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Gupta, P. K.; Varshney, Rajeev K.

doi:10.1023/a:1003910819967

Cited by 701 publications

(126 citation statements)

References 187 publications

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“…RAPD markers are used to detect DNA polymorphism without requirement of previous knowledge of the target genome (Williams et al, 1990). Microsatellite markers are also markers of choice (Esselink et al, 2003) because they are abundant, uniformly distributed, highly polymorphic, codominant, rapidly produced by PCR and easily accessed through published primer sequences (Gupta and Varshney, 2000).…”

Section: Introductionmentioning

confidence: 99%

ASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (<i>ROSA SP</i>.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERS

El-Assal

El-Awady

El-Tarras

et al. 2014

American Journal of Biochemistry and Biotechnology

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Taif-roses are a famous rose type that cultivated in Taif region and well known with their deep and intensive fragrance in the Arabian World. Despite of the great economical importance of Taif-roses for the kingdom of Saudi Arabia, their genetic origin has not been yet elucidated. The present study was mainly aimed to assess the genetic relationship between Taif-roses and some rose genotypes that grown in some kingdom neighboring countries using molecular markers and aromatic amino acids contents. Three Taif-roses genotypes namely Hada, Shafa-1 and Shafa-2 were compared to nine different rose genotypes that are grown in Egypt and Syria. Out of 12 RAPD, 8 ISSR and 8 SSR primers used, clear and repeatable band profile of 8, 6 and 7 primers was obtained from the three markers, respectively. Total of 111, 64 and 15 bands with polymorohism of 96.4, 90.6 and 93.3% were obtained using RAPD, ISSR and SSR, respectively. The discriminating power of the three markers has led to efficient grouping of the 12 rose genotypes using Unweighted Pair Group Method (UPGMA). Among the 12 genotypes, Syrian-Gory rose shown the highest genetic similarity of 75, 92 and 65% with the three Taif-roses genotypes Hada, Shafa-1 and Shafa-2, respectively. The established dendrogram was clearly separated the 12 rose genotypes into four major groups in which the three Taif-roses genotypes were clustered in the same group with the Gory rose-Syrian genotype. Moreover, the data revealed that among the studied rose genotypes, the contents of aromatic amino acids in Syrian-Gory rose and the Taif rose-Hada was the highest and followed by the Egyptian Balady rose 1. While Dutch rose 1, 2, 3 and Dutch tulip 1, 2 were recorded to be the lowest. Together, these results indicate that Taif-rose has closed genetic relations to the Gory rose-Syrian cultivated in Syria. Additionally, a reproducible protocol for In vitro propagation, of Taif-rose genotype (Hada) was developed.

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Section: Introductionmentioning

confidence: 99%

ASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (<i>ROSA SP</i>.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERS

El-Assal

El-Awady

El-Tarras

et al. 2014

American Journal of Biochemistry and Biotechnology

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“…Although some of these marker types are very similar (e.g., ASAP, ASO and AS-PCR), some synonymous (e.g., ISSR, RAMP, RAM, SPAR, AMP-PCR, MP-PCR, and ASSR), and some identical (e.g., SSLP, STMS, STR and SSR), there are still a wide range of techniques for researchers to choose upon [14]. Restriction fragment length polymorphisms (RFLPs) are reliable and yield co-dominant data, but are also timeconsuming and expensive, requiring relatively large amount of highly purified DNA and they do not lend themselves to automation [28]. Random amplified polymorphic DNA (RAPD) markers are unreliable with poor replication success among laboratories [3], [9].…”

Section: The Use Of Biomarkers In Quantitative Traits Selectionmentioning

confidence: 99%

“…Cost: The marker assay must be cost-effective in order for MAS to be feasible. The most widely used markers in major cereals are called simple sequence repeats (SSRs) or microsatellites [28], [29]. They are highly reliable (i.e.…”

Section: The Use Of Biomarkers In Quantitative Traits Selectionmentioning

confidence: 99%

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Molecular marker assisted selection as approach to increase the selection efficiency of drought tolerant genotypes

Shiri¹,

Akhavan²

2014

International Journal of Scientific World

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Identifying the complete-linked molecular markers with target gene and mapping its chromosome locus is an important goal in plant breeding for gene cloning and marker-aided selection. Due to complexity of the interactions, in most of the agronomic traits, especially the interaction between the grain yield and the environmental factors, classic methods do not function appropriately in improving agronomic traits at present. If the selection is made based on genotype by DNA markers, the efficiency of selection will increase considerably. In a genetic evaluation program, the combination between the data from the linkage between marker position and quantitative traits loci (QTL) as well as the phenotypic data can be used to increase the accuracy of the assessments and thereby the accuracy of selection. The selection in which inherited values are used along with the marker data in selection of superior genotypes in a breeding program is called Marker-assisted-selection (MAS).

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“…SSR markers are abundant and highly variable in the plant genome (Matsuoka et al, 2002). SSRs are multi-allelic, highly polymorphic, and co-dominant in nature, which makes them one of the most useful genetic markers for breeders in molecular analysis of crops (Gupta and Varshney, 2000). SSRs have proven to be a valuable tool for genomic mapping, population and conservation genetics studies, property right protection, marker-assisted selection, and diversity measurements in maize (Warburton et al, 2002;Pinto et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers

Farooqi¹,

J²,

Hong

et al. 2016

Genet. Mol. Res.

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ABSTRACT. In this study, eight inbred maize lines with high or low tolerance to cold temperature were investigated using bulk segregant analysis (BSA). Genetic diversity and genetic relationships were investigated using 100 simple sequence repeat (SSR) markers linked to cold tolerance in maize. A total of 319 alleles were identified. Among these, 128 were high coldtolerant-specific alleles and 61 were poorly coldtolerant-specific alleles, while the remaining 130 were shared between the two types. The number of alleles per locus ranged from 2 to 5, with an average of 3.19. The major allele frequency varied from 0.39 to 0.53 with an average of 0.47. The average gene diversity and polymorphic information content among all lines were 0.63 and 0.58, respectively. A dendrogram analysis identified three main clusters and most of the high tolerant inbred lines were clearly distinguished from the poorly tolerant inbred lines. In addition to the BSA, a total of 84 SSR markers were identified as high cold tolerance-specific alleles and 52 SSRs were detected as poorly cold tolerance-specific alleles. Of these, bnlg1273, umc1124, dupssr21, mmc0251, mmc0181, and phi041 have great potential for being molecular markers for cold tolerance in maize. Our results were in agreement with results previously reported for SSR markers linked with cold tolerance in maize. The identification and characterization of high and poorly cold tolerant maize lines based on SSR markers will be useful for future maize breeding studies.

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Cited by 701 publications

References 187 publications

ASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (<i>ROSA SP</i>.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERS

ASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (<i>ROSA SP</i>.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERS

Molecular marker assisted selection as approach to increase the selection efficiency of drought tolerant genotypes

Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers

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