Untitled

Abstract

Background: Olive leaf, a versatile plant obtained from Olea europaea which was enriched with various secondary metabolites likes polyphenols, secoirodiods eg. Oleuropein, Hydroxytyrosol etc. Current treatment modalities for meningitis were ended up with MDR. Hence, a new strategy is mandate to alleviate bacterial meningitis. Objective: Current research structured to identify the potential of Oleuropein loaded NLC in the inhibition of Bacterial Meningitis. Glycerol is used as bio-solvent in hot blanching assisted microwave extraction & extracts were analyzed by using UV, FTIR, HPTLC and HPLC to quantify the Oleuropein. Methodology: NLC was formulated by melt dispersion ultrasonication method using Box-behnken design. Formulated NLC was analyzed for particle size, zeta potential and in-vitro drug release studies. Toxicity and Histo-pathological examination was done in albino Wistar rats using cerebellar cistern puncture method to estimate In-vivo potential of Oleuropein in bacterial meningitis. Results and Discussion: From the data, it was observed that hot blanching process potentiates the extraction and the data depicts that aqueous glycerol extract contain huge amount of Oleuropein. Optimized NLC resides with 165nm of size, ZP of -40mv and PDI of 0.3 with a sustainable release of drug for 12hrs. By Histo-pathological examination, regeneration of brain tissue was initiated in the hippocampus region in treated group as compared to control group. Conclusion: Combination of hot blanching with microwave extraction for Oleuropein and subsequent loading of drug into NLC shown significant positive effect on treatment of Streptococcal meningitis.

Encapsulation of Oleuropein in Nanostructured Lipid Carriers: Biocompatibility and Antioxidant Efficacy in Lung Epithelial Cells

“…In light of the characterization results, NLC-OLE50 seemed to be a promising carrier for OLE with enhanced antioxidant activity and thus we continued to study its efficacy in lung epithelial cell models. Several authors have reported micro-and nanoencapsulation methods for olive leaf extracts but to the best of our knowledge, there is a lack of studies regarding the impact that the formulation process could have on the bioactivity of this polyphenol in a biological system [22,23,52].…”

“…In this context, micro and nanoencapsulation technologies have been proposed as promising strategies [20]. Currently, some encapsulation methods for Olea europaea leaf extracts have been described such as W/O nanoemulsions and W/O/W double emulsions [21][22][23], spray-drying [24,25], formation of inclusion complexes with cyclodextrin [26], electrostatic extrusion [27], biopolymer complexes/double emulsions [21], and liposomes [28]. However, they have some limitations such as the use of organic solvents, tedious and time consuming manufacturing processes, high particle sizes, and low yield (for spray drying).…”

Development of Computational In Silico Model for Nano Lipid Carrier Formulation of Curcumin

“…All measurements were made in triplicate at 25 °C. The zeta potential was then directly determined from the equation by using the Smolochowski equation as follows [ 80 , 81 ]. ζ = εµ/η where ζ is the zeta potential, µ is the electrophoretic mobility, ε is the electric permittivity of the liquid, and η is the viscosity of the liquid.…”