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Abstract

Curcumin is used as a potent anti-inflammatory agent in the treatment of rheumatoid arthritis and its clinical pharmacokinetics requires a sensitive method for estimation of its plasma concentration. Hence the aim of the study was to develop a rapid, selective, and sensitive HPLC method coupled with UV detection for the determination of Curcumin in rat plasma. The process of elution was carried out on Phenomenex Luna C18 (250*4.6 mm id, 5 μm particle size, 130 Å) column using acetonitrile and 1 % formic acid mixture in the ratio of 70:30 % v/v as mobile phase delivered with a flow rate of 1.0 mL/min. Detection was carried out using a UV detector at 420 nm. The method was found to be linear in the range of 100 to 3000 ng/ml with R2 close to one (0.9969). The limit of detection (LOD) and limit of quantification (LOQ) of CUR was found to be 2.82 ng/mL and 8.5 ng/mL, respectively. The method was validated for accuracy, precision, linearity, LOD, LOQ, and robustness. Hence the method developed can be used in routine analysis of curcumin in rat plasma which can be useful to determine the pharmacokinetics of curcumin.