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Liu, Yu; Xu, Hai-Lou; Show, Kuan‐Yeow; Tay, Joo‐Hwa

doi:10.1023/a:1014459006210

Cited by 114 publications

(20 citation statements)

References 80 publications

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“…The research study conducted by Liu et.al. in 2002 [46], proposed that abundant Methanosaeta spp. improves granulation and consequently leads to more stable reactor performance.…”

Section: Methodsmentioning

confidence: 99%

Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

et al. 2018

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Introduction:Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence in situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique.Methods & Materials:Fluorescence in situ hybridization (FISH) was performed to detect and quantify the methanogenic Archaea in the sludge samples whereas qPCR was carried out to support the FISH analysis. Multiple probes targeting domain archaea, different orders and families of Archaea were used for the studies.Results and Discussion:In general, the aceticlastic organisms (Methanosarcinaceae & Methanosaetaceae) were more abundant than the hydrogenotrophic organisms (Methanobacteriales, Methanomicrobiales, Methanobacteriaceae & Methanococcales). Both FISH and qPCR indicated that family Methanosaetaceae was the most abundant suggesting that aceticlastic methanogenesis is probably the dominant methane production pathway in these digesters.Conclusion:Future work involving high-throughput sequencing methods and correlating archaeal communities with the main operational parameters of anaerobic digesters will help to obtain a better understanding of the dynamics of the methanogenic archaeal community in wastewater treatment plants in United Arab Emirates (UAE) which in turn would lead to improved performance of anaerobic sludge digesters.

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“…The research study conducted by Liu et.al. in 2002 [46], proposed that abundant Methanosaeta spp. improves granulation and consequently leads to more stable reactor performance.…”

Section: Methodsmentioning

confidence: 99%

Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

et al. 2018

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“…Anaerobic granules are small, with diameters generally ranging 0.1-5.0 mm (Ahn, 2000;Batstone and Keller, 2001;Shin et al, 2019), densely-packed biofilm spheres. They comprise a complex microbial community capable of the complete mineralization of organic pollutants through the anaerobic digestion (AD) pathway (Liu et al, 2002;Batstone et al, 2019). The settleabilty of anaerobic granules accounts for long biomass retention-even in "upflow" bioreactors, such as the upflow anaerobic sludge bed (UASB) and expanded granular sludge bed (EGSB) bioreactors, operated with short hydraulic retention times (HRT), and very high volumetric loading and upflow velocities (van Lier et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Growth and Break-Up of Methanogenic Granules Suggests Mechanisms for Biofilm and Community Development

et al. 2020

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Methanogenic sludge granules are densely packed, small, spherical biofilms found in anaerobic digesters used to treat industrial wastewaters, where they underpin efficient organic waste conversion and biogas production. Each granule theoretically houses representative microorganisms from all of the trophic groups implicated in the successive and interdependent reactions of the anaerobic digestion (AD) process. Information on exactly how methanogenic granules develop, and their eventual fate will be important for precision management of environmental biotechnologies. Granules from a full-scale bioreactor were size-separated into small (0.6-1 mm), medium (1-1.4 mm), and large (1.4-1.8 mm) size fractions. Twelve laboratory-scale bioreactors were operated using either small, medium, or large granules, or unfractionated sludge. After >50 days of operation, the granule size distribution in each of the small, medium, and large bioreactor sets had diversified beyond-to both bigger and smaller than-the size fraction used for inoculation. Interestingly, extra-small (XS; <0.6 mm) granules were observed, and retained in all of the bioreactors, suggesting the continuous nature of granulation, and/or the breakage of larger granules into XS bits. Moreover, evidence suggested that even granules with small diameters could break. "New" granules from each emerging size were analyzed by studying community structure based on high-throughput 16S rRNA gene sequencing. Methanobacterium, Aminobacterium, Propionibacteriaceae, and Desulfovibrio represented the majority of the community in new granules. H2-using, and not acetoclastic, methanogens appeared more important, and were associated with abundant syntrophic bacteria. Multivariate integration (MINT) analyses identified distinct discriminant taxa responsible for shaping the microbial communities in different-sized granules.

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“…Granulation is the result of the self-immobilization of anaerobic microorganisms under certain physicochemical and biological conditions. Although granule formation has been widely studied, this phenomenon occurs within a limited range of wastewater characteristics and reactor designs (Uyanik et al, 2002) since the microstructure of the granules may be affected by the complexity of the substrate (Liu et al, 2002). The extracellular polymeric substances (EPS) play an important role in granulation because they can bind cells closely through ion binding interactions, hydrophobic interactions, and polymer entanglement (Sheng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Granulation and microbial community dynamics in the chitosan-supplemented anaerobic treatment of wastewater polluted with organic solvents

et al. 2018

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The effect of chitosan on the development of granular sludge in upflow anaerobic sludge blanket reactors (UASB) when treating wastewater polluted with the organic solvents ethanol, ethyl acetate, and 1-ethoxy-2-propanol was evaluated. Three UASB reactors were operated for 219 days at ambient temperature with an organic loading rate (OLR) of between 0.3 kg COD m d and 20 kg COD m d. One reactor was operated without the addition of chitosan, while the other two were operated with the addition of chitosan doses of 2.4 mg gVSS two times. The three reactors were all able to treat the OLR tested with COD removal efficiencies greater than 90%. However, the time required to reach stable operation was considerably reduced in the chitosan-assisted reactors. The development of granules in the reactors with chitosan was accelerated and granules larger than 2000 μm were only observed in these reactors. In addition, these granules exhibited better physicochemical characteristics: the mean particle diameter (540 and 613 μm) was approximately two times greater than in the control reactor (300 μm), and the settling velocities exceeded 35 m h. The extracellular polymeric substances (EPS) in the reactors with the chitosan was found to be higher than in the control reactor. The protein-EPS content has been correlated with the granule size. The analyses of the microbial communities, performed through denaturing gradient gel electrophoresis and high-throughput sequencing, revealed that the syntrophic microorganisms belonging to genus Geobacter and the hydrogenotrophic methanogen Methanocorpusculum labreanum were predominant in the granules. Other methanogens like Methanosaeta species were found earlier in the chitosan-assisted reactors than in the control reactor.

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Cited by 114 publications

References 80 publications

Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

Growth and Break-Up of Methanogenic Granules Suggests Mechanisms for Biofilm and Community Development

Granulation and microbial community dynamics in the chitosan-supplemented anaerobic treatment of wastewater polluted with organic solvents

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