1997
DOI: 10.1023/a:1007308616686
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Abstract: In our experience the test instrument was simple and easy to operate. It met the specification for precision and accuracy, its measurements were highly linear, and it maintained a stable calibration for one year. If the cuvettes are filled as soon as blood is drawn, anticoagulation is unnecessary. However, the cuvettes should be read with 1 min of filling the cuvette.

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Cited by 13 publications
(3 citation statements)
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“…The reference method for assessing SvO 2 requires mixed venous blood sampling through a pulmonary artery catheter and direct measurement of hemoglobin saturation using a multi-wavelength spectrophotometer (co-oximeter) [ 32 ]. When the blood sampling procedure is correct and the sample is immediately analyzed using a properly calibrated co-oximeter, the SvO 2 measurement is accurate (bias <0.5%), precise (2 standard deviations (SD)/mean =1.3%) and linear (R 2 = 1) [ 33 , 34 ]. Even with such good performance indices, however, the least significant change in a unique measurement (2√2 × 2SD/mean) is 3.7%, meaning that a SvO 2 value of 65% needs to change to >68.7% or <61.3% to have a 95% chance of being real.…”
Section: Metrological Considerationsmentioning
confidence: 99%
“…The reference method for assessing SvO 2 requires mixed venous blood sampling through a pulmonary artery catheter and direct measurement of hemoglobin saturation using a multi-wavelength spectrophotometer (co-oximeter) [ 32 ]. When the blood sampling procedure is correct and the sample is immediately analyzed using a properly calibrated co-oximeter, the SvO 2 measurement is accurate (bias <0.5%), precise (2 standard deviations (SD)/mean =1.3%) and linear (R 2 = 1) [ 33 , 34 ]. Even with such good performance indices, however, the least significant change in a unique measurement (2√2 × 2SD/mean) is 3.7%, meaning that a SvO 2 value of 65% needs to change to >68.7% or <61.3% to have a 95% chance of being real.…”
Section: Metrological Considerationsmentioning
confidence: 99%
“…However, these stains suffer from a number of drawbacks, including significant incubation times, the requirements for washing steps, the need for fixation (which disrupts morphology) and variability in stain In addition to microscopy, the Sight OLO also measures hemoglobin using an unlysed, reagent-free process utilizing four wavelengths to account for absorption and scattering. 13 The hemoglobin chamber is directly filled with undiluted whole blood using capillary action and contains several measurement areas, which differ by optical path length. These areas are used to derive differential measurements in order to normalize for system parameters such as illumination intensity and manufacturing tolerances.…”
Section: Staining and Multispectral Imagingmentioning
confidence: 99%
“…Automated differential spectrophotometry was later developed, which uses double-laser beams to determine the difference in absorbance of a sample compared to a negative sample, thus with this method, matrix effects are accounted for, resulting in better accuracy. CO-oximetry is a measurement technique based on multiple wavelength spectrophotometry, which uses the multiple wavelengths up to the full range of wavelengths for analysis, allowing for more accurate measurement of COHb [20][21][22]. They are currently the standard analytical technique used for measurement of COHb, either with a separate instrument or, for hospital cases, integrated into a BGA [18,23,24].…”
Section: Analytical Techniquesmentioning
confidence: 99%