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Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

“…Another case where co‐immobilization of enzymes is a requisite is in the design of Redox reactions where not only recycling of the cofactor is intended, but also its reuse, with two immobilized enzymes and an immobilized cofactor 37. There is an example of the use of CLECs of one redox enzyme prepared with the cofactor presented during crystallization, and using two substrates of the enzyme to regenerate the cofactor 38. Examples may be found using porous pre‐existing solids and both (the target enzyme and the cofactor regenerating one) Red‐ox enzymes co‐immobilized in the same particle 39.…”

Biomimetic NAD+ Models for Tandem Cofactor Regeneration, Horse Liver Alcohol Dehydrogenase Recognition of 1,4-NADH Derivatives, and Chiral Synthesis

“…Although the economic benefits of this discovery can not be fully evaluated at this time for biocatalytic applications, it is clear from the presented results, and previous observations,15–17 that some structural features of 1,4‐NADH need not be present for enzyme recognition and for the synthesis of chiral organic compounds. We hope that our results pave the way for the possible utilization of NAD + models such as 1 and 4 in a variety of biocatalytic processes of industrial importance; model 1 and other potential analogues are more stable under conditions that might cause the NAD + to be hydrolytically compromised 26, 27…”

Protein Crystals as Novel Catalytic Materials

“…This idea was first introduced by Lee et al,68 who demonstrated good activity (26 % of that in solution) for crystals of horse liver alcohol dehydrogenase (HLADH) and increased stability of the cross‐linked crystals in the presence of zinc salts. By using this system we demonstrated that the HLADH‐NAD(H)‐CLEC system exhibited high activity (64 % of that in solution) without addition of exogenous NADH 118. This complex was applied to the reduction of additional ketones 20 – 24 (Figure 8).…”