Untitled

Abstract

The Hemagglutinin-Neuraminidase (HN) from the LPMV strain of Porcine rubulavirus was purified from virions by ultracentrifugation in a continuous 20-60% sucrose gradient and by ion exchange chromatography. The HN is a glycoprotein of 66 kDa constituted by 50.5, 13.3 and 13.6% of non polar, uncharged polar, and charged polar amino acids, respectively. The HN contains 4% of carbohydrates, its glycannic portion is constituted by Man, Gal, GlcNAc, GalNAc, and Neu5Ac in 3:3:4:1:1 molar ratios. The HN possesses hemagglutinating activity in the presence of erythrocytes from several animal species, including human ABO, and treating the erythrocytes with neuraminidase or pronase abolishes this activity. The binding specificity of the purified HN was determined by hapten inhibition assays, indicating that the hemagglutinating activity of the HN is specific for sialic acid and Neu5Acalpha2,3Gal-containing structures.

Boltzmann Model Predicts Glycan Structures from Lectin Binding

“…Terminal Neuα3Gal, for which GBPs have been reported but were absent in our data set. − By contrast, Neuα6Gal is one of the most sharply identified motifs in our data set (Figure S1), demonstrating one of the key advantages of GBPs: bond specificity.…”

“…We observed, however, that there are also terminal motifs that were less well-identified, and finding GBPs that recognize these features could improve the performance of our model in future studies. The following is a list of motifs that we believe may benefit from the identification of new partner GBPs: Terminal Neuα3Gal, for which GBPs have been reported but were absent in our data set. − By contrast, Neuα6Gal is one of the most sharply identified motifs in our data set (Figure S1), demonstrating one of the key advantages of GBPs: bond specificity. Terminal Fucα2Galβ, which is recognized in some contexts (i.e., BgH) but not in others. Internal polyLacNAc, which is recognized by several GBPs but not reliably. …”

A Boltzmann model predicts glycan structures from lectin binding

“…Terminal Neu α 3Gal, for which GBPs have been reported but were absent in our dataset. 43–48 By contrast, Neu α 6Gal, is one of the most sharply identified motifs in our dataset (Figure S-3), demonstrating one of the key advantages of GBPs: bond specificity .…”

“…The following is a list of motifs that we believe may benefit from identification of new partner GBPs: Terminal Neu α 3Gal, for which GBPs have been reported but were absent in our dataset. 43–48 By contrast, Neu α 6Gal, is one of the most sharply identified motifs in our dataset (Figure S-3), demonstrating one of the key advantages of GBPs: bond specificity . Terminal Fuc α 2Gal β , which is recognized in some contexts (i.e. BgH) but not in others. Internal polyLacNAc, which is recognized by several GBPs, but not reliably. As noted, some motifs can already be recognized by commercially-available lectins absent in our data.…”

La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation

“…The genome contains 6 genes encoding at least 9 proteins: the nucleoprotein (NP), matrix (M), fusion (F), hemagglutinin-neuraminidase (HN), large protein (L) and phosphoprotein (P) (Berg et al, 1997; Berg et al, 1991; Cuevas-Romero et al, 2013; Linne et al, 1992; Reyes-Leyva et al, 1999; Sanchez-Betancourt et al, 2012; Sundqvist et al, 1990; Svenda et al, 1997; Zenteno-Cuevas et al, 2007). The P gene of the paramyxoviruses encode several proteins by using different initiation codons and by a unique mechanism involving RNA editing that generates alternate mRNAs by site-specific co-transcriptional insertion of non-templated nucleotides (Hausmann et al, 1999a; Hausmann et al, 1999b; Iseni et al, 2002; Kolakofsky et al, 2005; Thomas et al, 1988).…”