Untitled

Rangel, M. del Ara; González‐Polo, Rosa A.; Caro, A.; Amo, Eva del; Palomo, Luis; Hernández, Ernesto Arteaga; Soler, Germán; Fuentes, José

doi:10.1007/s102380200007

Cited by 28 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, T cell immune functions are suppressed. As arginase activity has been found to be elevated in many different cancers including breast, prostate, gastric, colorectal, and hepatocellular carcinoma, it can be used as a new prognostic biomarker in in vivo experiments [ 10 11 ]. In this study, we aimed to determine the association of arginase activity in tumor tissues and sera with the tumor growth inhibition induced by different treatment strategies.…”

Section: Introductionmentioning

confidence: 99%

Antitumor Effect of IP-10 by Using Two Different Approaches: Live Delivery System and Gene Therapy

et al. 2016

View full text Add to dashboard Cite

PurposeImmunotherapy is one of the treatment strategies for breast cancer, the most common cancer in women worldwide. In this approach, the patient's immune system is stimulated to attack microscopic tumors and control metastasis. Here, we used interferon γ-induced protein 10 (IP-10), which induces and strengthens antitumor immunity, as an immunotherapeutic agent. We employed Leishmania tarentolae, a nonpathogenic lizard parasite that lacks the ability to persist in mammalian macrophages, was used as a live delivery system for carrying the immunotherapeutic agent. It has been already shown that arginase activity, and consequently, polyamine production, are associated with tumor progression.MethodsA live delivery system was constructed by stable transfection of pLEXSY plasmid containing the IP-10-enhanced green fluorescent protein (IP-10-egfp) fusion gene into L. tarentolae. Then, the presence of the IP-10-egfp gene and the accurate integration location into the parasite genome were confirmed. The therapeutic efficacy of IP-10 delivered via L. tarentolae and recombinant pcDNA-(IP-10-egfp) plasmid was compared by determining the arginase activity in a mouse 4T1 breast cancer model.ResultsThe pcDNA-(IP-10-egfp) group showed a significant reduction in tumor weight and growth. Histological evaluation also revealed that only this group demonstrated inhibition of metastasis to the lung tissue. The arginase activity in the tissue of the pcDNA-(IP-10-egfp) mice significantly decreased in comparison with that in normal mice. No significant difference was observed in arginase activity in the sera of mice receiving other therapeutic strategies.ConclusionOur data indicates that IP-10 immunotherapy is a promising strategy for breast cancer treatment, as shown in the 4T1-implanted BALB/c mouse model. However, the L. tarentolae-(IP-10-EGFP) live delivery system requires dose modifications to achieve efficacy in the applied regimen (six injections in 3 weeks). Our results indicate that the arginase assay could be a good biomarker to differentiate tumoral tissues from the normal ones.

show abstract

Section: Introductionmentioning

confidence: 99%

Antitumor Effect of IP-10 by Using Two Different Approaches: Live Delivery System and Gene Therapy

et al. 2016

View full text Add to dashboard Cite

show abstract

“… 9 Its expression in breast cancer cells has additionally been reported, 10 and its levels are significantly higher in breast cancer tissue and peripheral blood compared to healthy donors. 11 ARG2 activity is also described as being enhanced in gastric cancer when compared with healthy gastric tissue, 12 and the enzyme is released in vitro by gastric cancer cell lines and in the serum of patients with gastric cancer. 9 , 13 In addition to solid tumors, enhanced ARG2 expression has been described in acute myeloid leukemia (AML).…”

Section: Introductionmentioning

confidence: 99%

The metabolic enzyme arginase-2 is a potential target for novel immune modulatory vaccines

et al. 2020

View full text Add to dashboard Cite

One way that tumors evade immune destruction is through tumor and stromal cell expression of argininedegrading enzyme arginase-2 (ARG2). Here we describe the existence of pro-inflammatory effector T-cells that recognize ARG2 and can directly target tumor and tumor-infiltrating cells. Using a library of 34 peptides covering the entire ARG2 sequence, we examined reactivity toward these peptides in peripheral blood mononuclear cells from cancer patients and healthy individuals. Interferon-γ ELISPOT revealed frequent immune responses against several of the peptides, indicating that ARG2-specific self-reactive T-cells are natural components of the human T-cell repertoire. Based on this, the most immunogenic ARG2 protein region was further characterized. By identifying conditions in the microenvironment that induce ARG2 expression in myeloid cells, we showed that ARG2-specific CD4T-cells isolated and expanded from a peripheral pool from a prostate cancer patient could recognize target cells in an ARG2-dependent manner. In the 'cold' in vivo tumor model Lewis lung carcinoma, we found that activation of ARG2-specific T-cells by vaccination significantly inhibited tumor growth. Immune-modulatory vaccines targeting ARG2 thus are a candidate strategy for cancer immunotherapy.

show abstract

Effect of Occupational Exposure to Aflatoxins on Some Liver Tumor Markers in Textile Workers

Saad-Hussein

Beshir

Moubarz

et al. 2013

American J Industrial Med

View full text Add to dashboard Cite

show abstract

Untitled

Cited by 28 publications

References 20 publications

Antitumor Effect of IP-10 by Using Two Different Approaches: Live Delivery System and Gene Therapy

Antitumor Effect of IP-10 by Using Two Different Approaches: Live Delivery System and Gene Therapy

The metabolic enzyme arginase-2 is a potential target for novel immune modulatory vaccines

Effect of Occupational Exposure to Aflatoxins on Some Liver Tumor Markers in Textile Workers

Contact Info

Product

Resources

About