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Abstract

Complementary DNA clones representing the fusion (F) protein gene of the porcine rubulavirus LPMV were isolated and sequenced. The F gene was found to be 1,845 nucleotides long containing one long open reading frame capable of encoding a protein of 541 amino acids. The cleavage motif for F0 into F1 and F2 is His-Arg-Lys-Lys-Arg. A sequence comparison and a phylogenetic analysis was performed in order to identify possible functional domains of paramyxovirus fusion proteins and also to classify the porcine rubulavirus. The F gene of LPMV is most closely related to the human mumps virus and simian virus type 5 F genes, and is therefore classified into the rubulavirus genus. A coding region for a small hydrophobic protein was however not found between the F and hemagglutinin-neuraminidase (HN) genes as previously found in both SV5 and mumps.

Immunoinformatics approach for predicting epitopes in HN and F proteins of Porcine rubulavirus

“…None of the substitutions in the F protein cleavage site (HRKKR) have been found. Some reports suggest that the cleavage site in the F protein participates in the virulence of PRV [ 5 , 9 ]. There are been reports that the surface PRV proteins, HN and F, are the most antigenic components of other paramyxoviruses (e.g.…”

Molecular and epidemiological studies ofPorcine rubulavirusinfection – an overview

“…1 b). The P gene has the capacity to encode four possible polypeptides, P, V, C, and I, from the same gene via RNA editing and alternative initiation of translation; that is, the addition of one or two G residues at the editing site allows expression of the I or P protein, respectively ( 8 – 10 ). All viruses belonging to the families in the order Mononegavirales require that the virion contain its own RNA-dependent RNA polymerase, as the cells lack such enzyme, thus these viruses direct the synthesis of mRNA at the start of the infectious cycle.…”

“…Molecular studies of PorPV, with an emphasis on proteins of the replicative complex, suggest that this virus has existed as a separate species for a long time in nature and that it could have been transmitted from a natural wildlife reservoir to domesticated pigs ( 10 , 18 , 19 ). Early sequence analysis showed the relationship of PorPV to the MuV and the Simian virus 5 (SV5) ( 7 – 10 , 18 , 19 ), with an amino acid identity of approximately 40%. Recent phylogenetic analyses comparing the completed genome sequence and the genetic organisation indicate that PorPV is more closely related to Mapuera virus (MprPV) than to other members of the genus Rubulavirus , suggesting that PorPV may possibly originate from bats ( 20 ).…”

La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation

“…The genome contains 6 genes encoding at least 9 proteins: the nucleoprotein (NP), matrix (M), fusion (F), hemagglutinin-neuraminidase (HN), large protein (L) and phosphoprotein (P) (Berg et al, 1997; Berg et al, 1991; Cuevas-Romero et al, 2013; Linne et al, 1992; Reyes-Leyva et al, 1999; Sanchez-Betancourt et al, 2012; Sundqvist et al, 1990; Svenda et al, 1997; Zenteno-Cuevas et al, 2007). The P gene of the paramyxoviruses encode several proteins by using different initiation codons and by a unique mechanism involving RNA editing that generates alternate mRNAs by site-specific co-transcriptional insertion of non-templated nucleotides (Hausmann et al, 1999a; Hausmann et al, 1999b; Iseni et al, 2002; Kolakofsky et al, 2005; Thomas et al, 1988).…”