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Jiménez, Elio; Pérez, Naivy; Feria, Manuel de; Barbón, Raúl; Capote, Alina; Chávez, Maité; Quiala, Elisa; Pérez, Juan Carlos

doi:10.1023/a:1006312029055

Cited by 58 publications

(13 citation statements)

References 14 publications

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“…In the previous studies, microtubers were mostly formed at the surface of the medium, although tuberization was observed in all parts of plants grown in jar fermentors with semi-continuous medium surface level control Takayama 1993, 1994) and in a temporary immersion system (Jiménez et al 1999). In contrast, microtubers were formed from the surface of the medium up to the tops of the culture bags in the present study.…”

Section: Discussioncontrasting

confidence: 59%

Production of potato (<i>Solanum tuberosum</i>, L.) microtubers using plastic culture bags

Mamiya

Tanabe

Onishi

2020

Plant Biotechnology

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We have developed a system using plastic culture bags with forced aeration system for both liquid medium and gaseous phase to produce microtubers of potato (Solanum tuberosum L.). The production of microtubers under sterile conditions is a good way to produce disease-free materials for crop production, and bioreactors have been used for this purpose. However, bioreactors are expensive and difficult to handle. The plastic culture bags are relatively inexpensive and are easy to store and sterilize because they can be flattened. Microtuber production involves two stages: plant proliferation in one medium, followed by microtuber production in a different medium. Both steps are carried out using the same culture bag. Using this system, we produced 100 to 300 microtubers per 8 l culture bag, depending on the potato cultivar. We varied the nutrient concentrations in the media and found that a lower sucrose concentration in the plant proliferation medium and lower nitrogen concentration in the microtuber production medium both increased the total numbers of microtubers per bag. Notably, a higher concentration of potassium phosphate increased the numbers of larger microtubers. This is beneficial because larger microtubers are much more tolerant to field conditions. We produced about 250,000 microtubers per year in a 66 m 2 tissue culture room using the culture bag system. These microtubers have been planted directly in the field and utilized for seed potato production.

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Section: Discussioncontrasting

confidence: 59%

Production of potato (<i>Solanum tuberosum</i>, L.) microtubers using plastic culture bags

Mamiya

Tanabe

Onishi

2020

Plant Biotechnology

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“…With 1 min immersion every 12 or 24 h, the productivity of TIS was comparable to the SmartSett® system. The reduction in shoot numbers for immersion every 6 or 48 h may be due to media being frequently or infrequently delivered to the system, respectively, which may imbalance nutrient and growth regulator uptake (Alvard et al 1993;Jimenez et al 1999;Berthouly and Etiene 2002). With increased immersion, an aqueous cover can form on the plant tissue and reduce gas exchange to the cell surface, which is detrimental to meristem formation (Jackson 2002).…”

Section: Discussionmentioning

confidence: 99%

Development of a temporary immersion system (RITA®) for mass production of sugarcane (Saccharum spp. interspecific hybrids)

Mordocco¹,

Brumbley²,

Lakshmanan³

2008

In Vitro Cell.Dev.Biol.-Plant

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Commercial micropropagation of sugarcane is largely determined by the clonal fidelity and the cost of plants produced. Rapid production of plants in vitro reduces the frequency of offtypes in many species. By exploiting the concept of transverse thin cell layer culture, we have developed a rapid, high frequency direct plant regeneration system, called SmartSett®, for commercial sugarcane cultivars grown in Australia. Similar to conventional micropropagation, labour remains the major cost of this plant production system. Hence, to reduce the labour component, we have integrated the SmartSett® system with the RITA® temporary immersion bioreactor. Thin transverse leaf sections or fragmented leaves cultured on agar-based SmartSett® shoot induction medium were used as the starting material for RITA®. Shoot initiation on semi-solid medium prior to transferring to RITA®, culture immersion frequency, explant size and genotype determined the productivity (number of plants produced per unit culture) of the system. Results obtained with cultivar Q165 indicate that explants cultured for 45 d on SmartSett® shoot induction medium were the most prolific, producing on average 275 shoots per vessel after 45 d of culture in RITA with 1 min immersion every 12 or 24 h. Using the fragmented tissue, 14-d-old explants and 3-mm leaf tissue fragments were the most productive. Experiments with three cultivars (Q117, Q165 and Q205) showed that RITA® culture conditions need to be optimised for each cultivar for maximum plant production.

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“…Most protocols fail to produce micro tubers that are larger in size (>0.5 g) (Vreugdenhil et al, 2007). In vitro microtuberization with liquid media has produced larger microtubers (Leclerc et al, 1994;Jimenez et al, 1999). However, the microtubers with liquid culture tend to be soft and develop open lenticels.…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro Microtuberization and Temporary Immersion Bioreactor System to Evaluate Heat Stress Tolerance in Potatoes (Solanum tuberosum L.)

et al. 2021

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High temperature (heat) stress reduces tuber yield and quality of potatoes. Screening potatoes for heat tolerance is increasingly important, considering the climate change scenario and expansion of potatoes to countries where heat stress is an issue. In vitro screening for tolerance to abiotic stresses offers several advantages, including quick evaluation of numerous genotypes (clones) in reduced space, controlled environmental conditions (temperature and photoperiod), and free from confounding variables inherent to greenhouse and field conditions. In this study, we explored the feasibility of using a temporary immersion bioreactor system for heat tolerance screening of potatoes. We determined the best hormone-free microtuberizing media for this system (MSG with 8% sucrose) to enhance microtuber number and size. Comparisons of microtubers produced at 30°C as heat treatment, with 16°C as normal condition, allowed to identify heat tolerant and susceptible potato clones. The use of bioreactors allowed distinguishing well-formed (non-deformed) from deformed microtubers. Heat stress increased the total biomass of plant tissues in all the clones. However, the effect of heat stress on microtuber number and weight varied among the clones. Incubation at 30°C decreased the weight and number of non-deformed microtubers in all the clones except for Reveille Russet in which the weight of non-deformed microtubers was significantly increased and the count of non-deformed microtubers was not affected. The potato variety Reveille Russet, which was selected under high-temperature field conditions in Texas, had many non-deformed microtubers per explant and the highest microtuber weight among four clones evaluated under heat stress. We described a faster and reliable in vitro microtuberization system for abiotic stress tolerance screening, identified Reveille Russet as a promising heat-tolerant potato variety, and confirmed Russet Burbank and Atlantic as susceptible heat-tolerant checks.

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Cited by 58 publications

References 14 publications

Production of potato (<i>Solanum tuberosum</i>, L.) microtubers using plastic culture bags

Production of potato (<i>Solanum tuberosum</i>, L.) microtubers using plastic culture bags

Development of a temporary immersion system (RITA®) for mass production of sugarcane (Saccharum spp. interspecific hybrids)

Development of an in vitro Microtuberization and Temporary Immersion Bioreactor System to Evaluate Heat Stress Tolerance in Potatoes (Solanum tuberosum L.)

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