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Cruz, J.M.; Domínguez, José Manuel; Domı́nguez, Herminia; Parajó, Juan Carlos

doi:10.1023/a:1005693709338

Cited by 39 publications

(5 citation statements)

References 14 publications

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“…In addition, the virulence of several human pathogens has been related to certain cell forms. Indicative of this interest in the dimorphic growth of biotechnologically and/or medically important micro-organisms are reviews that have appeared in the international literature on this subject (San-Blas et al, 1984;O'Shea & Walsh, 1996;Cruz et al, 2000) and not restricted to specific taxonomic groups, as dimorphism is found in several representatives of Basidiomycetes, Ascomycetes, Zygomycetes and imperfect fungi (GuevaraOlvera et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Although no other observations have been reported for the effect of DOC on Y. lipolytica morphology, some reports have correlated oxygen with dimorphism in other dimorphic yeasts. In continuous culture of Debaryomyces hansenii and Kluyveromyces marxianus grown at low dilution rates and restricted aeration, the yeast-to-mycelia transition was correlated with low DOC (Cruz et al, 2000;O'Shea & Walsh, 2000). By contrast, Walker & O'Neill (1990) indicated that ; pH 6±0.5; temperature, 28±1 6C; agitation, 180 r.p.m.…”

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confidence: 96%

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Morphological and metabolic shifts of Yarrowia lipolytica induced by alteration of the dissolved oxygen concentration in the growth environment

et al. 2014

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Yarrowia lipolytica, an ascomycete with biotechnological potential, is able to form either yeast cells or hyphae and pseudohyphae in response to environmental conditions. This study shows that the morphology of Y. lipolytica, cultivated in batch cultures on hydrophilic (glucose and glycerol) and hydrophobic (olive oil) media, was not affected by the nature of the carbon source, nor by the nature or the concentration of the nitrogen source. By contrast, dissolved oxygen concentration (DOC) should be considered as the major factor affecting yeast morphology. Specifically, when growth occurred at low or zero DOC the mycelial and/or pseudomycelial forms predominated over the yeast form independently of the carbon and nitrogen sources used. Experimental data obtained from a continuous culture of Y. lipolytica on glycerol, being used as carbon and energy source, demonstrated that the mycelium-to-yeast form transition occurs when DOC increases from 0.1 to 1.5 mg l "1 . DOC also affected the yeast physiology, as the activity of enzymes implicated in lipid biosynthesis (i.e. ATP-citrate lyase, malic enzyme) was upregulated at high DOC whereas the activity of enzymes implicated in glycerol assimilation (such as glycerol dehydrogenase and kinase) remained fundamentally unaffected in the cell-free extract.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

Morphological and metabolic shifts of Yarrowia lipolytica induced by alteration of the dissolved oxygen concentration in the growth environment

et al. 2014

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“…1 As an alternative method, microbial production of xylitol is becoming more attractive, since the downstream processing is expected to be cheaper and, unlike the chemical route, mild reaction conditions are required. 16 Many studies have been conducted to produce xylitol from the hemicellulose portion of agro-residues, like rice straw, 18 corn cob, 19 brewer's spent grain, 20 sugarcane bagasse, 21 corn stover, 22 barley bran 23 and corn fibre 24 using microbial processes. Xylitol can be produced by some bacteria and filamentous fungi, but the best producers are yeasts, especially species of genus Candida, such as C. guilliermondii, C. pelliculosa, C. parapsilosis, C. tropicalis and C. boidinii.…”

Section: Introductionmentioning

confidence: 99%

Integrated Process of Arabinose Biopurification and Xylitol Fermentation Based on the Diverse Action of Candida boidinii

Fehér

Gazsó

Gál

et al. 2016

Chem. Biochem. Eng. Q.

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Hemicellulosic hydrolysates of agro-residues are promising raw materials for xylitol and arabinose production through biotechnological methods. Two-step acidic fractionation of corn fibre was developed to produce a glucose-and arabinose-rich hydrolysate and a xylose-rich hydrolysate. An integrated process of arabinose biopurification on the glucose-and arabinose-rich hydrolysate and xylitol fermentation on the xylose-rich hydrolysate using Candida boidinii NCAIM Y.01308 was introduced, in which cell mass produced in arabinose biopurification was used as inoculum in the xylitol fermentation. Aerobic biopurification resulted in an arabinose solution containing 9.2 g L-1 of arabinose with a purity of 90 %, based on total sugars. Xylitol fermentation under microaerobic conditions resulted in a xylitol yield of 53 % of theoretical and a xylitol concentration of 10.4 g L-1 in three days. Hence, an integrated biorefinery process of hemicellulosic hydrolysates was developed based on the diverse action of C. boidinii to purify arabinose and produce xylitol.

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“…Physicochemical refining ( − ) and/or biological detoxification ( − ) may be required to improve the bioconversion. The biotechnological production of xylitol from a variety of xylan-containing feedstocks (including corncobs) has been studied in recent years ( − ).…”

Section: Introductionmentioning

confidence: 99%

Purification of Xylitol Obtained by Fermentation of Corncob Hydrolysates

Rivas

Torre

Domínguez

et al. 2006

J. Agric. Food Chem.

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Hydrolysates obtained by autohydrolysis-posthydrolysis of corncobs were detoxified with charcoal, concentrated, supplemented with nutrients, and fermented with Debaryomyces hansenii. After biomass removal, the fermented media contained 0.1137 kg of nonvolatile components (NVC)/kg of liquor, which corresponded mainly to xylitol (0.6249 kg/kg of NVC) but also to minor amounts of inorganic components (measured as ashes), proteins, nonfermented sugars (xylose and arabinose), uronic acids, arabitol, and other nonvolatile components (ONVC). The media were subjected to further processing (sequential stages of adsorption, concentration, ethanol precipitation, concentration, and crystallization) to obtain food-grade xylitol. Adsorption experiments were carried out at various solid-to-liquor ratios. Under selected conditions (1 kg of charcoal/15 kg of liquors), the xylitol content increased to 0.6873 kg/kg of NVC, and almost total decoloration was achieved. The resulting liquor was concentrated by evaporation to increase its NVC content to 0.4032 kg/kg of liquor (corresponding to a xylitol concentration of 0.280 kg/kg of liquor), and ethanol was added to precipitate a part of the NVC (mainly proteins, but also uronic acids, ashes, and other nonvolatile compounds). Refined liquors (containing 0.7303 kg of xylitol/kg of NVC) were concentrated again, and ethanol was added (to reach 40-60% volume of the stream) to allow crystallization at -10 or -5 degrees C. Under selected conditions, 43.7% of xylitol contained in the initial fermentation broth was recovered in well-formed, homogeneous crystals, in which xylitol accounted for 98.9% of the total oven-dry weight. Material balances are presented for the whole processing scheme considered in this work.

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Cited by 39 publications

References 14 publications

Morphological and metabolic shifts of Yarrowia lipolytica induced by alteration of the dissolved oxygen concentration in the growth environment

Morphological and metabolic shifts of Yarrowia lipolytica induced by alteration of the dissolved oxygen concentration in the growth environment

Integrated Process of Arabinose Biopurification and Xylitol Fermentation Based on the Diverse Action of Candida boidinii

Purification of Xylitol Obtained by Fermentation of Corncob Hydrolysates

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