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Sedra, M.H.; Lebailly, Philippe; Trouslot, Pierre; Combes, Marie‐Christine; Hamon, Serge

doi:10.1023/a:1018377827903

Cited by 119 publications

(13 citation statements)

References 16 publications

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“…Data based on molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Restriction Fragment Length Polymorphism (RFLPs), have been used to characterize date palm genotypes. RAPD fingerprints have been used for identifying date palm accessions in Saudi Arabia (Al-Khalifah and Askari 2003), Egypt (Soliman et al 2003; Adawy et al 2005), Tunisia (Trifi et al 2000) and Morocco (Sedra et al 1998). Nevertheless these markers were not always successful in identification of date palm cultivars.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Libyan date palm resources by microsatellite markers

et al. 2013

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Molecular typing of 377 female date palm trees belonging to 18 Libyan cultivars and representing common genotypes in the central Libyan oasis of Al Jufrah was performed using 16 highly polymorphic microsatellite or SSR loci. A total of 110 alleles with an average of 6.88 alleles per locus were scored indicating the high level of polymorphism existing among the cultivars thus allowing their genetic fingerprinting. Moreover 28 alleles out of 110 were fixed. All the cultivars were characterized by negative values of the Fixation Index (F) due to an excess of heterozygotes with respect to HW equilibrium. The pattern of genetic diversity among cultivars was estimated by codominant genetic distances and presented by principal coordinates analysis (PCoA). The observed pattern evidences the genetic diversity existing among cultivars that allow distinguishing them easily. The average dissimilarity internal to each cultivar ranged from 0 to 21. Seven cultivars showed value zero indicating no genetic difference within cultivar in agreement with their Fixation Index (F = 1). A varietal identification key was also built using multiloci genotyping with only three microsatellite loci that identified 23 alleles in total. The possibility to attribute the unknown male plant to a cultivar was also considered and male parentage analysis was performed. Fifty-five male plants out of 63 were assigned to a definite cultivar with high confidence level. The positive result obtained in identifying males confirmed the suitability of SSR for clone fingerprinting and cultivar identification, thus opening new prospects for date palm breeding.

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Section: Introductionmentioning

confidence: 99%

Genetic characterization of Libyan date palm resources by microsatellite markers

et al. 2013

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“…The advent of molecular biology techniques has provided DNA - based markers for detection of genetic polymorphism in the plant germplasm and also those arise de novo due to mutation and somaclonal variation. Many studies report the use of molecular markers to study the genetic diversity and genetic relationship in date palm, including randomly amplified polymorphic DNAs (RAPDs) [5-10], Amplified fragment length polymorphic (AFLP) [11-13], and simple sequence repeat (SSR) [14-17]. …”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of gene-based SSR markers in date palm (Phoenix dactylifera L.)

et al. 2012

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BackgroundDate palm (Phoenix dactylifera L.) is an important tree in the Middle East and North Africa due to the nutritional value of its fruit. Molecular Breeding would accelerate genetic improvement of fruit tree through marker assisted selection. However, the lack of molecular markers in date palm restricts the application of molecular breeding.ResultsIn this study, we analyzed 28,889 EST sequences from the date palm genome database to identify simple-sequence repeats (SSRs) and to develop gene-based markers, i.e. expressed sequence tag-SSRs (EST-SSRs). We identified 4,609 ESTs as containing SSRs, among which, trinucleotide motifs (69.7%) were the most common, followed by tetranucleotide (10.4%) and dinucleotide motifs (9.6%). The motif AG (85.7%) was most abundant in dinucleotides, while motifs AGG (26.8%), AAG (19.3%), and AGC (16.1%) were most common among trinucleotides. A total of 4,967 primer pairs were designed for EST-SSR markers from the computational data. In a follow up laboratory study, we tested a sample of 20 random selected primer pairs for amplification and polymorphism detection using genomic DNA from date palm cultivars. Nearly one-third of these primer pairs detected DNA polymorphism to differentiate the twelve date palm cultivars used. Functional categorization of EST sequences containing SSRs revealed that 3,108 (67.4%) of such ESTs had homology with known proteins.ConclusionDate palm EST sequences exhibits a good resource for developing gene-based markers. These genic markers identified in our study may provide a valuable genetic and genomic tool for further genetic research and varietal development in date palm, such as diversity study, QTL mapping, and molecular breeding.

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“…Several marker systems have been used to study the genetic diversity of date palm. In brief, randomly amplified polymorphic DNA (RAPD) fingerprints have been used to identify date palm accessions in Algeria (Benkhalifa 1999), in Morocco (Sedra et al 1998), in Tunisia (Trifi et al 2000), in Saudi Arabia (Al-Khalifah and Askari 2003), and in Egypt (Soliman et al 2003; Adawy et al 2006). Amplified fragment length polymorphic (AFLP) markers have been applied to study the genetic diversity of date palm cultivars in Egypt and California (Cao and Chao 2002; El-Assar et al 2005; Adawy et al 2006).…”

Section: Introductionmentioning

confidence: 99%

New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)

et al. 2011

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New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and the remaining ten primers (33.4%) generated polymorphic banding patterns. A total of 77 alleles have been observed with a mean of 7.7 alleles per locus. The average of gene diversity was 0.80 ranging from 0.6 (in marker DP168) to 0.9 (in two markers DP157 and DP175). These new co-dominant markers will be a starting point for researchers making use of the markers for genetic mapping and diversity analysis of date palm.

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Cited by 119 publications

References 16 publications

Genetic characterization of Libyan date palm resources by microsatellite markers

Genetic characterization of Libyan date palm resources by microsatellite markers

Identification and characterization of gene-based SSR markers in date palm (Phoenix dactylifera L.)

New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)

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