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Nasr‐Esfahani, Mohammad Hossein; Razavi, Shahnaz; Mardani, Mohammad

doi:10.1023/a:1009412130417

Cited by 83 publications

(20 citation statements)

References 39 publications

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“…These results were in agreement with the study of Esterhuizen et al [57] who showed that fertilization rate in ICSI is lower when semen samples with high rates of nuclear condensation are used. Other studies reported that sperm with high rates of blue-stained nucleus can affect fertilization rate following in vitro fertilization procedure and pregnancy rates [58, 59]. Also, the risk of spontaneous abortion would be significantly higher after artificial insemination using ejaculates with high rates of immature nuclear sperm [60, 61].…”

Section: Discussionmentioning

confidence: 99%

“…However, unlike aniline blue staining, they found a significant difference in the percentage of CMA3-positive sperm between the two groups. This difference between AB and CMA3 staining could be due to the fact that CMA3 staining is a more sensitive and specific test and CMA3 reveals not only the presence of excessive histones but also protamine deficiency [59]. Similarly, Razavi et al [49] did not find any correlation between excessive histones evaluated by AB staining and fertilization rate following ICSI.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, there are still controversies over the effect of sperm chromatin status on fertilization rate, embryo quality, and pregnancy outcomes. The use of more sophisticated techniques as chromomycin A3 assay (CAM3) [59] and transmission electron microscopy (TEM) image cytometry [65] provides more accurate and specific results in the evaluation of the sperm nucleus maturity but has many technical constraints to use in routine investigations. We must continue and deepen the study of sperm chromatin quality in infertile men to better understand their relationship with the fertilizing capacity and better assess their impact on the results of in vitro fertilization and embryo development.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

Sellami

Chakroun

Zarrouk

et al. 2013

Advances in Urology

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During spermatogenesis, sperm chromatin undergoes structural changes and results in a high condensation. This nuclear compaction would be useful as a predictor of sperm fertilization capacity and pregnancy outcome. We purpose to evaluate firstly the relationship among chromatin maturity assessed by aniline blue staining (AB) and the semen parameters in infertile men. Secondly, we analyzed whether the sperm gradient density centrifugation is effective to select mature spermatozoa. Fifty-one ejaculates were investigated by semen analysis and stained for chromatin condensation with AB to distinguish between unstained mature sperm and stained immature sperm. AB was applied also on 12 ejaculates which proceeded by density gradient centrifugation to compare the rates of immature sperm before and after selection. Neat semen were divided into two groups: G1 (n = 31): immature sperm <20% and G2 (n = 20): immature sperm ≥20%. No significant differences were detected in sperm concentration, motility, and normal morphology between G1 and G2. However, the rates of some morphology abnormalities were higher in G2: head abnormalities (P = 0.01) and microcephalic sperm (P = 0.02). We founded significant correlation between sperm immaturity and acrosome abnormalities (r = 0.292; P = 0.03). Sperm selection has significantly reduced the rates of immature sperm. A better understanding of chromatin structure and its impact on the sperm potential is needed to explore male infertility.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

Sellami

Chakroun

Zarrouk

et al. 2013

Advances in Urology

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“…Any changes in process of substitution of protamine directly effects on fertilization rate and embryo quality and pregnancy outcome. [36]…”

Section: Discussionmentioning

confidence: 99%

PROTAMINE1 and PROTAMINE2 genes expression in the sperms of oligoasthenospermic individuals and intrauterine insemination candidates couples: Is there any significant differences?

et al. 2016

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Background:Male infertility refers to a male's inability to cause pregnancy in a fertile female. It seems the large portion of this category of infertility, has roots in genetic factors. PROTAMINE family is one of the most important genes which are involved in male factor infertility. Hence, the aim of this study is to evaluate PROTAMINE1 and PROTAMINE2 (P1 and P2) genes expression in oligoasthenospermic individuals and intrauterine insemination (IUI) candidate couples’ sperms.Materials and Methods:Samples were gathered from the patients referred to the Isfahan Infertility Center of Shahid Beheshti, 80 semen samples were in IUI candidates groups and 16 semen samples were in oligoasthenospermia group was collected. The outcome of IUI procedure was followed up after 14 days. Through these samples, 16 couples achieved pregnancy (IUI+) and from the top of the list, 16 semen samples with negative β-HCG were obtained (IUI−). After RNA extraction from sperms, PROTAMINE genes family expression was evaluated in our three groups by real time-reverse transcription polymerase chain reaction.Results:Our study revealed that P1 gene expression has no significant differences between IUI−, IUI+, and oligoasthenospermia groups, whereas P2 gene expression showed significant differences between oligoasthenospermia with two IUI groups. Main sperm parameters have no significant differences between IUI groups.Conclusion:This study reveals P1 and P2 genes expression value have no significant differences between IUI− and IUI+. On the other hand, P2 gene expression value has significant differences between oligoasthenospermia with two IUI groups.

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“…Histone-rich nuclei of immature sperm are rich in lysine and will consequently take up the blue stain. On the other hand, protamine rich nuclei of mature sperm are rich in arginine and cysteine and contain relatively low levels of lysine, which means they will not be stained by Ab (25). A drop of semen was spread on the glass slides and allowed to air-dry.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats

Ebadimanas

2018

Comp Clin Pathol

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Background: Pyridaben, a pyridazinone derivative, is a new acaricide and insecticide for control of mites and some insects such as white flies, aphids and thrips. Objective: This study was designed to elucidate how pyridaben can affect the sperms' morphological parameters, its DNA integrity, and to estimate the effect of various quantities of pyridaben on in vitro fertilization rate. Materials and Methods: In this study, 80 adult male Balb/C strain mice were used. Animals were divided into control and two test groups. Control group received distilled water. The test group was divided into two subgroups, viz, high dose (212 mg/kg/day) and low dose (53 mg/kg/day) and they received the pyridaben, orally for duration of 45 days. The spermatozoa were obtained from caudae epididymides on day 45 in all groups. Sperm viability, protamin compression (nuclear maturity), DNA double-strand breaks, and in vitro fertilizing (IVF) ability were examined. Results: The pyridaben treatment provoked a significant decrease in sperm population and viability in epididymides. The data obtained from this experiment revealed that, the pyridaben brings about negative impact on the sperm maturation and DNA integrity in a time-dependent manner, which consequently caused a significant (p<0.05) reduction in IVF capability. Embryo developing arrest was significantly (p<0.05) higher in treated than the control group. Conclusion: Theses results confirmed that, the pyridaben is able to induce DNA damage and chromatin abnormalities in spermatozoa which were evident by low IVF rate.This article extracted from Ph.D. thesis. (Ghodrat Ebadi Mans)

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Abstract: Among CMA3, aniline blue, SDS test, and acridine orange, CMA3 was the most sensitive and specific test that can be used along with routine semen analysis for more precise prediction of fertilization rate.

Cited by 83 publications

References 39 publications

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

PROTAMINE1 and PROTAMINE2 genes expression in the sperms of oligoasthenospermic individuals and intrauterine insemination candidates couples: Is there any significant differences?

Protective effect of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats

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