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Georgiev, Pavel; Muravyova, Ekaterina; Golovnin, Anton; Gracheva, Elena; Belenkaya, Tatiana

doi:10.1023/a:1009002820308

Cited by 4 publications

(3 citation statements)

References 36 publications

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Section: Resultsmentioning

confidence: 99%

“…1A ) was obtained by induction of P-M hybrid dysgenesis in a line containing the y 2 mutation [9]. Allele derivatives y +s – y 2s1 and y 2s2 containing a chromosome X region 1A duplication in the pre-promoter region of yellow have also been obtained [9, 10]. The study of the structure of the alleles y 2s1 and y 2s2 made it possible to identify the regulatory element 1A-RE, which activates long-distance yellow expression and is a yellow -specific insulator, within the duplicated fragment comprising the region 1A [10].…”

Section: Resultsmentioning

confidence: 99%

“…The chimeric element consists of 1,2 kb P -elements located “tail to tail” and a 3 030 bp sequence trapped between them, which presents a duplication of the region 1A of chromosome X and is located distal to the yellow locus in the genome ( Fig. 1A ) [9]. This duplication includes a fragment of the unexplored gene CG3777 , which is expressed at the same stages of development as the yellow gene.…”

Section: Resultsmentioning

confidence: 99%

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Mapping the D.melanogaster En1A Enhancer Modules Responsible for Transcription Activation and LongDistance Enhancer-Promoter Interactions

et al. 2017

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The structure of the new enhancer En1A of the 1A region of the X chromosome of D. melanogaster was investigated. Two distinct regulatory elements were found. The first element is responsible for transcription activation, and the second element provides specific interaction with the promoter of the yellow gene. The findings support the hypothesis of a modular structure for enhancers, including certain sequences that bind transcription activators and special communication elements providing long-distance enhancer-promoter interaction.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Mapping the D.melanogaster En1A Enhancer Modules Responsible for Transcription Activation and LongDistance Enhancer-Promoter Interactions

et al. 2017

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Individual and population variation in cercariae of bird schistosomes of the Trichobilharzia ocellata species group as revealed with the polymerase chain reaction

Semyenova¹,

Chrisanfova²,

Filippova³

et al. 2005

Russ J Genet

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The polymerase chain reaction with arbitrary (RAPD-PCR) or specific primers was used to study the population variation and to identify the species in cercariae of schistosomes of the Trichobilharzia ocellata species group (Trematoda, Schistosomatidae). In total, 28 cercariae were obtained from two spontaneously invaded mollusks Lymnaea stagnalis (LS) and L. ovata (LO), which were collected in different ponds of Moscow. RAPD-PCR was carried out with two arbitrary primers, OPA9 and OPB11, which each detected different levels of individual and among-group variation and revealed considerable genetic differentiation of cercariae from different host mollusks. To check whether the cercariae of the two samples belong to one species, sequencing was performed with a region corresponding to intergenic transcribed spacer 2 (ITS2), which was earlier proposed for cercaria identification in three European species of bird schistosomes of the genus Trichobilharzia ( T. franki, T. regenti , and T. szidati ). The ITS2 sequences of two LO cercariae were identical, each consisted of 319 bp, and showed 100% homology to the T. franki ITS2 sequence. The ITS2 sequences of two LS cercariae were identical, each consisted of 323 bp, and showed 99.4% homology to the T. szidati counterpart. The causes of genetic variation in cercariae and prospects of using RAPD markers to study different stages of the life cycle in trematodes are discussed. MOLECULAR GENETICS

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Roles of silencers from the regulatory region of the Abdominal-B gene and the Zw5 and Su(Hw) insulators in regulating gene expression in Drosophila melanogaster

Kullyev

Georgiev

et al. 2004

Russ J Genet

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The Abdominal-B ( Abd-B ) homeotic gene is responsible for the formation of the abdominal segments in Drosophila melanogaster. Its regulatory region of approximately 50 kb consists of chromatin domains, each controlling expression in a single segment [1, 2] (Fig. 1). The domains each contain at least one enhancer, which activates expression of the Abd-B gene in early embryo development, and a silencer, which maintains the expression pattern during development. Molecular genetic analysis showed that the Abd-B locus contains boundary elements MCP, Fab-7, and Fab-8, which ensure the functional independence of the regulatory domains of the Abd-B gene and act as insulators [3,4]. So-called Polycomb responsive elements (PREs) are located in the immediate vicinity of the insulator or within the insulator and play a role in gene silencing, which is essential for normal development. Enhancers that must be nonfunctional in a particular parasegment are inhibited by suppressor complexes assembled from Polycomb group proteins on PREs. Thus, MCP, Fab-7, and Fab-8 determine the boundary between active and inactive chromatin regions. In each chromatin domain, the enhancer is flanked by two insulators and is still capable of activating the promoter.There is evidence that two Su(Hw) insulators interact with each other and that their interaction either strengthens or neutralizes insulation [5,6]. These findings made it possible to advance a model that insulators located in the regulatory region of the Abd-B gene selectively interact with each other to ensure efficient communication between the active enhancer and the Abd-B promoter and, on the other hand, complete inactivation of the other enhancers [7]. Yet recent studies have shown that the Fab-7 and Fab-8 insulators do not interact with each other or with the Su(Hw) insulator [8]. However, regulatory elements containing the Fab-7 or Fab-8 insulator and the adjacent silencer do interact even when located on different chromosomes [4,9,10]. On the strength of these data, elements of the silencer can be assumed to ensure the long-distance interaction of insulators (Fig. 2b).To check this assumption, we obtained a genetic construct combining the Zw5 and Su(Hw) insulators and silencers, which were placed in the regulatory regions of the yellow and miniwhite genes (Fig. 2a). The efficiency of the interaction between the silencers was inferred from their ability to cooperatively suppress transcription of the yellow gene, which was located between the silencers, and to neutralize the effect of the Su(Hw) insulator. Experiments showed that the silencers were ineffective in suppressing transcription of the yellow gene or neutralizing the effect of Abstract -A study was made of the functional role of silencers in the long-distance interaction between insulators. Transgenic lines containing the Su(Hw) and Zw5 insulators were analyzed. The silencers failed to efficiently suppress transcription of the yellow gene, nor did they neutralize the effect of the Su(Hw) insulator. The long-distance in...

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Untitled

Cited by 4 publications

References 36 publications

Mapping the D.melanogaster En1A Enhancer Modules Responsible for Transcription Activation and LongDistance Enhancer-Promoter Interactions

Mapping the D.melanogaster En1A Enhancer Modules Responsible for Transcription Activation and LongDistance Enhancer-Promoter Interactions

Individual and population variation in cercariae of bird schistosomes of the Trichobilharzia ocellata species group as revealed with the polymerase chain reaction

Roles of silencers from the regulatory region of the Abdominal-B gene and the Zw5 and Su(Hw) insulators in regulating gene expression in Drosophila melanogaster

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