This feature is shared with two other rice beta-tubulin genes (OsTub4 and OsTub1) that, together with OsTub6, group in the same clade in the evolutionary phylogenetic tree of plant beta-tubulins. Similarly to OsTub4, the leader intron of OsTub6 is capable of sustaining intron mediated enhancement (IME) of gene expression, in transient expression assays. A general picture is drawn for three rice alpha-tubulin and two rice beta-tubulin genes in which the first intron of the coding sequence for the formers and the intron present in the 5'UTR for the latters, are important elements for controlling gene expression. We used OsTua2:GUS, OsTua3:GUS, OsTub4:GUS and OsTub6:GUS chimeric constructs to investigate the in vivo pattern of beta-glucuronidase (GUS) expression in transgenic rice plants. The influence of the regulatory introns on expression patterns was evaluated for two of them, OsTua2 and OsTub4. We have thus characterized distinct patterns of expression attributable to each tubulin isotype and we have shown that the presence of the regulatory intron can greatly influence both the amount and the actual site of expression. We propose the term Intron Dependent Spatial Expression (IDSE) to highlight this latter effect.

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Untitled

Cited by 129 publications

References 50 publications

Arabidopsis microtubule-associated protein AtMAP65-2 acts as a microtubule stabilizer

Arabidopsis microtubule-associated protein AtMAP65-2 acts as a microtubule stabilizer

MAP65-1a positively regulates H2O2 amplification and enhances brassinosteroid-induced antioxidant defence in maize

In trangenic rice, α- and β-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression

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