Ornamental industry has applied immensely in vitro propagation approach for large-scale plant multiplication at very high rates of elite superior varieties. As a result, hundreds of plant tissue culture laboratories have come up worldwide. Dieffenbachia species are popular foliage potted plants used in interiorescapes of homes, offices, and malls throughout the world. Most of Dieffenbachia species are now propagated by tissue culture for better utilize of species and expedite plant improvement. This review paper summarizes valuable literature on in vitro techniques including type of explants used, media optimized, ways of propagation and improvement through 45 years of research on Dieffenbachia spp. Which were provide basis for future studies such as genetic transformation for breeding aims, develop new cultivars, develop disease-resistant plants and overcome the environmental obstacles. There is a need for more application of the plant tissue culture techniques on Dieffenbachia to investigate the responses of different cultivars and explants to variable culture media.