Untitled

Abstract

Present study was aimed to evaluate the preliminary pharmacognosy properties such as antimicrobial, antioxidant and anti-cancer potential of ethanol extract of Cissus quadrangularis. Antimicrobial activity was tested against Staphylococcus aureus, Bacillus subtilis, Vibrio cholera, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Entero toxigenic Excherichia coli (ETEC) andSalmonella typhi by disc diffusion assay. Antioxidant activity was determined by DPPH free radical scavenging assay and ABTS free radical scavenging assay. MCF-7 breast cancer cells were used to investigate the in vitro anti-cancer activity of ethanol extracts of cissus. The anticancer activity MTT assay carried out using MCF7 cell line. The results CQE extract showed more activity against Vibrio parahaemolyticus, Vibrio cholera, ETEC and Salmonella typhi at 5-25 mg/well. The extract also exhibited dose dependent scavenging of DPPH and ABTS radicals. DPPH radicals was more efficient than that of ABTS radicals as revealed by the low IC50 value. MCF-7 breast cancer cells were used to investigate the in vitro anti-cancer activity of ethanol extracts of Cissus. The MTT assay results showed considerable anti-cancer activity in MCF-7 cancer cells, with an IC50 value of 40 µg/ml. Cissus extract effectively inhibited proliferation of MCF-7 cells in a dose-dependent manner over 24h. Thus the compounds of Cissus quadrangularis displayed potential for antioxidant, antibacterial and anti-cancer agents.