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Günay, Deniz; Emiroğlu, Dilek; Tolon, Mustafa Tolga; Özden, Osman; Sayğı, Hülya

doi:10.4194/1303-2712-v15_2_41

Cited by 21 publications

(6 citation statements)

References 31 publications

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“…As expected, total protease activity changed with the water temperature in this study. It is reported that metabolism rate increased as the temperature raised and total protease enzyme activity increased at temperatures where the growth was optimum (Günay, Emiroğlu, Tolon, Özden, & Saygi, 2015). The lowest concentration levels belonged to winter which has the coldest water temperature.…”

Section: Discussionmentioning

confidence: 99%

Seasonal variations of digestive enzymes in sea cucumbers (Holothuria tubulosa, G. 1788) under culture conditions

2019

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Sea cucumber, Holothuria tubulosa (Gmelin, 1788), is an economically valuable species due to its rich nutrients content that being exported in Turkey. However, culture of this species is difficult due to a lack of knowledge. The main objective of this study is to investigate seasonal activities of main digestive enzymes (protease, lipase, and amylase) for nutritional requirements. In this sense, sea cucumbers were stocked in the glass aquarium and sediment was 10 cm. It was replaced monthly and enriched (1% of the living individual weight) by dried Sargassum sp. Total protease and amylase enzymes have shown higher activity compared to lipase. All three enzyme activities were maximum in the summer and minimum in the winter. In summer, the highest measured total protease, amylase, and lipase activity values were 6.45 ± 0.66, 6.77 ± 0.72, and 2.78 ± 0.32 U/mg per protein, respectively. In winter, the lowest total protease, amylase, and lipase activity values were measured as 2.03 ± 0.16, 1.14 ± 0.14, and 0.12 ± 0.01 U/mg per protein, respectively. As a conclusion of the study, seasonal expression of the main digestive enzymes was strictly dependent on water temperatures and food abundance and also it was ideal to feed this species with food containing high protein and carbohydrate under appropriate temperatures for commercial culture.

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Section: Discussionmentioning

confidence: 99%

Seasonal variations of digestive enzymes in sea cucumbers (Holothuria tubulosa, G. 1788) under culture conditions

2019

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“…Sicuro and Levine (2011), reported that H. tubulosa might be one of the potential aquaculture species in the Mediterranean Sea. Recent studies on adaptation of H. tubulosa to aquaculture reported successful results (Günay et al, 2015;Tolon et al 2015). However, studies on the hatching stages are lacking in the literature.…”

Section: Introductionmentioning

confidence: 99%

Effects of various stocking densities on hatching and survival rates of sea cucumber Holothuria tubulosa eggs (Gmelin, 1788)

Günay

Tolon

Emiroğlu

2018

EgeJFAS

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In this study, it was aimed to determine the opening and survival rates of Holothuria tubulosa eggs at four different stocking densities (1,5,15,30 eggs / ml) and to determine the effect of stocking density on egg size and larval size in different stages. Fertilized Holothuria tubulosa eggs were stocked in cylindrical conical collectors with a capacity of 30 l. Eggs were counted under light microscope at fertilization, early gastrulation, late gastrulation and hatching stages. It was found that there is an inverse relationship between stock intensity and opening rate (r = -0.848; p<0.001). The lowest hatching and survival rate was determined in 30 eggs / ml group (p <0.05). However, different stocking densities did not affect the diameter of the egg in different embryological stages (p> 0.05) and the length of early auricularia larva at 65 h (p> 0.05). As a result, the optimal stocking density for the highest hatching and survival rates of the fertilized eggs of Holothuria tubulosa was determined as 1-5 eggs / ml. Öz: Bu çalışmada, Holothuria tubulosa yumurtalarının, dört farklı stok yoğunluğunda (1,5,15,30 yumurta/ml) açılma ve yaşama oranlarını belirlemek ve aynı zamanda stok yoğunluğunun farklı evrelerdeki yumurta çapına ve larval boyutuna etkisini ortaya koymak amaçlanmıştır. Döllenmiş Holothuria tubulosa yumurtaları, 30 l hacimli silindir konik kollektörlere stoklanmıştır. Yumurtalar; döllenme anında, erken gastrulasyon, geç gastrulasyon ve yumurtadan çıkma aşamasında mikroskop altında sayılmıştır. Stok yoğunluğu ile açılma oranı arasında ters ilişki olduğu bulunmuştur (r = -0.848; p<0.001). En düşük açılma ve yaşama oranı 30 yumurta/ml stoklandığı deneme grubunda saptanmıştır (p<0.05). Ancak farklı stok yoğunlukları farklı embriyolojik safhalardaki yumurtanın çapını (p>0.05) ve 65. saatteki erken auricularia larvasının boyunu (p>0.05) etkilememiştir. Sonuç olarak, Holothuria tubulosa'nın döllenmiş yumurtalarının en yüksek açılma ve yaşama oranları için optimum stoklama yoğunluğu 1-5 yumurta / ml olmuştur.Anahtar kelimeler: Döllenmiş yumurta, açılma oranı, Holothuria tubulosa, deniz hıyarı, stok yoğunluğu, yaşama oranı

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“…Nonetheless, reproduction in both species occurs mainly in warmer months, from May to September (Despalotovic et al 2004;Aydın 2008;Aydın & Erkan 2015;Dereli et al 2016). In this study, water temperature of sampling areas can reach 25 o C during the reproduction period (TSMS 2020), and this may accelerate changes in biochemical and physiological characteristics of the species as a result of increasing food availability and maturity of sea cucumbers (Coulon & Jangoux 1993;Kazanidis 2010;Günay et al 2015;Künili & Colakoglu 2019). Therefore, the effect of reproduction on collagen yields in both species was determined by performing sampling until sufficient numbers of both immature and mature specimens in different length frequencies were obtained, and biochemical changes along with collagen yields could be compared regardless of temperature (Figs 5,6,7b).…”

Section: Discussionmentioning

confidence: 77%

Effects of morphometric and biochemical parameters on collagen and pepsin-solubilized collagen yields of Holothuria tubulosa (Gmelin, 1790) and Holothuria (Roweothuria) poli (Delle Chiaje, 1823)

Künili

2022

OandHS

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Due to their unique biochemical composition, sea cucumbers are highly prized marine echinoderm species. One of their most important properties is that they contain a high amount of collagen in their body wall. In this study, the relationship between collagen and pepsin-solubilized collagen yields from Holothuria tubulosa and Holothuria poli and morphometric and biochemical parameters were investigated. Collagen yields were in the range of 10.63–16.04% for H. tubulosa and 7.12–13.10% for H. poli. It was determined that they may be related to length, body wall weight, and biochemical composition at different length frequencies. Moreover, maturity may have a direct effect on the yield, as mature specimens were found to have lower content of collagen, whereas immature small specimens contained a higher percentage of collagen. It was found that with increasing pepsin concentration, the PSC yield increased to 1.83–1.89% in H. tubulosa and H. poli, respectively. It was determined that collagen from smaller individuals, which contained more moisture and ash, was likely more susceptible to pepsin hydrolyzation. This is the first published study demonstrating that collagen yield of sea cucumbers can vary with length, weight, maturity, and biochemical composition, in addition to species-specific differences.

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Cited by 21 publications

References 31 publications

Seasonal variations of digestive enzymes in sea cucumbers (Holothuria tubulosa, G. 1788) under culture conditions

Seasonal variations of digestive enzymes in sea cucumbers (Holothuria tubulosa, G. 1788) under culture conditions

Effects of various stocking densities on hatching and survival rates of sea cucumber Holothuria tubulosa eggs (Gmelin, 1788)

Effects of morphometric and biochemical parameters on collagen and pepsin-solubilized collagen yields of Holothuria tubulosa (Gmelin, 1790) and Holothuria (Roweothuria) poli (Delle Chiaje, 1823)

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