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Eckey-Kaltenbach, Heidrun; Kiefer, Evi; Grosskopf, E.; Ernst, D.; Sandermann, Heinrich

doi:10.1023/a:1005786317975

Cited by 63 publications

(4 citation statements)

References 38 publications

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“…The expression levels of HSPs (IPR008978 and IPR002068) in terms of RPKM for the induced library were 58,328.8 and 12,510.56 (vs. 140.4 and 212.3, for the repressed library respectively), which clearly indicate a strong induction of HSPs in O 3 -treated plants [log 2 fold 8.7 and 5.9, respectively (Table 2 )]. The observed results are in accordance with previous observations reported by Eckey-Kaltenbach et al ( 1997 ) in Petroselinum crispum seedlings exposed to O 3 (200 ppb, 10 h) and post-cultivated in pollutant-free air, using a Northern blot analysis. Recently, Al-Whaibi ( 2011 ) reviewed the role of HSPs demonstrating their function as molecular chaperones, regulating the (i) localization, (ii) degradation, (iii) accumulation, and (iv) folding of proteins during their synthesis.…”

Section: Resultssupporting

confidence: 93%

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

et al. 2016

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Tropospheric ozone (O3) is a global air pollutant that causes high economic damages by decreasing plant productivity. It enters the leaves through the stomata, generates reactive oxygen species, which subsequent decrease in photosynthesis, plant growth, and biomass accumulation. In order to identify genes that are important for conferring O3 tolerance or sensitivity to plants, a suppression subtractive hybridization analysis was performed on the very sensitive woody shrub, Viburnum lantana, exposed to chronic O3 treatment (60 ppb, 5 h d−1 for 45 consecutive days). Transcript profiling and relative expression assessment were carried out in asymptomatic leaves, after 15 days of O3 exposure. At the end of the experiment symptoms were observed on all treated leaves and plants, with an injured leaf area per plant accounting for 16.7% of the total surface. Cloned genes were sequenced by 454-pyrosequencing and transcript profiling and relative expression assessment were carried out on sequenced reads. A total of 38,800 and 12,495 high quality reads obtained in control and O3-treated libraries, respectively (average length of 319 ± 156.7 and 255 ± 107.4 bp). The Ensembl transcriptome yielded a total of 1241 unigenes with a total sequence length of 389,126 bp and an average length size of 389 bp (guanine-cytosine content = 49.9%). mRNA abundance was measured by reads per kilobase per million and 41 and 37 ensembl unigenes showed up- and down-regulation respectively. Unigenes functionally associated to photosynthesis and carbon utilization were repressed, demonstrating the deleterious effect of O3 exposure. Unigenes functionally associated to heat-shock proteins and glutathione were concurrently induced, suggesting the role of thylakoid-localized proteins and antioxidant-detoxification pathways as an effective strategy for responding to O3. Gene Ontology analysis documented a differential expression of co-regulated transcripts for several functional categories, including specific transcription factors (MYB and WRKY). This study demonstrates that a complex sequence of events takes place in the cells at intracellular and membrane level following O3 exposure and elucidates the effects of this oxidative stress on the transcriptional machinery of the non-model plant species V. lantana, with the final aim to provide the molecular supportive knowledge for the use of this plant as O3-bioindicator.

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Section: Resultssupporting

confidence: 93%

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

et al. 2016

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“…The enrichment of genes which related to ‘pathogenesis’ indicated the similarity between plant response to O 3 and pathogen infection, as both of these stresses triggered the production of ROS [ 24 ]. This result is consistent with the other reports in which pathogenesis-related (PR) proteins were induced by O 3 [ 13 , 25 – 27 ].…”

Section: Discussionsupporting

confidence: 93%

Transcriptomic analysis of Pak Choi under acute ozone exposure revealed regulatory mechanism against ozone stress

Zhang

et al. 2017

BMC Plant Biol

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BackgroundGround-level ozone (O3) is one of the major air pollutants, which cause oxidative injury to plants. The physiological and biochemical mechanisms underlying the responses of plants to O3 stress have been well investigated. However, there are limited reports about the molecular basis of plant responses to O3. In this study, a comparative transcriptomic analysis of Pak Choi (Brassica campestris ssp. chinensis) exposed to different O3 concentrations was conducted for the first time.ResultsSeedlings of Pak Choi with five leaves were exposed to non-filtered air (NF, 31 ppb) or elevated O3 (E-O3, 252 ppb) for 2 days (8 h per day, from 9:00–17:00). Compared with plants in the NF, a total of 675 differentially expressed genes (DEGs) were identified in plants under E-O3, including 219 DEGs with decreased expressions and 456 DEGs with increased expressions. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that O3 stress invoked multiple cellular defense pathways to mitigate the impaired cellular integrity and metabolism, including ‘glutathione metabolism’, ‘phenylpropanoid biosynthesis’, ‘sulfur metabolism’, ‘glucosinolate biosynthesis’, ‘cutin, suberine and wax biosynthesis’ and others. Transcription factors potentially involved in this cellular regulation were also found, such as AP2-ERF, WRKY, JAZ, MYB etc. Based on the RNA-Seq data and previous studies, a working model was proposed integrating O3 caused reactive oxygen burst, oxidation-reduction regulation, jasmonic acid and downstream functional genes for the regulation of cellular homeostasis after acute O3 stress.ConclusionThe present results provide a valuable insight into the molecular responses of Pak Choi to acute O3 stress and the specific DEGs revealed in this study could be used for further functional identification of key allelic genes determining the O3 sensitivity of Pak Choi.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1202-4) contains supplementary material, which is available to authorized users.

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“…Plants respond to ozone stress by inducing a variety of defense responses. Active defense mechanisms include an increase in phenylpropanoid pathway gene transcripts, such as those encoding PAL and CHS ( Eckey-Kaltenbach et al., 1997 ; Pääkkönen et al., 1998b ). When plants are exposed to low levels of ozone, physiological and metabolic changes are observed.…”

Section: Discussionmentioning

confidence: 99%

Ozone control as a novel method to improve health-promoting bioactive compounds in red leaf lettuce (Lactuca sativa L.)

Lee

Gotō

2022

Front. Plant Sci.

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In this study, we determined the short-term effects of ozone exposure on the growth and accumulation of bioactive compounds in red lettuce leaves grown in a controlled environment plant factory with artificial light, also known as a vertical farm. During cultivation, twenty-day-old lettuce (Lactuca sativa L. var. Redfire) seedlings were exposed to 100 and 200 ppb of ozone concentrations for 72 h. To find out how plants react to ozone and light, complex treatments were done with light and ozone concentrations (100 ppb; 16 h and 200 ppb; 24 h). Ozone treatment with 100 ppb did not show any significant difference in shoot fresh weight compared to that of the control, but the plants exposed to the 200 ppb treatment showed a significant reduction in fresh weight by 1.3 fold compared to the control. The expression of most genes in lettuce plants exposed to 100 and 200 ppb of ozone increased rapidly after 0.5 h and showed a decreasing trend after reaching a peak. Even when exposed to a uniform ozone concentration, the pattern of accumulating bioactive compounds such as total phenolics, antioxidant capacity and total flavonoids varied based on leaf age. At a concentration of 200 ppb, a greater accumulation was found in the third (older) leaf than in the fourth leaf (younger). The anthocyanin of lettuce plants subjected to 100 and 200 ppb concentrations increased continuously for 48 h. Our results suggest that ozone control is a novel method that can effectively increase the accumulation of bioactive compounds in lettuce in a plant factory.

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Untitled

Cited by 63 publications

References 38 publications

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

Transcriptomic analysis of Pak Choi under acute ozone exposure revealed regulatory mechanism against ozone stress

Ozone control as a novel method to improve health-promoting bioactive compounds in red leaf lettuce (Lactuca sativa L.)

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