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Larionov, P. M.; Malov, Alexander N.; Mandrik, M. M.; Maslov, N. A.; Orishich, A. M.

doi:10.1023/a:1023212206592

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…Затем пробы инкубировали в течение 3 ч (время, достаточное для взаимодействия лекарства с клетками) в инкубаторе с 5% содержанием СО 2 при температуре 37  С (условия внутренней среды человека). Через 3 ч инкубации из каждой лунки отбирали 500 мкл надосадочной жидкости и в оставшиеся 500 мкл вносили флуоресцентные красители в конечной концентрации 1 нмоль/мкл для Сalcein АМ (компания Invitrogen, США), который окрашивает только жизнеспособные клетки и в конечной концентрации 2 нмоль/мкл для Ethidium bromide (компания Sigma-Aldrich, США), который окрашивает только мёртвые клетки [16]. Пробы снова ставили в термостат при тех же условиях ещё на 30 мин (время, достаточное для окрашивания клеток).…”

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Personalized trimetazidine prescription as a cytoprotective agent in patients with coronary artery disease

Ромащенко

2021

Russ J Cardiol

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Aim. To develop a personalized approach to the trimetazidine use in patients with coronary artery disease (CAD) based on the criteria for predicting the cytoprotective activity tested in vitro.Material and methods. We examined 30 patients with class I-III stable effort angina with concomitant hypertension and heart failure. The patients underwent echocardiography, complete blood count, biochemical tests with determination of the lipid profile, creatine phosphokinase (CPK), CPK-MB, renal and hepatic parameters. To determine the cytoprotective activity of trimetazidine, white blood cells (WBCs) of patients were examined in vitro using an Eclipse Ti-U inverted fluorescence microscope (Nikon, Japan). Living and dead cells were determined by staining WBCs with fluorescent dyes (Calcein AM, Ethidium bromide). Cell viability index (CVI) was calculated. The statistical processing was carried out. The criteria for predicting the trimetazidine cytoprotective effect were determined using Wald statistics.Results. When trimetazidine was injected into a WBC suspension sample, two types of cell viability changes were observed: in 60% of patients, CVI increased, on average, by 37% (from 23% to 60%, p<0,001) and in 40% of patients, CVI decreased, on average, by 30% (from 54% to 24%, p<0,05).A number of conditions of the patient initial status were identified for the manifestation of trimetazidine cytoprotective activity: grade 1 hypertension; right ventricular end diastolic dimension up to 30 mm according to echocardiography; normal lipid profile with a total cholesterol <5,3 mmol/L, very-low-density lipoproteins <1 mmol/L and an atherogenic coefficient up to 3 CU, myocyte and cardiomyocyte destruction (total CPK >100 U/L and CPK-MB >15 U/L), normal liver function (alanine aminotransferase <25 U/L), renal dysfunction (total protein <75 g/L, urea >8 mmol/L and blood creatinine >100 pmol/L), normal thrombopoiesis (immature platelet fraction <5%) and the state of functional adaptive system resistance (blood lymphocytes <30% and neutrophils >4x109/L).Conclusion. According to this in vitro analysis, the trimetazidine significantly increases (by an average of 37%) the cell (WBC) viability in 60% of patients with CAD. There are conditions of patient initial status, which specifies an individual pharmacodynamic target for the cytoprotective action of the drug.

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Section: материал и методыunclassified

Personalized trimetazidine prescription as a cytoprotective agent in patients with coronary artery disease

Ромащенко

2021

Russ J Cardiol

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“…This technique has also been demonstrated to be a useful tool for monitoring DNA damage and for cancer diagnostics [9][10][11]. Recently LIF has been used experimentally to analyze the viability of porcine hearts after perfusion with storage solutions [12].…”

Section: Introductionmentioning

confidence: 99%

Use of laser auto-fluorescence for evaluating liver grafts

et al. 2006

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There has been an increasing demand for liver transplantation, while the supply of liver grafts remains limited. Strategies to augment the donor pool include "liver-splitting", "domino" procedure, Living-related liver transplantation and the use of marginal donors. Simultaneously, there has been growing interest in Laser-induced fluorescence (LIF) as a diagnostic tool, mainly in oncology. The basis of all optical spectroscopic techniques is that physiological, morphological or biochemical changes associated with physical disorders, affect the interaction between light and tissue. The aim of this experimental study was to analyze the applicability of LIF in evaluating liver grafts, in rats. In this experiment, the animals were divided into two groups according to the temperature of the perfusion medium. The perfusion medium used was saline solution between 0-4• C in one group (CS group) and the same solution, between 22-26• C, in the other group (RT group). Mitochondrial respiratory capacity, swelling and membrane potential along with the cellular ATP content were used as the functional and metabolic indicators of viability. The Cluster-Russia fluorescence spectroscopy system was used to analyze the LIF. Deterioration of mitochondrial function and ATP content occurred progressively and relatively rapidly. LIF reflected the findings of the mitochondrial respiratory control ratio in the CS group, but not in the RT group. The ratio of the intensity of fluorescence detected at 636 nm and 600 nm showed rapid change 5 to 6 hours after perfusion in the CS group. Therefore LIF shows great potential as an auxiliary tool in the analysis of liver grafts, for transplantation. Graph comparing the reduction of the mitochondrial respiratory control ratio (RCR) and hepatic fluorescence in the CS group, during the 12 hours after perfusion

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Visualization of human heart conduction system by means of fluorescence spectroscopy

et al. 2011

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The conduction system of the heart is a specific muscular tissue, where a heartbeat signal originates and initiates the depolarization of the ventricles. The muscular origin makes it complicated to distinguish the conduction system from the surrounding tissues. A surgical intervention can lead to the accidental harm of the conduction system, which may eventually result in a dangerous obstruction of the heart functionality. Therefore, there is an immense necessity for developing a helpful method to visualize the conduction system during the operation time. The specimens for the spectroscopic studies were taken from nine diverse human hearts. The localization of distinct types of the tissue was preliminary marked by the pathologist and approved histologically after the spectral measurements. Variations in intensity, as well as in shape, were detected in autofluorescence spectra of different heart tissues. The most distinct differences were observed between the heart conduction system and the surrounding tissues under 330 and 380 nm excitation. The spectral region around 460 nm appeared to be the most suitable for an unambiguous differentiation of the human conduction system avoiding the absorption peak of blood. The visualization method, based on the intensity ratios calculated for two excitation wavelengths, was also demonstrated.

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Cited by 4 publications

References 4 publications

Personalized trimetazidine prescription as a cytoprotective agent in patients with coronary artery disease

Personalized trimetazidine prescription as a cytoprotective agent in patients with coronary artery disease

Use of laser auto-fluorescence for evaluating liver grafts

Visualization of human heart conduction system by means of fluorescence spectroscopy

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