The amygdalae play an important role in the evaluation and processing of emotionally salient visual stimuli. However, individual differences in personality traits, such as Harm Avoidance (HA), have been reported to influence emotional amygdalae responses. To trigger strong approach and withdrawal-related emotions in 'never depressed' young female subjects under fMRI, we presented them with blocks of happy 'healthy' baby faces and baby faces disfigured by severe dermatological conditions and we integrated the temperament dimension HA into our analysis. No other instructions were given than to watch the images attentively. Only in withdrawal-related emotional experience, we observed a negative correlation between HA and left amygdala activity, suggesting that during passive viewing females scoring higher on HA 'avoid' images with highly aversive content. When investigating the amygdala's emotional role in passive viewing paradigms, personality features such as HA should be taken into account.Keywords: Approach and withdrawal-related emotions, Amygdala, fMRI, Healthy Females, Harm Avoidance 3 A number of studies, using a variety of task instructions, have investigated the role of the amygdalae in the evaluation of social cues and the processing of emotional stimuli such as 'emotional' facial expressions and gender, age, and differences in personality features have been reported to influence amygdala activity [1,40]. When introducing individual information such as personality characteristics as covariate in the analysis of 'emotional' fMRI paradigms in healthy individuals, predominantly left sided amygdala activation has been reported [11]. Increased (left-sided) amygdala activation has also been reported in 'anxiety-prone' individuals (non-clinical subjects displaying high levels of anxiety-related traits) during the processing of emotion [5]. In addition, 'emotional' brain imaging research has already demonstrated the importance of the temperament dimension Harm Avoidance (HA) [29], extracted out of the Temperament and Character Inventory (TCI [12]). High scorers on HA show a tendency to respond more intensely to aversive stimuli compared to low HA scorers [24]. Subjects who score high on HA describe themselves as fearful, pessimistic, shy, and fatigued, and they have a tendency to respond intensely to signals of aversive stimuli, whereas those scoring low on HA characterize themselves as optimistic and outgoing risk-takers [12]. Amygdala 'hyperactivity' in affective disorders is commonly found and in depressed patients increases in amygdala activity have been reported to be lateralized to the left [14] (however, see [37]).Paying special attention to the salient nature of the mood inducing stimuli, in this study we reinvestigate the hypothesis that individual scores on the temperament dimension HA might influence the observation of predominantly left-sided amygdala processing. We have used an adapted paradigm with baby faces as visual 'emotional' stimuli to induce approach and withdrawal-related emotions. We 4 inc...