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Abstract

This paper aims to point-out the effects of applying different live feed diets to the long-snouted seahorse (Hippocampus guttulatus Cuvier, 1829) of the Romanian Black Sea coast. The response of seahorses to Brachionus plicatilis-based, Artemia salina-based and combined diet is analysed, from the development, ethologic and biochemical point of view. Three experimental tanks were set in laboratory conditions, for a 10 day period. The different batches of seahorses collected from the natural environment were placed in the three tanks, after previous acclimation. Subsequently, one batch (Tank A) was fed exclusively with B. plicatilis, one with A. salina (Tank B), and one with a 50%/50% combined mixture of the two invertebrates (Tank C). The results obtained indicated a linear length and weight increase of H. guttulatus in all three feeding regimes, the final length (+10 days) being higher than the +5 days length and initial length. The biochemical composition of the three batches showed some variations. From the ethologic point of view, differences in the behavior of H. guttulatus in the three tanks were observed. Future research is required, focusing mainly on extending the experimental period (more than 30 days), separating males and females and diversifying the prey fed to seahorses.

Pollutant bioaccumulation in anchovy (Engraulis encrasicolus) tissue, fish species of commercial interest at the Romanian Black Sea coast

“…The extraction of OCPs and PCBs from biota samples was done with 30 ml acetone/hexane (1:1, v:v), in microwave extraction system Start E Milestone for 30 min at 120 o C. Further processing of the samples followed the steps: concentration of the extracts to rotary evaporator, clean-up on florisil, respectively, alumina/silica column and concentration of the samples using the Kuderna-Denish concentrator and nitrogen flow. The analytical determination of the OCPs and PCBs [10,11] content was made by the gas-chromatographic method with a Perkin Elmer gas chromatograph CLARUS 500, equipped with electron capture detector. The analyzed compounds were HCB, lindane, heptachlor, aldrin, dieldrin, endrin, p,p′-DDE (dichlorodiphenyldichloroethylene), p,p′-DDD (dichlorodiphenyldichloroethane) and p,p′-DDT (dichlorodiphenyltrichloroethane), PCB 28, PCB 52, PCB 101, PCB 138, PCB 118, PCB 153 and PCB 180.…”