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Abstract

The present study estimated the antidiabetic activity of methanolic extract of plant fruits of plant Withania coagulans L. Dunal. Withania coagulans (family Solanaceae) is commonly known as "Paneer ke phool" in Hindi and "Indian cheese maker" or "Vegetable rennet" in English, distributed in drier parts of India. The primary aim of this study was to confirm antidiabetic action of Withania coagulans by using the invitro method of glucose uptake by yeast cells using methanolic fruit extract of the sample. It is reported that the Withanolides are responsible for its anti-hyperglycemic activity, which has been standardized by the TLC method using 50% Sulphuric acid and dragendroff's reagents as a spraying agent in this study. The current research found that Withania coagulans methanolic fruit extract improved glucose absorption in yeast cells by 0-90% at various glucose concentrations (5, 10, and 25 mM). Metformin was used as a standard reference. At all glucose concentrations, the highest concentration of WCME (5 mg/ml WCME) demonstrated maximum activity and showed the greatest increase (81.83 percent) in the presence of 25 mM glucose. In conclusion, this report suggests that the increase of glucose transport across the cell membrane might be the anti-hyperglycaemic mechanism of action of Withania coagulans fruits. INTRODUCTION:Traditional medicine is "the knowledge, skills, and practices based on the theories, beliefs, and experiences indigenous to different cultures, used in the maintenance of health and in the prevention, diagnosis, improvement or treatment of physical and mental illness. The vast use of Traditional medicine may be due to many reasons like high cost and long-time taken to develop the new drug, toxicity, and non-renewable source of basic raw materials.