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Abstract

An epidemiologic study of methicillin-resistant Staphylococcus aureus (MRSA) was conducted by antibiotype, coagulase gene typing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. A total of 129 MRSA strains were isolated from 17 hospitals in the regions of the central, northern, northeastern and eastern Thailand during November 2003 -March 2004. Antimicrobial susceptibility testing with a panel of 10 antimicrobial agents showed 9 different antibiotypes. The antibiotypes 1 and 2 were the most common phenotypes with 44.2% and 35.6% of the isolates, respectively. Coagulase gene typing of MRSA strains generated 4 different genotypes: I, II, III, IV, the PCR products of which were 492±20, 654±20, 735±20 and 816±20 bp with the percentages of 1.5 (2/129), 2.3 (3/129), 82.2 (106/129) and 14 (18/129), respectively. Coagulase gene PCR-RFLPs exhibited 4 patterns: A, B, C and D, with AluI digested PCR product fragments at 220±20 and 220±20 bp (pattern A); 400±20 and 220±20 bp (pattern B); 420±20 and 220±20 bp (pattern C); and 510±20 and 220±20 bp (pattern D). The percentage values for each pattern were compatible with those from the coagulase gene typing method. The results indicated that antibiotypes 1, 2, coagulase gene type III and PCR-RFLP pattern C were the epidemic strains while the rest were sporadic strains.

Genetic Variability of Methicillin Resistant <italic>Staphylococcus Aureus</italic> Strains Isolated from Burns Patients

“…There were 89 MRSA strains isolated from burns patients with 10 coa genes identified by multiplex PCR, similar to the results of Afrogh et al [10], who showed 9 different patterns of coa genes among staff nose, and patients’ clinical samples in Iran. The same results were reported by Janwithayanuchit et al [27] from Thailand, and Omar et al [28] from Egypt. Our study has reported that types III, II, I and VIII were the most common, and accounted for 47.2%, 14.6%, 10.1% and 10.1% of the isolates, respectively.…”

Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018

“…In a research involving 157 S. aureus strains from clinical specimens, nine different patterns of coa gene were detected (Afrough et al, 2013). These findings were confirmed by results in Thailand (Janwithayanuchit et al, 2006) and Egypt (Omar et al, 2014) reported previously. Genetic variability in coa gene among studied isolates indicated that it could not be a predictor for specific iMLSB S. aureus strains.…”

Heterogeneity in &lt;i&gt;femA&lt;/i&gt; in the Indian Isolates of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; Limits Its Usefulness as a Species Specific Marker

“…Variations (polymorphism) in the genomic sequences are not uncommon in S. aureus, even highly conserved and widely used species specific markers like coagulase (coa), Staphylococcal Protein A (spa) genes have shown polymorphisms. Variations in the sequence of the coa and spa genes and the hyper variable region adjacent to the mecA gene, have been the basis for the most widely used forms of PCR typing of MRSA [26][27][28]. Further, it is also known that sequence variations culminate in changes in virulence properties of S. aureus which influence clinical disease manifestations in humans [29].…”