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Yablochkova, E. N.

doi:10.1023/a:1023523510401

Cited by 12 publications

(1 citation statement)

References 3 publications

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“…Wilkins et al [32] reported maximum xylose consumption of 13.61 g l ¡1 in 96 h with ethanol productivity of 0.02 g l ¡1 h ¡1 and xylitol productivity of 0.08 g l ¡1 h ¡1 using Kluyveromyces marxianus IMB4 at 40°C. In another study, Yablochkova et al [33] reported ethanol productivity of 0.315 g l ¡1 h ¡1 on glucose and ethanol productivity of 0.0033 g l ¡1 h ¡1 and xylitol productivity of 0.105 g l ¡1 h ¡1 on xylose by Kluyveromyces marxianus. No signiWcant change was observed during fermentation in the cell-mass concentration, which means the ethanol formation is non-growth associated when using Kluyveromyces sp.…”

Section: Discussionmentioning

confidence: 96%

Ethanol and xylitol production from glucose and xylose at high temperature by Kluyveromyces sp. IIPE453

Kumar

Singh

Mishra

et al. 2009

J Ind Microbiol Biotechnol

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A yeast strain Kluyveromyces sp. IIPE453 (MTCC 5314), isolated from soil samples collected from dumping sites of crushed sugarcane bagasse in Sugar Mill, showed growth and fermentation efficiency at high temperatures ranging from 45 degrees C to 50 degrees C. The yeast strain was able to use a wide range of substrates, such as glucose, xylose, mannose, galactose, arabinose, sucrose, and cellobiose, either for growth or fermentation to ethanol. The strain also showed xylitol production from xylose. In batch fermentation, the strain showed maximum ethanol concentration of 82 +/- 0.5 g l(-1) (10.4% v/v) on initial glucose concentration of 200 g l(-1), and ethanol concentration of 1.75 +/- 0.05 g l(-1) as well as xylitol concentration of 11.5 +/- 0.4 g l(-1) on initial xylose concentration of 20 g l(-1) at 50 degrees C. The strain was capable of simultaneously using glucose and xylose in a mixture of glucose concentration of 75 g l(-1) and xylose concentration of 25 g l(-1), achieving maximum ethanol concentration of 38 +/- 0.5 g l(-1) and xylitol concentration of 14.5 +/- 0.2 g l(-1) in batch fermentation. High stability of the strain was observed in a continuous fermentation by feeding the mixture of glucose concentration of 75 g l(-1) and xylose concentration of 25 g l(-1) by recycling the cells, achieving maximum ethanol concentration of 30.8 +/- 6.2 g l(-1) and xylitol concentration of 7.35 +/- 3.3 g l(-1) with ethanol productivity of 3.1 +/- 0.6 g l(-1) h(-1) and xylitol productivity of 0.75 +/- 0.35 g l(-1) h(-1), respectively.

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Section: Discussionmentioning

confidence: 96%

Ethanol and xylitol production from glucose and xylose at high temperature by Kluyveromyces sp. IIPE453

Kumar

Singh

Mishra

et al. 2009

J Ind Microbiol Biotechnol

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d‐Xylose consumption by nonrecombinant Saccharomyces cerevisiae: A review

Patiño

Ortiz

Velásquez

et al. 2019

Yeast

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Xylose is the second most abundant sugar in nature. Its efficient fermentation has been considered as a critical factor for a feasible conversion of renewable biomass resources into biofuels and other chemicals. The yeast Saccharomyces cerevisiae is of exceptional industrial importance due to its excellent capability to ferment sugars. However, although S. cerevisiae is able to ferment xylulose, it is considered unable to metabolize xylose, and thus, a lot of research has been directed to engineer this yeast with heterologous genes to allow xylose consumption and fermentation. The analysis of the natural genetic diversity of this yeast has also revealed some nonrecombinant S. cerevisiae strains that consume or even grow (modestly) on xylose. The genome of this yeast has all the genes required for xylose transport and metabolism through the xylose reductase, xylitol dehydrogenase, and xylulokinase pathway, but there seems to be problems in their kinetic properties and/or required expression. Self‐cloning industrial S. cerevisiae strains overexpressing some of the endogenous genes have shown interesting results, and new strategies and approaches designed to improve these S. cerevisiae strains for ethanol production from xylose will also be presented in this review.

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Cashew apple bagasse as a source of sugars for ethanol production by Kluyveromyces marxianus CE025

Rocha

Rodrigues

Melo

et al. 2010

J Ind Microbiol Biotechnol

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The potential of cashew apple bagasse as a source of sugars for ethanol production by Kluyveromyces marxianus CE025 was evaluated in this work. This strain was preliminarily cultivated in a synthetic medium containing glucose and xylose and was able to produce ethanol and xylitol at pH 4.5. Next, cashew apple bagasse hydrolysate (CABH) was prepared by a diluted sulfuric acid pretreatment and used as fermentation media. This hydrolysate is rich in glucose, xylose, and arabinose and contains traces of formic acid and acetic acid. In batch fermentations of CABH at pH 4.5, the strain produced only ethanol. The effects of temperature on the kinetic parameters of ethanol fermentation by K. marxianus CE025 using CABH were also evaluated. Maximum specific growth rate (μ(max)), overall yields of ethanol based on glucose consumption [Formula: see text] and based on glucose + xylose consumption (Y ( P/S )), overall yield of ethanol based on biomass (Y ( P/X )), and ethanol productivity (P (E)) were determined as a function of temperature. Best results of ethanol production were achieved at 30°C, which is also quite close to the optimum temperature for the formation of biomass. The process yielded 12.36 ± 0.06 g l(-1) of ethanol with a volumetric production rate of 0.257 ± 0.002 g l(-1) h(-1) and an ethanol yield of 0.417 ± 0.003 g g(-1) glucose.

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Cited by 12 publications

References 3 publications

Ethanol and xylitol production from glucose and xylose at high temperature by Kluyveromyces sp. IIPE453

Ethanol and xylitol production from glucose and xylose at high temperature by Kluyveromyces sp. IIPE453

d‐Xylose consumption by nonrecombinant Saccharomyces cerevisiae: A review

Cashew apple bagasse as a source of sugars for ethanol production by Kluyveromyces marxianus CE025

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