2003
DOI: 10.1023/a:1024116916932
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Abstract: In recent years, several inhibitors that prevent caspase activation and apoptosis have emerged. At high doses, however, these inhibitors can have nonspecific effects and/or become cytotoxic. In this study, we determined the effectiveness of broad spectrum caspase inhibitors to prevent apoptosis. A carboxy terminal phenoxy group conjugated to the amino acids valine and aspartate (Q-VD-OPh) potently inhibited apoptosis. Q-VD-OPh was significantly more effective in preventing apoptosis than the widely used inhibi… Show more

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Cited by 349 publications
(159 citation statements)
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“…In order to test the functional role of caspase-3 in myoblast differentiation, a broad-spectrum caspase inhibitor, Q-VD-OPH (Caserta et al, 2003) was added to the cells prior to induction of differentiation. Addition of 30 μM Q-VD-OPH significantly inhibited caspase-3-like (DEVDase) activity ( Fig.…”
Section: Suppression Of Myoblast Differentiation Using a Broadspectrumentioning
confidence: 99%
“…In order to test the functional role of caspase-3 in myoblast differentiation, a broad-spectrum caspase inhibitor, Q-VD-OPH (Caserta et al, 2003) was added to the cells prior to induction of differentiation. Addition of 30 μM Q-VD-OPH significantly inhibited caspase-3-like (DEVDase) activity ( Fig.…”
Section: Suppression Of Myoblast Differentiation Using a Broadspectrumentioning
confidence: 99%
“…In sharp contrast, neither 7BIO nor Me7BIO induced any activation of caspases, which remained at the level of control, untreated cells. Furthermore, Q-VD-OPh, a general caspase inhibitor (Caserta et al, 2003), had no effect on cell death induced by 7BIO (Figure 10), whereas it reduced the level of cell death induced by 5BIO and 6BIO, and IO, to a lesser extent (Figure 10a). The time course of 7BIO-induced cell death was unaffected by Q-VD-OPh (Figure 10b).…”
Section: -Bromoindirubin-3mentioning
confidence: 99%
“…Such experiments, for example those involving caspase activation and ER stress and that include the use of z-VADfmk or related a-halomethylketone inhibitors should therefore be interpreted carefully. An alternative inhibitor, such as Q-VD-OPH, 11 should be used as a pan-caspase inhibitor to avoid such issues since this compound is without effect on PNGase. 9 Nevertheless, given the contribution that peptide sequence of aspartyl fluoromethylketones make to specificity of caspase inactivation, this same class of inhibitor may serve as a scaffold for designing more potent inhibitors of PNGase and caspases that display greater specificity for their intended targets.…”
mentioning
confidence: 99%