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Abstract

ABSTRACT:Medicinal plants continue to be an important therapeutic aid for alleviating the ailments of human kind. The plant Dalbergia sissoo contains various chemical constituent with specific value in ethno-medicinal practices and has significance in the field of health care system. The main objective of this study involves the evaluation of anti-bacterial activity of D. sissoo leaves and barks extracts of chloroform, ethyl acetate, acetone and methanol. The assay was done by bioassay guided fractionation by disc diffusion method on both Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria using Ampicillin for S. aureus and Norfloxacin for E. coli as positive control and DMSO (Dimethyl Sulphoxide) as a negative control. The anti-bacterial activity of methanol extracts of leaves was found to be more as compared to other extracts. S. aureus was found to be more susceptible to plant extracts than E. coli. Thus, the results obtained confirm the therapeutic potency of D. sissoo used in traditional medicine.