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Ayisi, Christian Larbi; Zhao, Jintong

doi:10.4194/1303-2712-v17_2_20

Cited by 25 publications

(17 citation statements)

References 31 publications

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“…The effects of replacing FO with PO on liver lipid and FA composition have been comprehensively reported and discussed in a study directly related to this study (Ayisi & Zhao, 2017). Highlights are shown in…”

Section: Liver Lipid and Fatty Acid Compositionmentioning

confidence: 97%

“…T stands for total number of culture days. Mineral mix and vitamin premix were described in (Ayisi & Zhao, 2017). c Nitrogen-free extract, calculated as 1,000 − (protein + lipid + ash) g/kg.…”

Section: Growth and Feed Evaluationmentioning

confidence: 99%

“…In our previous study, the mRNA expression of genes involved in FA synthesis such as fatty acid synthases (FAS), acetyl-CoA carboxylase, stearoyl-CoA desaturase 1 and ATP citrate lyase was significantly increased as PO levels increased (Ayisi & Zhao, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Replacing fish oil with palm oil: Effects onmRNAexpression of fatty acid transport genes and signalling factors related to lipid metabolism in Nile tilapia (Oreochromis niloticus)

et al. 2018

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This study evaluated the effects of replacing fish oil (FO) with palm oil (PO) on enzyme activities and mRNA expression of lipid transport genes and signalling factors in juvenile Nile tilapia. Nile tilapia (9.34 ± 0.14 g) was fed five experimental diets with FO supplemented with PO at 0%, 25%, 50%, 75% and 100%. Liver was sampled from five fish per tank for analysis of fatty acids, enzyme activities and mRNA expression. Glucose 6‐phosphate dehydrogenase (G6PD) was highly expressed in groups fed higher levels of dietary PO, whilst liver X receptor (LXR) was highly expressed in groups fed lower levels of dietary PO. Groups fed 50% PO, 75% PO and 100% PO had significantly higher levels of G6PD mRNA expression whilst group fed 100% PO had significantly higher level of lipoprotein lipase (LPL) mRNA expression. Groups fed 75% PO and 100% PO recorded higher levels of PPAR‐α mRNA expression whilst higher level of malic enzyme mRNA expression was observed in group fed 75% PO. The mRNA expressions of the above stated genes and enzymes activities are predominantly influenced by dietary n‐3 PUFA and n‐6 PUFA levels. Dietary PO inclusion affected enzyme activities of LXR, PPAR‐α,PPAR‐β, LPL and FABP‐3. Activities of PPAR‐α, PPAR‐β and FABP‐3 were increased by PO inclusion whilst LXR and ELOVL 5 were reduced by PO inclusion. In conclusion, it could be inferred that substituting FO with PO could alter the metabolism pathway of genes such as LXR, G6PD, PPAR‐α, LPL and ME that are involved in the transport of fatty acids.

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Section: Liver Lipid and Fatty Acid Compositionmentioning

confidence: 97%

Section: Growth and Feed Evaluationmentioning

confidence: 99%

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Replacing fish oil with palm oil: Effects onmRNAexpression of fatty acid transport genes and signalling factors related to lipid metabolism in Nile tilapia (Oreochromis niloticus)

et al. 2018

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“…Six isonitrogenous, isolipidic and isoenergetic experimental diets were formulated as shown in Table 1 and thoroughly mixed using the progressive enlargement method as previously described by Ayisi and Zhao (2017). The mixture of dry ingredients was then mixed with virgin coconut oil, corn oil and fish oil with distilled water in a Hobart mixer, and the resulting moist dough pelleted through a pellet extruder of 1-mm die.…”

Section: Feed and Feeding Trialmentioning

confidence: 99%

“…Real-time PCR machine (Bio-Rad). The reaction was performed following protocols as previously described in Ayisi and Zhao (2017) and Livak and Schmittgen (2001…”

Section: Rna Extraction and Real-time Quantitative Polymerase Chainmentioning

confidence: 99%

Effects of alternative dietary oils on immune response, expression of immune‐related genes and disease resistance in juvenile Nile tilapia,Oreochromis niloticus

2019

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The expression of immune‐related genes and immune responses to Aeromonas hydrophila were investigated on Oreochromis niloticus (6.07 ± 0.07 g), by feeding them six different diets for 8 weeks to apparent satiation. Diets contained fish oil (60g/kg FO), virgin coconut oil (60g/kg VCO) and corn oil (60g/kg CO) as sole lipids or blends of 30g/kg FO + 30g/kg VCO (3FVCO), 30g/kg FO + 30g/kg CO (3FCO) and 30g/kg VCO + 30g/kg CO (VO). Fish fed 3FCO recorded higher final weight, percentage weight gain (%WG) and specific growth rate (%SGR) but not significantly higher than all other groups. Triglyceride was higher in fish fed 3FCO than 3FVCO and CO (p ˂ 0.05), whereas total immunoglobulin (TIg) was not significant (p ˂ 0.05) between groups. Lysozyme activity was significantly higher in fish fed diet CO while groups FO, 3FCO and VCO recorded the least activities (p ˂ 0.05). Although alternative complement activity (ACH50), complement proteins (C3 and C4), was not influenced, antibody titre production was significantly higher in fish fed diet 3FCO and lower in group CO. mRNA expression of IL‐1β was significantly upregulated in fish fed VO while the expression of C‐type lysozyme and TGF‐β was not significantly influenced across treatments, although group fed FO recorded higher expression levels, respectively. Lower mortalities of fish were recorded in groups fed 3FCO and VO after 14 days postchallenge with A. hydrophila disease indicating the enhancing effects of vegetable oils to boost immune response and resistance to disease. The study concludes that alternative lipid sources with high polyunsaturated fatty acids (PUFAs‐ALA and LA) (CO) and blend of saturated fatty acids (SFA)(VCO) can partially and or exclusively replace FO with an improved effect on tilapia and resistance to A. hydrophila in tilapia.

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