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Anderle, Pascale; Rakhmanova, Vera A.; Woodford, Katie; Zerangue, Noa; Sadée, Wolfgang

doi:10.1023/a:1022282221530

Cited by 48 publications

(23 citation statements)

References 35 publications

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“…4,8 The Affymetrix GeneChip oligonucleotide array data reported earlier 4 was obtained using the Affymetrix GeneChip U95A and was purchased from Affymetrix Inc. The preparation of the custom-designed spotted oligonucleotide array used to obtain expression data for transporter and ion channel genes has been described previously.…”

Section: Methodsmentioning

confidence: 99%

“…The preparation of the custom-designed spotted oligonucleotide array used to obtain expression data for transporter and ion channel genes has been described previously. 8 Preparation of mRNA, Labeling, Hybridization, and Scanning…”

Section: Methodsmentioning

confidence: 99%

“…7 Oligonucleotide arrays can also be prepared by spotting presynthesized oligonucleotide probes (50 to 70 mers) onto chemically treated glass slides. 8,9 Microarrays can also be obtained by spotting cDNA probes onto a slide. 10 The latter provides for good sensitivity but may suffer from lower specificity if closely related genes are measured, owing to potential cross-hybridizations.…”

Section: Introductionmentioning

confidence: 99%

“…4,8 Thus, Sun et al 4 reported Caco-2 gene expression profiles obtained using the Affymetrix GeneChip microarray, whereas Anderle et al 8 reported changes in transporter and ion channel mRNA expression levels on Caco-2 cell differentiation obtained using a custom-designed, spotted 70-oligomer oligonucleotide microarray. 8 The genes examined are involved in the intestinal transport of a variety of therapeutically important entities. 8,20 The major objective of this report was to compare mRNA expression in Caco-2 cells for genes encoding transporters and ion channels measured in the 2 studies.…”

Section: Introductionmentioning

confidence: 99%

“…8 The genes examined are involved in the intestinal transport of a variety of therapeutically important entities. 8,20 The major objective of this report was to compare mRNA expression in Caco-2 cells for genes encoding transporters and ion channels measured in the 2 studies. A second objective was to evaluate measured differences in expression changes upon differentiation of Caco-2 cells obtained using different clones and slightly different culturing conditions.…”

Section: Introductionmentioning

confidence: 99%

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Transporter and ion channel gene expression after caco-2 cell differentiation using 2 different microarray technologies

Landowski

Anderle

Sun

et al. 2004

AAPS J

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ABSTRACTmRNA expression profiles had previously been measured in Caco-2 cells (human colonic carcinoma cells) using either custom-designed spotted oligonucleotide arrays or Affymetrix GeneChip oligonucleotide arrays. The Caco-2 cells used were from different clones and were examined under slightly different culture conditions commonly encountered when Caco-2 cells are used as a model tissue for studying intestinal transport and metabolism in different laboratories. In this study, we compared gene expression profiles of Caco-2 cells generated with different arrays to assess the validity of conclusions derived from the 2 independent studies, with a focus on changes in transporter and ion channel mRNA expression levels on Caco-2 cell differentiation. Significant changes in expression levels upon differentiation were observed with 78 genes, with probes common to both arrays. Of these, 18 genes were upregulated and 36 genes were downregulated. The 2 arrays yielded discrepant results for 24 genes, showing significant changes upon differentiation. The results from the 2 arrays correlated well for genes expressed above average levels (r = 0.75, P < 0.01, n = 25) and poorly for genes expressed at low levels (r = 0.08, P > 0.05, n = 25). Overall correlation across the 2 platforms was r = 0.45 (P < 0.01) for the 78 genes, with similar results from both arrays. Despite differences in experimental conditions and array technology, similar results were obtained for most genes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transporter and ion channel gene expression after caco-2 cell differentiation using 2 different microarray technologies

Landowski

Anderle

Sun

et al. 2004

AAPS J

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Transporters in the Gastrointestinal Tract

Anderle

Nielsen

2008

Methods and Principles in Medicinal Chemistry

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Oxalate Flux Across the Intestine: Contributions from Membrane Transporters

Whittamore

Hatch

2021

Comprehensive Physiology

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Epithelial oxalate transport is fundamental to the role occupied by the gastrointestinal (GI) tract in oxalate homeostasis. The absorption of dietary oxalate, together with its secretion into the intestine, and degradation by the gut microbiota, can all influence the excretion of this nonfunctional terminal metabolite in the urine. Knowledge of the transport mechanisms is relevant to understanding the pathophysiology of hyperoxaluria, a risk factor in kidney stone formation, for which the intestine also offers a potential means of treatment. The following discussion presents an expansive review of intestinal oxalate transport. We begin with an overview of the fate of oxalate, focusing on the sources, rates, and locations of absorption and secretion along the GI tract. We then consider the mechanisms and pathways of transport across the epithelial barrier, discussing the transcellular, and paracellular components. There is an emphasis on the membrane-bound anion transporters, in particular, those belonging to the large multifunctional Slc26 gene family, many of which are expressed throughout the GI tract, and we summarize what is currently known about their participation in oxalate transport. In the final section, we examine the physiological stimuli proposed to be involved in regulating some of these pathways, encompassing intestinal adaptations in response to chronic kidney disease, metabolic acid-base disorders, obesity, and following gastric bypass surgery. There is also an update on research into the probiotic, Oxalobacter formigenes, and the basis of its unique interaction with the gut epithelium.

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Abstract: Although Caco-2 cells have proven a suitable model for studying carrier-mediated transport in human intestines, the expression of specific transporter and ion channel genes may differ substantially.

Cited by 48 publications

References 35 publications

Transporter and ion channel gene expression after caco-2 cell differentiation using 2 different microarray technologies

Transporter and ion channel gene expression after caco-2 cell differentiation using 2 different microarray technologies

Transporters in the Gastrointestinal Tract

Oxalate Flux Across the Intestine: Contributions from Membrane Transporters

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