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Abstract

MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles by repressing translation or cleaving RNA transcripts. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the potential involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes.In our study, we examined by Real-Time PCR the expression of 156 mature miRNA in colorectal cancer. The analysis by several bioinformatics algorithms of colorectal tumours and adjacent nonneoplastic tissues from patients and colorectal cancer cell lines allowed identifying a group of 13 miRNA whose expression is significantly altered in this tumor. The most significantly deregulated miRNA being miR-31, miR-96, miR-133b, miR-135b, miR-145, and miR-183. In addition, the expression level of miR-31 was correlated with the stage of CRC tumor.Our results suggest that miRNA expression profile could have relevance to the biological and clinical behavior of colorectal neoplasia.

Profiling MicroRNA Expression in Hepatocellular Carcinoma Reveals MicroRNA-224 Up-regulation and Apoptosis Inhibitor-5 as a MicroRNA-224-specific Target

“…Expression of 157 verified human microRNAs was analyzed using the TaqMan MicroRNA assay human panel early access kit (Applied Biosystems), according to the manufacturer's instructions as previously described (27). Briefly, patient RNA samples were used as template for reverse transcription.…”

A computational screen for mouse signaling pathways targeted by microRNA clusters

“…Second, in colorectal cancer cell lines, all three members of this cluster show consistent upregulations. Forkhead family of transcription factors including CHES 1, FOXF2, FOXK2, FOXO1A, FOXO3A, and FOXQ1, were proposed as putative targets (Bandres et al 2006). Finally, Xu et al (2007) reported that Mitf, a transcription factor required for the establishment and maintenance of retinal pigmented epithelium, is a direct target of miR-96 and miR-182, indicating that this miRNA cluster is also important for retinal development and function.…”

Real-time PCR quantification of precursor and mature microRNA

“…A disadvantage of real-time PCR profiling of gene expression include difficulties in transferring small volumes of liquid into 384-well plates. Profiling of both precursor [17][18][19] and mature miRNA [30][31][32][33] have been successfully used in this format. In fact, real-time PCR was used to profile 220 human miRNAs from a single embryonic stem cell [34].…”